Abstract

Surface Analyses and Immune Reactivities of Major Cell Wall-Associated Proteins of Group A Streptococcus Jason N. Cole 1 , Ruben D. Ramirez 1 , Bart J. Currie 2 , Stuart J. Cordwell 3 , Steven P. Djordjevic 4 and Mark J. Walker 1 , * 1 School of Biological Sciences, University of Wollongong, Wollongong, NSW 2 Menzies School of Health Research, Charles Darwin University, Darwin, NT 3 Australian Proteome Analysis Facility, Macquarie University, Sydney, NSW 4 NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Camden, NSW, Australia ABSTRACT A proteomic analysis was undertaken to identify cell wall-associated proteins of Streptococcus pyogenes . Seventy-four distinct cell wall-associated proteins were identified, 66 of which were novel. Thirty-three proteins were immunoreactive with pooled S. pyogenes -reactive human antisera. Biotinylation of the GAS cell surface identified 23 cell wall-associated proteins that are surface exposed.