Menachem Moshelion a , Dirk Becker b , Alexander Biela b , Norbert Uehlein b , Rainer Hedrich b , Beate Otto b , Hadas Levi a , Nava Moran 1 , a and Ralf Kaldenhoff b a Department of Agricultural Botany, Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food, and Environmental Quality Sciences, the Hebrew University of Jerusalem, Rehovot 76100, Israel b Julius-von-Sachs-Insitute, Department of Molecular Plant Physiology and Biophysics, Julius-von-Sachs-Platz 2, D97082 Wuerzburg, Germany ↵ 1 To whom correspondence should be addressed. E-mail email@example.com ; fax 972-8-946-7763 Abstract Leaf-moving organs, remarkable for the rhythmic volume changes of their motor cells, served as a model system in which to study the regulation of membrane water fluxes. Two plasma membrane intrinsic protein homolog genes, SsAQP1 and SsAQP2, were cloned from these organs and characterized as aquaporins in Xenopu s laevis oocytes. Osmotic water permeability (P f ) was 10 times higher in SsAQP2-expressing oocytes than in SsAQP1-expressing oocytes. SsAQP1 was found to be glycerol permeable, and SsAQP2 was inhibited by 0.5 mM HgCl 2 and by 1 mM phloretin. The aquaporin mRNA levels differed in their spatial distribution in the leaf and were regulated diurnally in phase with leaflet movements. Additionally, SsAQP2 transcription was under circadian control. The P f of motor cell protoplasts was regulated diurnally as well: the morning and/or evening P f increases were inhibited by 50 μM HgCl 2 , by 2 mM cycloheximide, and by 250 μM phloretin to the noon P f level. Our results link SsAQP2 to the physiological function of rhythmic cell volume changes.
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