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Methods Tomiiato (Lycopersicutnt escldentutn variety, Best of All), lettuce (Lactu sativa variety, Imperial 847), and Bermuda grass (Cynodon dactylon) were germinated in salnd in the greenhouse. Upon reaching a size convenient for transplanting thev were transferred to corks in iron tanks (2), filled with a complete nlutrient solution (table I, no. 1). The plants were kept in the complete n-utrient solution for 5 weeks. By allowing the nutrient solution to approach depletion at the end of this period the plants were put in a "low-salt" condition conducive to active absorption (5). At the end of this period they were selected for uniformity and placed for 3 days in a "'minus-ealcium"' solution (table I, no. 2). Since no attempt was made to 1 Acknowledgment is made of cleril assistance given in the preparation of this manuscript by the personnel of the Work Projects Administration, Project #65-1-0891-B-10. 515 remove lcium contaminants, however, the ''miius-lciumii" solutioni eould not be regarded as entirely free from lcium. The healthy appearaniee of the roots at the time of transfer anid their subsequenit pacity for absorption indited that their immersioni for 3 davs in the ''minus-lcium" solution did not result in injury. The purpose of http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Physiology American Society of Plant Biologist
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