THE AMINO ACID NUTRITION OF YEAST IN RELATIONSHIP TO BIOTIN DEFICIENCY
Abstract
CONTENT ALERTS Receive: RSS Feeds, eTOCs, free email alerts (when new articles cite this article), more» Information about commercial reprint orders: http://jb.asm.org/site/misc/reprints.xhtml To subscribe to to another ASM Journal go to: http://journals.asm.org/site/subscriptions/ THE AMINO ACID NUTRITION OF YEAST IN RELATIONSHIP TO BIOTIN DEFICIENCY A. G. MOAT AND E. K. EMMONS Department of Microbiology, Hahnemann Medical College, Philadelphia, Pennsylvania Received for publication June 1, 1954 mpg biotin per ml are reported here, all experiments were conducted as a standard vitamin assay through a range of biotin concentrations from 0.001 mpg to 0.1 Ag to insure a normal response to biotin under each of the conditions employed. It should be noted that ammonium sulfate, hereafter referred to asammonia, was included in the basal medium in all experiments. The concentrations of the amino acids employed in this study are given in table 1. Growth was measured turbidimetrically on an Evelyn colorMATERIALS AND METHODS imeter at 500-540 mju wavelength after 26 hr of Saccharomyces cerevuiae, Fleischmann strain incubation at 30-32 C and is expressed as optical 139, was employed as the test organism. The density. inoculum culture was grown for one transfer (24 RESULTS hr at 30-32 C) in the basal medium