A Statistical Evaluation of a Growth Substance Bioassay Method Using Extracts of Dormant Peach Buds.

A Statistical Evaluation of a Growth Substance Bioassay Method Using Extracts of Dormant Peach Buds. 1 Received November 12, 1957. locations on the base line of the chromatographic paper. Each location or spot of extract represented 1 g fresh weight of buds. Descending chromatography on Whatman No. 1 paper was used throughout this study. The paper was equilibrated for at least 10 hours before the solvent (butanol : ammonia: water 10: 1: 1) was added. After the solvent had descended about 20 cm the paper was removed from the chamber and dried in the laboratory at room temperature using subdued light. After drying, the paper was cut into 17 equal sections including the original spot. For an untreated control a piece of paper the same size as the other sections was cut, usually above the base line. One exception to this is mentioned in the results section. The pieces of paper were then placed in small vials (12 x 60 mm) with 1 ml of a phosphate-citrate buffer (pH 5.0) and 2 % sucrose. Before the coleoptiles were added the vials were shaken for 30 minutes to elute from the paper any growth substances that may be present. The wheat coleoptile straight growth test was used for the bioassay. The variety used was http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

A Statistical Evaluation of a Growth Substance Bioassay Method Using Extracts of Dormant Peach Buds.

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Publisher
American Society of Plant Biologist
Copyright
Copyright © 1958 by the American Society of Plant Biologists
ISSN
1532-2548
eISSN
0032-0889
Publisher site
See Article on Publisher Site

Abstract

1 Received November 12, 1957. locations on the base line of the chromatographic paper. Each location or spot of extract represented 1 g fresh weight of buds. Descending chromatography on Whatman No. 1 paper was used throughout this study. The paper was equilibrated for at least 10 hours before the solvent (butanol : ammonia: water 10: 1: 1) was added. After the solvent had descended about 20 cm the paper was removed from the chamber and dried in the laboratory at room temperature using subdued light. After drying, the paper was cut into 17 equal sections including the original spot. For an untreated control a piece of paper the same size as the other sections was cut, usually above the base line. One exception to this is mentioned in the results section. The pieces of paper were then placed in small vials (12 x 60 mm) with 1 ml of a phosphate-citrate buffer (pH 5.0) and 2 % sucrose. Before the coleoptiles were added the vials were shaken for 30 minutes to elute from the paper any growth substances that may be present. The wheat coleoptile straight growth test was used for the bioassay. The variety used was

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