Mutant Resources in Rice for Functional Genomics of the Grasses

Mutant Resources in Rice for Functional Genomics of the Grasses Insertion Mutants With the sequencing of plant genomes, it was recognized that insertion mutants indexed by their position in the genome would be very suitable for systematic analysis of annotated genes by reverse genetics (Parinov and Sundaresan, 2000 ). Arabidopsis ( Arabidopsis thaliana ) genome sequence-indexed mutants can now be found in databases ( http://signal.salk.edu/Source/AtTOME_Data_Source.html ), which comprise a total of 379,674 inserts tagging 30,280 of the predicted 33,003 genes. In rice, the two-component maize transposon Ac - Ds (Chin et al., 1999 ; Upadhyaya et al., 2002 ; Greco et al., 2003 ; Kolesnik et al., 2004 ) and En / Spm-dSpm (Greco et al., 2004 ; Kumar et al., 2008 ) systems have been well characterized, and good genetic selection schemes have been developed to select for transpositional activity useful for large-scale mutagenesis (Hirochika et al., 2004 ; Zhu et al., 2007 ). In addition, the endogenous rice Tos17 retrotransposon is active in specific genotypes and conditions and is an effective insertion mutagen in the rice genome (Miyao et al., 2003 ). The development of efficient protocols for rice transformation has helped in the generation of a large number of transgenic rice plants bearing low-copy T-DNA http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

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Publisher
American Society of Plant Biologist
Copyright
Copyright © 2015 by the American Society of Plant Biologists
ISSN
1532-2548
eISSN
0032-0889
D.O.I.
10.1104/pp.108.128918
Publisher site
See Article on Publisher Site

Abstract

Insertion Mutants With the sequencing of plant genomes, it was recognized that insertion mutants indexed by their position in the genome would be very suitable for systematic analysis of annotated genes by reverse genetics (Parinov and Sundaresan, 2000 ). Arabidopsis ( Arabidopsis thaliana ) genome sequence-indexed mutants can now be found in databases ( http://signal.salk.edu/Source/AtTOME_Data_Source.html ), which comprise a total of 379,674 inserts tagging 30,280 of the predicted 33,003 genes. In rice, the two-component maize transposon Ac - Ds (Chin et al., 1999 ; Upadhyaya et al., 2002 ; Greco et al., 2003 ; Kolesnik et al., 2004 ) and En / Spm-dSpm (Greco et al., 2004 ; Kumar et al., 2008 ) systems have been well characterized, and good genetic selection schemes have been developed to select for transpositional activity useful for large-scale mutagenesis (Hirochika et al., 2004 ; Zhu et al., 2007 ). In addition, the endogenous rice Tos17 retrotransposon is active in specific genotypes and conditions and is an effective insertion mutagen in the rice genome (Miyao et al., 2003 ). The development of efficient protocols for rice transformation has helped in the generation of a large number of transgenic rice plants bearing low-copy T-DNA

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