AtDUR3 Encodes a New Type of High-Affinity Urea/H+ Symporter in Arabidopsis

AtDUR3 Encodes a New Type of High-Affinity Urea/H+ Symporter in Arabidopsis Urea is the major nitrogen form supplied as fertilizer in agricultural plant production but also an important nitrogen metabolite in plants. We report the cloning and functional characterization of AtDUR3, a high-affinity urea transporter in plants. AtDUR3 contains 14 putative transmembrane-spanning domains and represents an individual member in Arabidopsis that belongs to a superfamily of sodium-solute symporters. Heterologous expression in urea uptake–defective yeast as well as two-electrode voltage clamp and uptake studies using 14 C-labeled urea in AtDUR3-expressing oocytes demonstrated that AtDUR3 mediates urea transport. In both heterologous systems, urea transport was stimulated at low pH. In oocytes, inward currents indicated that urea is cotransported with protons. By contrast, a supply of Na + ions could not stimulate urea transport. Transport of 14 C-labeled urea by AtDUR3 in oocytes exhibited saturation kinetics with a K m of ∼3 μM. AtDUR3 was expressed in shoots and roots and upregulated during early germination and under nitrogen deficiency in roots. We propose a role of AtDUR3 in urea uptake by plant cells at low external urea concentrations. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

AtDUR3 Encodes a New Type of High-Affinity Urea/H+ Symporter in Arabidopsis

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Publisher
American Society of Plant Biologist
Copyright
Copyright © 2015 by the American Society of Plant Biologists
ISSN
1040-4651
eISSN
1532-298X
D.O.I.
10.1105/tpc.007120
Publisher site
See Article on Publisher Site

Abstract

Urea is the major nitrogen form supplied as fertilizer in agricultural plant production but also an important nitrogen metabolite in plants. We report the cloning and functional characterization of AtDUR3, a high-affinity urea transporter in plants. AtDUR3 contains 14 putative transmembrane-spanning domains and represents an individual member in Arabidopsis that belongs to a superfamily of sodium-solute symporters. Heterologous expression in urea uptake–defective yeast as well as two-electrode voltage clamp and uptake studies using 14 C-labeled urea in AtDUR3-expressing oocytes demonstrated that AtDUR3 mediates urea transport. In both heterologous systems, urea transport was stimulated at low pH. In oocytes, inward currents indicated that urea is cotransported with protons. By contrast, a supply of Na + ions could not stimulate urea transport. Transport of 14 C-labeled urea by AtDUR3 in oocytes exhibited saturation kinetics with a K m of ∼3 μM. AtDUR3 was expressed in shoots and roots and upregulated during early germination and under nitrogen deficiency in roots. We propose a role of AtDUR3 in urea uptake by plant cells at low external urea concentrations.

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