Abstract

Receive: RSS Feeds, eTOCs, free email alerts (when new articles cite this article), more» Information about commercial reprint orders: http://aem.asm.org/site/misc/reprints.xhtml To subscribe to to another ASM Journal go to: http://journals.asm.org/site/subscriptions/ The i F. J. SIMPSON2 AND ELIZABETH MCCOY Department of Bacteriology, University of Wisconsin, Madison, Wisconsin Received for publication April 27, 1953 Of the 73 Streptomyces spp. described in the 1948 edition of Bergey's Manual of Determinative Bacteriology, 48 are listed as capable of hydrolyzing starch. Little, however, is known about the properties of the amylases of this group of s. Bois and Savary (1945) claim to have identified gentiobiose as a product of the amylolytic action of Streptomyces microflavus on wheat starch. Waksman (1950) reported that Surovaya obtained a potent amylase active at 70 to 100 C and with an optimum at pH 6.6 to 6.7 from an described as Streptomyces diastaticus. None of the five amylases studied in the present investigation was found to be heat stable. MATERIALS AND METHODS Cultures. Four of the cultures used were 3313, S. diastaticus 3315, Stretomyces griseus 4 and S. mnicroflavus 3332 of the Waksman collection. The fifth culture, a , was isolated from compost by starch enrichment. The grew