Molecular and physiological studies in walnut ( Juglans regia ) are combined to establish the putative role of leaf plasma membrane aquaporins in the response of leaf hydraulic conductance ( K leaf ) to irradiance. The effects of light and temperature on K leaf are described. Under dark conditions, K leaf was low, but increased by 400% upon exposure to light. In contrast to dark conditions, K leaf values of light-exposed leaves responded to temperature and 0.1 m m cycloheximide treatments. Furthermore, K leaf was not related to stomatal aperture. Data of real-time reverse transcription-polymerase chain reaction showed that K leaf dynamics were tightly correlated with the transcript abundance of two walnut aquaporins ( JrPIP2 , 1 and JrPIP2 , 2 ). Low K leaf in the dark was associated with down-regulation, whereas high K leaf in the light was associated with up-regulation of JrPIP2 . Light responses of K leaf and aquaporin transcripts were reversible and inhibited by cycloheximide, indicating the importance of de novo protein biosynthesis in this process. Our results indicate that walnut leaves can rapidly change their hydraulic conductance and suggest that these changes can be explained by regulation of plasma membrane aquaporins. Model simulation suggests that variable leaf hydraulic conductance in walnut might enhance leaf gas exchanges while buffering leaf water status in response to ambient light fluctuations.
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera