Cyanide-insensitive Respiration in Plant Mitochondria

Cyanide-insensitive Respiration in Plant Mitochondria Pathways of electron transport have been studied in mitochondria isolated from hypocotyls of etiolated mung bean seedlings and skunk cabbage spadices that show cyanide-resistant respiratory activity. The residual flux through cytochrome c oxidase is shown to be small in comparison with the flux through an unidentified alternative oxidase that is known to have a high affinity for oxygen. This alternative oxidase is not a cytochrome. Skunk cabbage and mung bean mitochondria contain cytochromes a and a 3 that have absorption peaks differing slightly from those of animal preparations. A slow oxidation-reduction of cytochrome a 3 -CN has been demonstrated. Cytochromes b undergo oxidation and reduction in the presence of cyanide but play no essential role in the cyanide-resistant pathway. Antimycin inhibits to an extent similar to that of cyanide; the respiratory chain bifurcates on the substrate side of the antimycin-sensitive site. Evidence is presented for the selective inhibition by thiocyanate, α, α′-dipyridyl, and 8-hydroxyquinoline of the alternative oxidase pathway, which may therefore contain a non-heme iron protein. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

Cyanide-insensitive Respiration in Plant Mitochondria

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Publisher
American Society of Plant Biologist
Copyright
Copyright © 1971 by the American Society of Plant Biologists
ISSN
1532-2548
eISSN
0032-0889
DOI
10.1104/pp.47.2.236
Publisher site
See Article on Publisher Site

Abstract

Pathways of electron transport have been studied in mitochondria isolated from hypocotyls of etiolated mung bean seedlings and skunk cabbage spadices that show cyanide-resistant respiratory activity. The residual flux through cytochrome c oxidase is shown to be small in comparison with the flux through an unidentified alternative oxidase that is known to have a high affinity for oxygen. This alternative oxidase is not a cytochrome. Skunk cabbage and mung bean mitochondria contain cytochromes a and a 3 that have absorption peaks differing slightly from those of animal preparations. A slow oxidation-reduction of cytochrome a 3 -CN has been demonstrated. Cytochromes b undergo oxidation and reduction in the presence of cyanide but play no essential role in the cyanide-resistant pathway. Antimycin inhibits to an extent similar to that of cyanide; the respiratory chain bifurcates on the substrate side of the antimycin-sensitive site. Evidence is presented for the selective inhibition by thiocyanate, α, α′-dipyridyl, and 8-hydroxyquinoline of the alternative oxidase pathway, which may therefore contain a non-heme iron protein.

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