Maize Mutants for the 2Ist Century Mutants provide the primary means of Knl defines the first homeobox-conunderstanding gene function in higher taining plant gene (Vollbrecht et al., plants. At the 1991 Maize Genetics 1991). Using the Knl homeobox as a Meeting, which was held in Delavan, hybridization probe, Brenda Lowe and Wisconsin, from March 21 to March coworkers in Sarah Hakeâs laboratory 24, a practical new strategy for stream- (Plant Gene Expression Center, Allining the identification and mapping of bany, CA) and Michael Freelingâs labomutants was presented. The âinte- ratory (University of California, Berkegrated mapping projectâ will produce a ley) have cloned additional homeoboxsingle high-resolution map of the maize containing genes. Phil Becraft and cogenome that combines physical, restric- workers in the Freeling laboratory tion fragment length polymorphism showed that one of these homeobox (RFLP), and gene markers. This project sequences maps to the same position a is a major undertaking for the maize as Rough sheafh-1 (Rsl), locus community, with the rallying cry â20,000 defined by an EMS-induced dominant loci by the year 2000!â In this report, I leaf mutation. They have cloned and will explain the integrated mapping partially sequenced the gene that project and highlight
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