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Effects of Indoleacetic Acid and Other Oxidation Regulators on in Vitro Peroxidation and Experimental Conversion of Eugenol to Lignin

MATERIAL AND METHODS BIOASSAY SYSTEM: Vascular bundles of celery (Apiutn graveolens) removed as long strands, virtually free of cortical tissue, are used as a model lignifying system. The composition and properties of the synthetic lignin and its soluble derivative have been reported (18). The product approximates natural lignins (1, 3). The soluble derivative appears to consist of trimeric units containing double linked phenylpropane residues, as in the Freudenberg benzofuran structure. This work is in accord with the proposed conversion of eugenol to lignin via coniferaldehyde (1, 10). Excised tissue was washed in cold M/15 phosphate buffer (pH 6.0) and stored at 50 C on moist filter paper until used. Excised tissues, pooled and randomized in batches of 10 to 25 gm (wet weight), could be stored and used without change in activity over periods of three to five days. Experimental samples of 500 mg (±*1 %) were shaken at 25±0.10 C for 15 hrs in 25 ml 0.2 M KH2PO4(pH 4.5) containing eugenol (0.5 mM) and H209 (1.0 mM). Drifts in pH were negligible compared to the spread of the optimum plateau, pH 4 to 6. Tissues to be analyzed for lignin were first rinsed in running water http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Physiology American Society of Plant Biologist

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