OsTZF1, a CCCH-Tandem Zinc Finger Protein, Confers Delayed Senescence and Stress Tolerance in Rice by Regulating Stress-Related Genes

OsTZF1, a CCCH-Tandem Zinc Finger Protein, Confers Delayed Senescence and Stress Tolerance in... OsTZF1 is a member of the CCCH-type zinc finger gene family in rice ( Oryza sativa ). Expression of OsTZF1 was induced by drought, high-salt stress, and hydrogen peroxide. OsTZF1 gene expression was also induced by abscisic acid, methyl jasmonate, and salicylic acid. Histochemical activity of β-glucuronidase in transgenic rice plants containing the promoter of OsTZF1 fused with β-glucuronidase was observed in callus, coleoptile, young leaf, and panicle tissues. Upon stress, OsTZF1-green fluorescent protein localization was observed in the cytoplasm and cytoplasmic foci. Transgenic rice plants overexpressing OsTZF1 driven by a maize ( Zea mays ) ubiquitin promoter ( Ubi : OsTZF1- OX (for overexpression)) exhibited delayed seed germination, growth retardation at the seedling stage, and delayed leaf senescence. RNA interference ( RNAi ) knocked-down plants ( OsTZF1 - RNAi ) showed early seed germination, enhanced seedling growth, and early leaf senescence compared with controls. Ubi : OsTZF1- OX plants showed improved tolerance to high-salt and drought stresses and vice versa for OsTZF1- RNAi plants. Microarray analysis revealed that genes related to stress, reactive oxygen species homeostasis, and metal homeostasis were regulated in the Ubi : OsTZF1- OX plants. RNA-binding assays indicated that OsTZF1 binds to U-rich regions in the 3′ untranslated region of messenger RNAs, suggesting that OsTZF1 might be associated with RNA metabolism of stress-responsive genes. OsTZF1 may serve as a useful biotechnological tool for the improvement of stress tolerance in various plants through the control of RNA metabolism of stress-responsive genes. Glossary ABA abscisic acid H 2 O 2 hydrogen peroxide SA salicylic acid JA jasmonic acid PB P body SG stress granule OX overexpression RNAi RNA interference DAI days after imbibition F v / F m maximum photochemical efficiency of PSII in the dark-adapted state ROS reactive oxygen species DAB diaminobenzidine GST glutathione S -transferase qRT quantitative reverse transcription REMSA RNA gel electrophoresis mobility shift assay UTR untranslated region ARE AU-rich element http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

OsTZF1, a CCCH-Tandem Zinc Finger Protein, Confers Delayed Senescence and Stress Tolerance in Rice by Regulating Stress-Related Genes

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Publisher
American Society of Plant Biologists
Copyright
Copyright © 2015 by the American Society of Plant Biologists
ISSN
1532-2548
eISSN
0032-0889
D.O.I.
10.1104/pp.112.205385
Publisher site
See Article on Publisher Site

Abstract

OsTZF1 is a member of the CCCH-type zinc finger gene family in rice ( Oryza sativa ). Expression of OsTZF1 was induced by drought, high-salt stress, and hydrogen peroxide. OsTZF1 gene expression was also induced by abscisic acid, methyl jasmonate, and salicylic acid. Histochemical activity of β-glucuronidase in transgenic rice plants containing the promoter of OsTZF1 fused with β-glucuronidase was observed in callus, coleoptile, young leaf, and panicle tissues. Upon stress, OsTZF1-green fluorescent protein localization was observed in the cytoplasm and cytoplasmic foci. Transgenic rice plants overexpressing OsTZF1 driven by a maize ( Zea mays ) ubiquitin promoter ( Ubi : OsTZF1- OX (for overexpression)) exhibited delayed seed germination, growth retardation at the seedling stage, and delayed leaf senescence. RNA interference ( RNAi ) knocked-down plants ( OsTZF1 - RNAi ) showed early seed germination, enhanced seedling growth, and early leaf senescence compared with controls. Ubi : OsTZF1- OX plants showed improved tolerance to high-salt and drought stresses and vice versa for OsTZF1- RNAi plants. Microarray analysis revealed that genes related to stress, reactive oxygen species homeostasis, and metal homeostasis were regulated in the Ubi : OsTZF1- OX plants. RNA-binding assays indicated that OsTZF1 binds to U-rich regions in the 3′ untranslated region of messenger RNAs, suggesting that OsTZF1 might be associated with RNA metabolism of stress-responsive genes. OsTZF1 may serve as a useful biotechnological tool for the improvement of stress tolerance in various plants through the control of RNA metabolism of stress-responsive genes. Glossary ABA abscisic acid H 2 O 2 hydrogen peroxide SA salicylic acid JA jasmonic acid PB P body SG stress granule OX overexpression RNAi RNA interference DAI days after imbibition F v / F m maximum photochemical efficiency of PSII in the dark-adapted state ROS reactive oxygen species DAB diaminobenzidine GST glutathione S -transferase qRT quantitative reverse transcription REMSA RNA gel electrophoresis mobility shift assay UTR untranslated region ARE AU-rich element

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