Oxidative Remodeling of Chromoplast Carotenoids Identification of the Carotenoid Dioxygenase CsCCD and CsZCD Genes Involved in Crocus Secondary Metabolite Biogenesis

Oxidative Remodeling of Chromoplast Carotenoids Identification of the Carotenoid Dioxygenase... Florence Bouvier a , Claude Suire b , Jérôme Mutterer a and Bilal Camara 1 , a a Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique and Université Louis Pasteur, 67084 Strasbourg Cedex, France b Institut de Biochimie et Génétique Cellulaires, Centre National de la Recherche Scientifique, 33077 Bordeaux Cedex, France ↵ 1 To whom correspondence should be addressed. E-mail bilal.camara@ibmp-ulp.u-strasbg.fr ; fax 33-03-8861-4442 Abstract The accumulation of three major carotenoid derivatives—crocetin glycosides, picrocrocin, and safranal—is in large part responsible for the color, bitter taste, and aroma of saffron, which is obtained from the dried styles of Crocus. We have identified and functionally characterized the Crocus zeaxanthin 7,8(7′,8′)-cleavage dioxygenase gene ( CsZCD ), which codes for a chromoplast enzyme that initiates the biogenesis of these derivatives. The Crocus carotenoid 9,10(9′,10′)-cleavage dioxygenase gene ( CsCCD ) also has been cloned, and the comparison of substrate specificities between these two enzymes has shown that the CsCCD enzyme acts on a broader range of precursors. CsZCD expression is restricted to the style branch tissues and is enhanced under dehydration stress, whereas CsCCD is expressed constitutively in flower and leaf tissues irrespective of dehydration stress. Electron microscopy revealed that the accumulation of saffron metabolites is accompanied by the differentiation of amyloplasts and chromoplasts and by interactions between chromoplasts and the vacuole. Our data suggest that a stepwise sequence exists that involves the oxidative cleavage of zeaxanthin in chromoplasts followed by the sequestration of modified water-soluble derivatives into the central vacuole. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

Oxidative Remodeling of Chromoplast Carotenoids Identification of the Carotenoid Dioxygenase CsCCD and CsZCD Genes Involved in Crocus Secondary Metabolite Biogenesis

Loading next page...
 
/lp/american-society-of-plant-biologist/oxidative-remodeling-of-chromoplast-carotenoids-identification-of-the-Y72JcrvU0K
Publisher
American Society of Plant Biologist
Copyright
Copyright © 2015 by the American Society of Plant Biologists
ISSN
1040-4651
eISSN
1532-298X
D.O.I.
10.1105/tpc.006536
Publisher site
See Article on Publisher Site

Abstract

Florence Bouvier a , Claude Suire b , Jérôme Mutterer a and Bilal Camara 1 , a a Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique and Université Louis Pasteur, 67084 Strasbourg Cedex, France b Institut de Biochimie et Génétique Cellulaires, Centre National de la Recherche Scientifique, 33077 Bordeaux Cedex, France ↵ 1 To whom correspondence should be addressed. E-mail bilal.camara@ibmp-ulp.u-strasbg.fr ; fax 33-03-8861-4442 Abstract The accumulation of three major carotenoid derivatives—crocetin glycosides, picrocrocin, and safranal—is in large part responsible for the color, bitter taste, and aroma of saffron, which is obtained from the dried styles of Crocus. We have identified and functionally characterized the Crocus zeaxanthin 7,8(7′,8′)-cleavage dioxygenase gene ( CsZCD ), which codes for a chromoplast enzyme that initiates the biogenesis of these derivatives. The Crocus carotenoid 9,10(9′,10′)-cleavage dioxygenase gene ( CsCCD ) also has been cloned, and the comparison of substrate specificities between these two enzymes has shown that the CsCCD enzyme acts on a broader range of precursors. CsZCD expression is restricted to the style branch tissues and is enhanced under dehydration stress, whereas CsCCD is expressed constitutively in flower and leaf tissues irrespective of dehydration stress. Electron microscopy revealed that the accumulation of saffron metabolites is accompanied by the differentiation of amyloplasts and chromoplasts and by interactions between chromoplasts and the vacuole. Our data suggest that a stepwise sequence exists that involves the oxidative cleavage of zeaxanthin in chromoplasts followed by the sequestration of modified water-soluble derivatives into the central vacuole.

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off