Nucleotide and Deduced Acid Amino Acid Sequence of the 22-Kilodalton Cathepsin D Inhibitor Protein of Potato (Solanum tuberosum L.)

Nucleotide and Deduced Acid Amino Acid Sequence of the 22-Kilodalton Cathepsin D Inhibitor... David J.Hannapel* Department of Horticulture, lowa State University, Ames, lowa 5001 1 Severa1 storage proteins of potato (Solanum tuberosum L.) tubers have been identified, including severa1 proteinase inhibitors such as proteinase inhibitor I and I1 and the 22-kD protein group (Melville and Ryan, 1972; Bryant et al., 1976; Suh et al., 1990). The 22-kD cathepsin D inhibitor protein of potatoes (PDI) has been purified and characterized (Mares et al., 1989). This glycosylated protein inhibits cathepsin D and trypsin activity and is homologous with the soybean trypsin inhibitor family (Mares et al., 1989). Recent studies showed that PDI transcripts accumulate in potato tubers, stems, roots, This work was supported by the Iowa State University Biotechnology Council. Journal Paper No. J-14950 of the Iowa Agriculture and Home Economics Experiment Station, Ames, IA. Project No. 3056. * Fax 1-515-294-0730. and leaves depending on genotype and stage of development (Hannapel, 1991). Accumulation of potato cathepsin D inhibitor mRNA is wound inducible in leaves and occurs both locally and systemically (Suh et al., 1991). The PDI cDNA clone p749 was isolated from a tuber cDNA library using differential screening (Table I). The DNA sequence data and the deduced amino acid sequence are shown http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

Nucleotide and Deduced Acid Amino Acid Sequence of the 22-Kilodalton Cathepsin D Inhibitor Protein of Potato (Solanum tuberosum L.)

Feb 19, 1993

Loading next page...
 
/lp/american-society-of-plant-biologist/nucleotide-and-deduced-acid-amino-acid-sequence-of-the-22-kilodalton-JbzCpQ007F
Publisher
American Society of Plant Biologist
Copyright
Copyright © 1993 by the American Society of Plant Biologists
ISSN
1532-2548
eISSN
0032-0889
Publisher site
See Article on Publisher Site

Abstract

David J.Hannapel* Department of Horticulture, lowa State University, Ames, lowa 5001 1 Severa1 storage proteins of potato (Solanum tuberosum L.) tubers have been identified, including severa1 proteinase inhibitors such as proteinase inhibitor I and I1 and the 22-kD protein group (Melville and Ryan, 1972; Bryant et al., 1976; Suh et al., 1990). The 22-kD cathepsin D inhibitor protein of potatoes (PDI) has been purified and characterized (Mares et al., 1989). This glycosylated protein inhibits cathepsin D and trypsin activity and is homologous with the soybean trypsin inhibitor family (Mares et al., 1989). Recent studies showed that PDI transcripts accumulate in potato tubers, stems, roots, This work was supported by the Iowa State University Biotechnology Council. Journal Paper No. J-14950 of the Iowa Agriculture and Home Economics Experiment Station, Ames, IA. Project No. 3056. * Fax 1-515-294-0730. and leaves depending on genotype and stage of development (Hannapel, 1991). Accumulation of potato cathepsin D inhibitor mRNA is wound inducible in leaves and occurs both locally and systemically (Suh et al., 1991). The PDI cDNA clone p749 was isolated from a tuber cDNA library using differential screening (Table I). The DNA sequence data and the deduced amino acid sequence are shown

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off