Insertion Mutants With the sequencing of plant genomes, it was recognized that insertion mutants indexed by their position in the genome would be very suitable for systematic analysis of annotated genes by reverse genetics (Parinov and Sundaresan, 2000 ). Arabidopsis ( Arabidopsis thaliana ) genome sequence-indexed mutants can now be found in databases ( http://signal.salk.edu/Source/AtTOME_Data_Source.html ), which comprise a total of 379,674 inserts tagging 30,280 of the predicted 33,003 genes. In rice, the two-component maize transposon Ac - Ds (Chin et al., 1999 ; Upadhyaya et al., 2002 ; Greco et al., 2003 ; Kolesnik et al., 2004 ) and En / Spm-dSpm (Greco et al., 2004 ; Kumar et al., 2008 ) systems have been well characterized, and good genetic selection schemes have been developed to select for transpositional activity useful for large-scale mutagenesis (Hirochika et al., 2004 ; Zhu et al., 2007 ). In addition, the endogenous rice Tos17 retrotransposon is active in specific genotypes and conditions and is an effective insertion mutagen in the rice genome (Miyao et al., 2003 ). The development of efficient protocols for rice transformation has helped in the generation of a large number of transgenic rice plants bearing low-copy T-DNA
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera