Putative Role of Aquaporins in Variable Hydraulic Conductance of Leaves in Response to Light

Putative Role of Aquaporins in Variable Hydraulic Conductance of Leaves in Response to Light Molecular and physiological studies in walnut ( Juglans regia ) are combined to establish the putative role of leaf plasma membrane aquaporins in the response of leaf hydraulic conductance ( K leaf ) to irradiance. The effects of light and temperature on K leaf are described. Under dark conditions, K leaf was low, but increased by 400% upon exposure to light. In contrast to dark conditions, K leaf values of light-exposed leaves responded to temperature and 0.1 m m cycloheximide treatments. Furthermore, K leaf was not related to stomatal aperture. Data of real-time reverse transcription-polymerase chain reaction showed that K leaf dynamics were tightly correlated with the transcript abundance of two walnut aquaporins ( JrPIP2 , 1 and JrPIP2 , 2 ). Low K leaf in the dark was associated with down-regulation, whereas high K leaf in the light was associated with up-regulation of JrPIP2 . Light responses of K leaf and aquaporin transcripts were reversible and inhibited by cycloheximide, indicating the importance of de novo protein biosynthesis in this process. Our results indicate that walnut leaves can rapidly change their hydraulic conductance and suggest that these changes can be explained by regulation of plasma membrane aquaporins. Model simulation suggests that variable leaf hydraulic conductance in walnut might enhance leaf gas exchanges while buffering leaf water status in response to ambient light fluctuations. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

Putative Role of Aquaporins in Variable Hydraulic Conductance of Leaves in Response to Light

Loading next page...
 
/lp/american-society-of-plant-biologist/putative-role-of-aquaporins-in-variable-hydraulic-conductance-of-foEBxpbvn0
Publisher
American Society of Plant Biologist
Copyright
Copyright © 2015 by the American Society of Plant Biologists
ISSN
1532-2548
eISSN
0032-0889
D.O.I.
10.1104/pp.106.090092
Publisher site
See Article on Publisher Site

Abstract

Molecular and physiological studies in walnut ( Juglans regia ) are combined to establish the putative role of leaf plasma membrane aquaporins in the response of leaf hydraulic conductance ( K leaf ) to irradiance. The effects of light and temperature on K leaf are described. Under dark conditions, K leaf was low, but increased by 400% upon exposure to light. In contrast to dark conditions, K leaf values of light-exposed leaves responded to temperature and 0.1 m m cycloheximide treatments. Furthermore, K leaf was not related to stomatal aperture. Data of real-time reverse transcription-polymerase chain reaction showed that K leaf dynamics were tightly correlated with the transcript abundance of two walnut aquaporins ( JrPIP2 , 1 and JrPIP2 , 2 ). Low K leaf in the dark was associated with down-regulation, whereas high K leaf in the light was associated with up-regulation of JrPIP2 . Light responses of K leaf and aquaporin transcripts were reversible and inhibited by cycloheximide, indicating the importance of de novo protein biosynthesis in this process. Our results indicate that walnut leaves can rapidly change their hydraulic conductance and suggest that these changes can be explained by regulation of plasma membrane aquaporins. Model simulation suggests that variable leaf hydraulic conductance in walnut might enhance leaf gas exchanges while buffering leaf water status in response to ambient light fluctuations.

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off