Abstract

Phylogenetic Characterization of 16S rRNA Gene Clones from Deep-Groundwater Microorganisms That Pass through 0.2-Micrometer-Pore-Size Filters Tatsuo Miyoshi 1 , Teruki Iwatsuki 2 and Takeshi Naganuma 1 , 2 , * 1 Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 2 Tono Geoscience Center, Japan Nuclear Cycle Development Institute, Izumicho, Toki, Japan ABSTRACT A total of 247 clones of 16S rRNA genes from microorganisms captured by 0.2- and 0.1-μm-pore-size filters from sedimentary and granite rock aquifers were amplified and yielded 37 operational taxonomic units (OTUs). Fifteen OTUs captured by 0.1-μm-pore-size filters were affiliated with the candidate divisions OD1 and OP11, representing novel lineages. On the other hand, OTUs captured by 0.2-μm-pore-size filters were largely affiliated with Betaproteobacteria .