Auxin Input Pathway Disruptions Are Mitigated by Changes in Auxin Biosynthetic Gene Expression in Arabidopsis

Auxin Input Pathway Disruptions Are Mitigated by Changes in Auxin Biosynthetic Gene Expression in... Auxin is a phytohormone involved in cell elongation and division. Levels of indole-3-acetic acid ( IAA ), the primary auxin, are tightly regulated through biosynthesis, degradation, sequestration, and transport. IAA is sequestered in reversible processes by adding amino acids, polyol or simple alcohols, or sugars, forming IAA conjugates, or through a two-carbon elongation forming indole-3-butyric acid. These sequestered forms of IAA alter hormone activity. To gain a better understanding of how auxin homeostasis is maintained, we have generated Arabidopsis ( Arabidopsis thaliana ) mutants that combine disruptions in the pathways, converting IAA conjugates and indole-3-butyric acid to free IAA . These mutants show phenotypes indicative of low auxin levels, including delayed germination, abnormal vein patterning, and decreased apical dominance. Root phenotypes include changes in root length, root branching, and root hair growth. IAA levels are reduced in the cotyledon tissue but not meristems or hypocotyls. In the combination mutants, auxin biosynthetic gene expression is increased, particularly in the YUCCA / Tryptophan Aminotransferase of Arabidopsis1 pathway, providing a feedback mechanism that allows the plant to compensate for changes in IAA input pathways and maintain cellular homeostasis. Glossary ACC 1-aminocyclopropane-1-carboxylic acid IAA indole-3-acetic acid IBA indole-3-butyric acid MeIAA methyl indole-3-acetic acid PNS plant nutrient media with 0.5% Suc http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

Auxin Input Pathway Disruptions Are Mitigated by Changes in Auxin Biosynthetic Gene Expression in Arabidopsis

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Publisher
American Society of Plant Biologist
Copyright
Copyright © 2015 by the American Society of Plant Biologists
ISSN
1532-2548
eISSN
0032-0889
DOI
10.1104/pp.114.236026
Publisher site
See Article on Publisher Site

Abstract

Auxin is a phytohormone involved in cell elongation and division. Levels of indole-3-acetic acid ( IAA ), the primary auxin, are tightly regulated through biosynthesis, degradation, sequestration, and transport. IAA is sequestered in reversible processes by adding amino acids, polyol or simple alcohols, or sugars, forming IAA conjugates, or through a two-carbon elongation forming indole-3-butyric acid. These sequestered forms of IAA alter hormone activity. To gain a better understanding of how auxin homeostasis is maintained, we have generated Arabidopsis ( Arabidopsis thaliana ) mutants that combine disruptions in the pathways, converting IAA conjugates and indole-3-butyric acid to free IAA . These mutants show phenotypes indicative of low auxin levels, including delayed germination, abnormal vein patterning, and decreased apical dominance. Root phenotypes include changes in root length, root branching, and root hair growth. IAA levels are reduced in the cotyledon tissue but not meristems or hypocotyls. In the combination mutants, auxin biosynthetic gene expression is increased, particularly in the YUCCA / Tryptophan Aminotransferase of Arabidopsis1 pathway, providing a feedback mechanism that allows the plant to compensate for changes in IAA input pathways and maintain cellular homeostasis. Glossary ACC 1-aminocyclopropane-1-carboxylic acid IAA indole-3-acetic acid IBA indole-3-butyric acid MeIAA methyl indole-3-acetic acid PNS plant nutrient media with 0.5% Suc

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