Abstract

Participation of subgenomic retroviral mRNAs in recombination. L H Wang and D W Stacey ABSTRACT Envelope glycoprotein (env) mRNA from avian retroviruses was injected into cells transformed by env-deleted Bryan Rous sarcoma virus (RSV(-)). The genetic deficiency of RSV(-) was complemented, and infectious transforming virus was released for many days after these injections. The long-term activity of the injected env mRNA is believed to be due to reverse transcription of the injected RNA after its incorporation into virus particles. The resulting subgenomic provirus, presumed to be integrated into host DNA, is able to direct the continuous synthesis of additional env mRNA. In some of these cultures, replication-competent viruses appeared many days after injection. The analysis by RNase T1 oligonucleotide fingerprinting showed that the RNA of these virus genomes contained oligonucleotides characteristic of both RSV(-) and the env mRNA injected. In all viruses analyzed the 5' two-thirds and the 3' terminus of the genome were derived from RSV(-) and the env gene from the injected mRNA. Our results thus strongly indicate that these viruses were generated via recombination between RSV(-) and env mRNA. The demonstration of involvement of an mRNA sequence in recombination may be of importance in the divergence of retroviruses and in the mechanism of interaction between retroviruses and host nucleotide sequences. CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? « Previous | Next Article » Table of Contents This Article J. Virol. March 1982 vol. 41 no. 3 919-930 » Abstract PDF Classifications Research Article Services Email this article to a colleague Similar articles in ASM journals Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of JVI Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Load citing article information Citing articles via Web of Science Citing articles via Google Scholar Google Scholar Articles by Wang, L. H. Articles by Stacey, D. W. Search for related content PubMed PubMed citation Articles by Wang, L. H. Articles by Stacey, D. W. Related Content Load related web page information Social Bookmarking CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? current issue January 2012, volume 86, issue 1 Spotlights in the Current Issue Two Xenotropic Murine Leukemia Virus Parents Breaking the Entry Targeting Barrier Complex Morphology and Dynamic Development of Poliovirus Membranous Replication Structures Revealed A Staining Artifact Explains Apparent Varicella-Zoster Virus Protein Expression in Neurons Recent Mumps Outbreaks Are Not Caused by Immune Escape Alert me to new issues of JVI About JVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0022-538X Online ISSN: 1098-5514 Copyright © 2011 by the American Society for Microbiology. For an alternate route to JVI .asm.org, visit: http://intl- JVI .asm.org | More Info» var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www."); document.write(unescape("%3Cscript src='" + gaJsHost + "google-analytics.com/ga.js' type='text/javascript'%3E%3C/script%3E")); var pageTracker = _gat._getTracker("UA-5821458-1"); pageTracker._trackPageview();