Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

NS1′ of Flaviviruses in the Japanese Encephalitis Virus Serogroup Is a Product of Ribosomal Frameshifting and Plays a Role in Viral Neuroinvasiveness

NS1′ of Flaviviruses in the Japanese Encephalitis Virus Serogroup Is a Product of Ribosomal... NS1′ of Flaviviruses in the Japanese Encephalitis Virus Serogroup Is a Product of Ribosomal Frameshifting and Plays a Role in Viral Neuroinvasiveness ▿ Ezequiel Balmori Melian 1 , Edward Hinzman 1 , ‡ , Tomoko Nagasaki 1 , Andrew E. Firth 2 , Norma M. Wills 3 , Amanda S. Nouwens 5 , Bradley J. Blitvich 4 , Jason Leung 1 , † , Anneke Funk 1 , John F. Atkins 2 , 3 , Roy Hall 1 and Alexander A. Khromykh 1 , * 1 Centre for Infectious Disease Research, School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, QLD 4072, Australia 2 BioSciences Institute, University College Cork, Cork, Ireland 3 Department of Human Genetics, University of Utah, Salt Lake City, Utah 84112-5330 4 Iowa State University, Ames, Iowa 5 Australian Institute of Bioengineering and Nanotechnology, University of Queensland, Brisbane, QLD 4072, Australia ABSTRACT Flavivirus NS1 is a nonstructural protein involved in virus replication and regulation of the innate immune response. Interestingly, a larger NS1-related protein, NS1′, is often detected during infection with the members of the Japanese encephalitis virus serogroup of flaviviruses. However, how NS1′ is made and what role it performs in the viral life cycle have not been determined. Here we provide experimental evidence that NS1′ is the product of a −1 ribosomal frameshift event that occurs at a conserved slippery heptanucleotide motif located near the beginning of the NS2A gene and is stimulated by a downstream RNA pseudoknot structure. Using site-directed mutagenesis of these sequence elements in an infectious clone of the Kunjin subtype of West Nile virus, we demonstrate that NS1′ plays a role in viral neuroinvasiveness. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Virology American Society For Microbiology

NS1′ of Flaviviruses in the Japanese Encephalitis Virus Serogroup Is a Product of Ribosomal Frameshifting and Plays a Role in Viral Neuroinvasiveness

NS1′ of Flaviviruses in the Japanese Encephalitis Virus Serogroup Is a Product of Ribosomal Frameshifting and Plays a Role in Viral Neuroinvasiveness

Journal of Virology , Volume 84 (3): 1641 – Feb 1, 2010

Abstract

NS1′ of Flaviviruses in the Japanese Encephalitis Virus Serogroup Is a Product of Ribosomal Frameshifting and Plays a Role in Viral Neuroinvasiveness ▿ Ezequiel Balmori Melian 1 , Edward Hinzman 1 , ‡ , Tomoko Nagasaki 1 , Andrew E. Firth 2 , Norma M. Wills 3 , Amanda S. Nouwens 5 , Bradley J. Blitvich 4 , Jason Leung 1 , † , Anneke Funk 1 , John F. Atkins 2 , 3 , Roy Hall 1 and Alexander A. Khromykh 1 , * 1 Centre for Infectious Disease Research, School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, QLD 4072, Australia 2 BioSciences Institute, University College Cork, Cork, Ireland 3 Department of Human Genetics, University of Utah, Salt Lake City, Utah 84112-5330 4 Iowa State University, Ames, Iowa 5 Australian Institute of Bioengineering and Nanotechnology, University of Queensland, Brisbane, QLD 4072, Australia ABSTRACT Flavivirus NS1 is a nonstructural protein involved in virus replication and regulation of the innate immune response. Interestingly, a larger NS1-related protein, NS1′, is often detected during infection with the members of the Japanese encephalitis virus serogroup of flaviviruses. However, how NS1′ is made and what role it performs in the viral life cycle have not been determined. Here we provide experimental evidence that NS1′ is the product of a −1 ribosomal frameshift event that occurs at a conserved slippery heptanucleotide motif located near the beginning of the NS2A gene and is stimulated by a downstream RNA pseudoknot structure. Using site-directed mutagenesis of these sequence elements in an infectious clone of the Kunjin subtype of West Nile virus, we demonstrate that NS1′ plays a role in viral neuroinvasiveness.

Loading next page...
 
/lp/american-society-for-microbiology/ns1-of-flaviviruses-in-the-japanese-encephalitis-virus-serogroup-is-a-YIgdIF3IYc

References (25)

Publisher
American Society For Microbiology
Copyright
Copyright © 2010 by the American society for Microbiology.
ISSN
0022-538X
eISSN
1098-5514
DOI
10.1128/JVI.01979-09
pmid
19906906
Publisher site
See Article on Publisher Site

Abstract

NS1′ of Flaviviruses in the Japanese Encephalitis Virus Serogroup Is a Product of Ribosomal Frameshifting and Plays a Role in Viral Neuroinvasiveness ▿ Ezequiel Balmori Melian 1 , Edward Hinzman 1 , ‡ , Tomoko Nagasaki 1 , Andrew E. Firth 2 , Norma M. Wills 3 , Amanda S. Nouwens 5 , Bradley J. Blitvich 4 , Jason Leung 1 , † , Anneke Funk 1 , John F. Atkins 2 , 3 , Roy Hall 1 and Alexander A. Khromykh 1 , * 1 Centre for Infectious Disease Research, School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, QLD 4072, Australia 2 BioSciences Institute, University College Cork, Cork, Ireland 3 Department of Human Genetics, University of Utah, Salt Lake City, Utah 84112-5330 4 Iowa State University, Ames, Iowa 5 Australian Institute of Bioengineering and Nanotechnology, University of Queensland, Brisbane, QLD 4072, Australia ABSTRACT Flavivirus NS1 is a nonstructural protein involved in virus replication and regulation of the innate immune response. Interestingly, a larger NS1-related protein, NS1′, is often detected during infection with the members of the Japanese encephalitis virus serogroup of flaviviruses. However, how NS1′ is made and what role it performs in the viral life cycle have not been determined. Here we provide experimental evidence that NS1′ is the product of a −1 ribosomal frameshift event that occurs at a conserved slippery heptanucleotide motif located near the beginning of the NS2A gene and is stimulated by a downstream RNA pseudoknot structure. Using site-directed mutagenesis of these sequence elements in an infectious clone of the Kunjin subtype of West Nile virus, we demonstrate that NS1′ plays a role in viral neuroinvasiveness.

Journal

Journal of VirologyAmerican Society For Microbiology

Published: Feb 1, 2010

There are no references for this article.