Plasma Membrane Aquaporins in the Motor Cells of Samanea saman Diurnal and Circadian Regulation

Plasma Membrane Aquaporins in the Motor Cells of Samanea saman Diurnal and Circadian Regulation Menachem Moshelion a , Dirk Becker b , Alexander Biela b , Norbert Uehlein b , Rainer Hedrich b , Beate Otto b , Hadas Levi a , Nava Moran 1 , a and Ralf Kaldenhoff b a Department of Agricultural Botany, Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food, and Environmental Quality Sciences, the Hebrew University of Jerusalem, Rehovot 76100, Israel b Julius-von-Sachs-Insitute, Department of Molecular Plant Physiology and Biophysics, Julius-von-Sachs-Platz 2, D97082 Wuerzburg, Germany ↵ 1 To whom correspondence should be addressed. E-mail nava.moran@huji.ac.il ; fax 972-8-946-7763 Abstract Leaf-moving organs, remarkable for the rhythmic volume changes of their motor cells, served as a model system in which to study the regulation of membrane water fluxes. Two plasma membrane intrinsic protein homolog genes, SsAQP1 and SsAQP2, were cloned from these organs and characterized as aquaporins in Xenopu s laevis oocytes. Osmotic water permeability (P f ) was 10 times higher in SsAQP2-expressing oocytes than in SsAQP1-expressing oocytes. SsAQP1 was found to be glycerol permeable, and SsAQP2 was inhibited by 0.5 mM HgCl 2 and by 1 mM phloretin. The aquaporin mRNA levels differed in their spatial distribution in the leaf and were regulated diurnally in phase with leaflet movements. Additionally, SsAQP2 transcription was under circadian control. The P f of motor cell protoplasts was regulated diurnally as well: the morning and/or evening P f increases were inhibited by 50 μM HgCl 2 , by 2 mM cycloheximide, and by 250 μM phloretin to the noon P f level. Our results link SsAQP2 to the physiological function of rhythmic cell volume changes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

Loading next page...
 
/lp/american-society-of-plant-biologist/plasma-membrane-aquaporins-in-the-motor-cells-of-samanea-saman-diurnal-dNukZFrWOF
Publisher
American Society of Plant Biologist
Copyright
Copyright © 2015 by the American Society of Plant Biologists
ISSN
1040-4651
eISSN
1532-298X
D.O.I.
10.1105/tpc.010351
Publisher site
See Article on Publisher Site

Abstract

Menachem Moshelion a , Dirk Becker b , Alexander Biela b , Norbert Uehlein b , Rainer Hedrich b , Beate Otto b , Hadas Levi a , Nava Moran 1 , a and Ralf Kaldenhoff b a Department of Agricultural Botany, Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food, and Environmental Quality Sciences, the Hebrew University of Jerusalem, Rehovot 76100, Israel b Julius-von-Sachs-Insitute, Department of Molecular Plant Physiology and Biophysics, Julius-von-Sachs-Platz 2, D97082 Wuerzburg, Germany ↵ 1 To whom correspondence should be addressed. E-mail nava.moran@huji.ac.il ; fax 972-8-946-7763 Abstract Leaf-moving organs, remarkable for the rhythmic volume changes of their motor cells, served as a model system in which to study the regulation of membrane water fluxes. Two plasma membrane intrinsic protein homolog genes, SsAQP1 and SsAQP2, were cloned from these organs and characterized as aquaporins in Xenopu s laevis oocytes. Osmotic water permeability (P f ) was 10 times higher in SsAQP2-expressing oocytes than in SsAQP1-expressing oocytes. SsAQP1 was found to be glycerol permeable, and SsAQP2 was inhibited by 0.5 mM HgCl 2 and by 1 mM phloretin. The aquaporin mRNA levels differed in their spatial distribution in the leaf and were regulated diurnally in phase with leaflet movements. Additionally, SsAQP2 transcription was under circadian control. The P f of motor cell protoplasts was regulated diurnally as well: the morning and/or evening P f increases were inhibited by 50 μM HgCl 2 , by 2 mM cycloheximide, and by 250 μM phloretin to the noon P f level. Our results link SsAQP2 to the physiological function of rhythmic cell volume changes.

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off