OsTZF1 is a member of the CCCH-type zinc finger gene family in rice ( Oryza sativa ). Expression of OsTZF1 was induced by drought, high-salt stress, and hydrogen peroxide. OsTZF1 gene expression was also induced by abscisic acid, methyl jasmonate, and salicylic acid. Histochemical activity of β-glucuronidase in transgenic rice plants containing the promoter of OsTZF1 fused with β-glucuronidase was observed in callus, coleoptile, young leaf, and panicle tissues. Upon stress, OsTZF1-green fluorescent protein localization was observed in the cytoplasm and cytoplasmic foci. Transgenic rice plants overexpressing OsTZF1 driven by a maize ( Zea mays ) ubiquitin promoter ( Ubi : OsTZF1- OX (for overexpression)) exhibited delayed seed germination, growth retardation at the seedling stage, and delayed leaf senescence. RNA interference ( RNAi ) knocked-down plants ( OsTZF1 - RNAi ) showed early seed germination, enhanced seedling growth, and early leaf senescence compared with controls. Ubi : OsTZF1- OX plants showed improved tolerance to high-salt and drought stresses and vice versa for OsTZF1- RNAi plants. Microarray analysis revealed that genes related to stress, reactive oxygen species homeostasis, and metal homeostasis were regulated in the Ubi : OsTZF1- OX plants. RNA-binding assays indicated that OsTZF1 binds to U-rich regions in the 3′ untranslated region of messenger RNAs, suggesting that OsTZF1 might be associated with RNA metabolism of stress-responsive genes. OsTZF1 may serve as a useful biotechnological tool for the improvement of stress tolerance in various plants through the control of RNA metabolism of stress-responsive genes. Glossary ABA abscisic acid H 2 O 2 hydrogen peroxide SA salicylic acid JA jasmonic acid PB P body SG stress granule OX overexpression RNAi RNA interference DAI days after imbibition F v / F m maximum photochemical efficiency of PSII in the dark-adapted state ROS reactive oxygen species DAB diaminobenzidine GST glutathione S -transferase qRT quantitative reverse transcription REMSA RNA gel electrophoresis mobility shift assay UTR untranslated region ARE AU-rich element
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera