Functional architecture of the light-responsive chalcone synthase promoter from parsley.

Functional architecture of the light-responsive chalcone synthase promoter from parsley. We have combined in vivo genomic footprinting and light-induced transient expression of chalcone synthase promoter derivatives in parsley protoplasts to identify cis sequences regulating light activation. The parsley chalcone synthase promoter contains two cis "units" that are light-responsive. Each unit is composed of short DNA stretches of approximately 50 base pairs, and each contains two in vivo footprints. One of the footprints in each unit covers a sequence that is highly conserved among other light- and stress-regulated plant genes. The other footprinted sequences in each unit are not related to each other. The TATA distal light-responsive unit is inherently weak but can compensate partially for the loss of the stronger TATA proximal unit. Levels of light-induced expression from either can be influenced by the presence of a region of approximately 100 base pairs located upstream of the TATA distal light-responsive unit. Combination of the light-responsive units and upstream region generates a synergistic response to light. We speculate that functional compensation generated by nonidentical, but sequence-related, cis units foreshadows combinatorial diversity of cognate trans factors. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

Functional architecture of the light-responsive chalcone synthase promoter from parsley.

Loading next page...
 
/lp/american-society-of-plant-biologist/functional-architecture-of-the-light-responsive-chalcone-synthase-xOIdREJvhO
Publisher
American Society of Plant Biologist
Copyright
Copyright © 1989 by the American Society of Plant Biologists
ISSN
1040-4651
eISSN
1532-298X
D.O.I.
10.1105/tpc.1.7.707
Publisher site
See Article on Publisher Site

Abstract

We have combined in vivo genomic footprinting and light-induced transient expression of chalcone synthase promoter derivatives in parsley protoplasts to identify cis sequences regulating light activation. The parsley chalcone synthase promoter contains two cis "units" that are light-responsive. Each unit is composed of short DNA stretches of approximately 50 base pairs, and each contains two in vivo footprints. One of the footprints in each unit covers a sequence that is highly conserved among other light- and stress-regulated plant genes. The other footprinted sequences in each unit are not related to each other. The TATA distal light-responsive unit is inherently weak but can compensate partially for the loss of the stronger TATA proximal unit. Levels of light-induced expression from either can be influenced by the presence of a region of approximately 100 base pairs located upstream of the TATA distal light-responsive unit. Combination of the light-responsive units and upstream region generates a synergistic response to light. We speculate that functional compensation generated by nonidentical, but sequence-related, cis units foreshadows combinatorial diversity of cognate trans factors.

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off