Malate Oxidation, Rotenone-Resistance, and Alternative Path Activity in Plant Mitochondria

Malate Oxidation, Rotenone-Resistance, and Alternative Path Activity in Plant Mitochondria The effect of cyanide and rotenone on malate (pH 6.8), malate plus glutamate (pH 7.8), citrate, α-ketoglutarate, and succinate oxidation by cauliflower ( Brassica oleracea L.) bud, sweet potato ( Ipomoea batatis L.) tuber, and spinach ( Spinacia oleracea and Kalanchoë daigremontiana leaf mitochondria was investigated. Cyanide inhibited all substrates equally with the exception of malate plus glutamate; in this case, inhibition of O 2 uptake was more severe due to an effect of cyanide on aspartate aminotransferase. Azide and antimycin A gave similar inhibitions with all substrates. Subsequent addition of NAD had no effect with any substrate. Providing that oxalacetate accumulation was prevented, rotenone inhibited all NAD-linked substrates equally and caused ADP:O ratios to decrease by one-third. Addition of succinate to mitochondria oxidizing malate stimulated oxygen uptake, but adding citrate and α-ketoglutarate did not. These results indicate that there is no direct link between malic enzyme and the rotenone- and cyanide-resistant respiratory pathways, and that there is no need to postulate separate compartmentation of malic enzyme and the other NAD-linked enzymes in the matrix. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

Malate Oxidation, Rotenone-Resistance, and Alternative Path Activity in Plant Mitochondria

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Publisher
American Society of Plant Biologist
Copyright
Copyright © 1982 by the American Society of Plant Biologists
ISSN
1532-2548
eISSN
0032-0889
DOI
10.1104/pp.70.4.959
Publisher site
See Article on Publisher Site

Abstract

The effect of cyanide and rotenone on malate (pH 6.8), malate plus glutamate (pH 7.8), citrate, α-ketoglutarate, and succinate oxidation by cauliflower ( Brassica oleracea L.) bud, sweet potato ( Ipomoea batatis L.) tuber, and spinach ( Spinacia oleracea and Kalanchoë daigremontiana leaf mitochondria was investigated. Cyanide inhibited all substrates equally with the exception of malate plus glutamate; in this case, inhibition of O 2 uptake was more severe due to an effect of cyanide on aspartate aminotransferase. Azide and antimycin A gave similar inhibitions with all substrates. Subsequent addition of NAD had no effect with any substrate. Providing that oxalacetate accumulation was prevented, rotenone inhibited all NAD-linked substrates equally and caused ADP:O ratios to decrease by one-third. Addition of succinate to mitochondria oxidizing malate stimulated oxygen uptake, but adding citrate and α-ketoglutarate did not. These results indicate that there is no direct link between malic enzyme and the rotenone- and cyanide-resistant respiratory pathways, and that there is no need to postulate separate compartmentation of malic enzyme and the other NAD-linked enzymes in the matrix.

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