on Spruce Tissue Cultures'2 4, Laurens Anderson 5, & Folke Skoog4 Departments of Botany & Biochemistry, University of Wisconsin, Madison Since the publication of Eastcott's work in 1928 (6). myo-inositol has been recognized as a growth factor for a number of micro-organisms. Altugh prol)ably of universal occurrence in plants and aninmals, its role in higher plants is still only partly understoo(l. Similarly, nmany of the isomers an(l derivatives of invo-inositol are commonly found in nature, vet until recently no function has been ascribed to any of these. The term, inositol, used witut a prefix implies the nv1 o-isomler, also referred to as JCesoinositol in the older literature. The isomeric inositols and their (lerivatives all belong t) the larger group of conlpounds collectively known as cyclitols; this group recently has been reviewed Ibv Ang^,yal and Anderson invo-Inositol frequently is added to culture media for plant tissues: there are only a few reports, wever, of its actually being require(l by the tissues. Cultures derived fromn normal carrot tissue and Scorsonera crown gall (11) did not respond to mv1oinositol when thiamiin. biotin, an(l in(loleacetic acid were omitted from the medium; the inositol, wever, greatly enhance(l the activity of the combined treatment
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