Polyamine Accumulation and Near Loss of Morphogenesis in Long-Term Callus Cultures of Rice (Restoration of Plant Regeneration by Manipulation of Cellular Polyamine Levels)

Polyamine Accumulation and Near Loss of Morphogenesis in Long-Term Callus Cultures of Rice... We have shown (S. Bajaj and M.V. Rajam (1995) Plant Cell Rep 14: 717–720) that a significant reduction in morphogenetic potential occurs in callus cultures of rice (Oryza sativa L. cv TN-1) (up to 1 year old), and that plant regeneration could be improved in such cultures with spermidine treatment. We now show a near loss in plant regeneration capacity, concomitant with massive polyamine accumulation (primarily the diamine putrescine), due to the increase in arginine decarboxylase activity and an altered putrescine-to-spermidine ratio in 20- and 36-month-old rice callus cultures. The blockage of polyamine accumulation due to the reduction in arginine decarboxylase activity by a putrescine synthesis inhibitor, (alpha)-difluoromethylarginine, completely restored plant regeneration capacity in these long-term cultures. Additionally, spermidine treatment of long-term cultures caused an increase in cellular spermidine content and a reduction in putrescine content and arginine decarboxylase activity, leading to an adjustment in putrescine-to-spermidine ratio and the restoration of plant regeneration ability. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

Polyamine Accumulation and Near Loss of Morphogenesis in Long-Term Callus Cultures of Rice (Restoration of Plant Regeneration by Manipulation of Cellular Polyamine Levels)

Nov 1, 1996

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Publisher
American Society of Plant Biologist
Copyright
Copyright © 1996 by the American Society of Plant Biologists
ISSN
1532-2548
eISSN
0032-0889
D.O.I.
10.1104/pp.112.3.1343
Publisher site
See Article on Publisher Site

Abstract

We have shown (S. Bajaj and M.V. Rajam (1995) Plant Cell Rep 14: 717–720) that a significant reduction in morphogenetic potential occurs in callus cultures of rice (Oryza sativa L. cv TN-1) (up to 1 year old), and that plant regeneration could be improved in such cultures with spermidine treatment. We now show a near loss in plant regeneration capacity, concomitant with massive polyamine accumulation (primarily the diamine putrescine), due to the increase in arginine decarboxylase activity and an altered putrescine-to-spermidine ratio in 20- and 36-month-old rice callus cultures. The blockage of polyamine accumulation due to the reduction in arginine decarboxylase activity by a putrescine synthesis inhibitor, (alpha)-difluoromethylarginine, completely restored plant regeneration capacity in these long-term cultures. Additionally, spermidine treatment of long-term cultures caused an increase in cellular spermidine content and a reduction in putrescine content and arginine decarboxylase activity, leading to an adjustment in putrescine-to-spermidine ratio and the restoration of plant regeneration ability.

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