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New Design Strategy for Development of Specific Primer Sets for PCR-Based Detection of Chlorophyceae and Bacillariophyceae in Environmental Samples

New Design Strategy for Development of Specific Primer Sets for PCR-Based Detection of... New Design Strategy for Development of Specific Primer Sets for PCR-Based Detection of Chlorophyceae and Bacillariophyceae in Environmental Samples ▿ † Claire Valiente Moro * , Olivier Crouzet , Séréna Rasconi , Antoine Thouvenot , Gérard Coffe , Isabelle Batisson and Jacques Bohatier UMR 6023 Laboratoire Microorganismes: Génome et Environnement (CNRS-Université Blaise Pascal, Clermont-Ferrand II), 63177 Aubière, France ABSTRACT Studying aquatic microalgae is essential for monitoring biodiversity and water quality. We designed new sets of 18S rRNA PCR primers for Chlorophyceae and Bacillariophyceae by using the ARB software and implementing a virtual PCR program. The results of specificity analysis showed that most of the targeted algal families were identified and nontargeted organisms, such as fungi or ciliates, were excluded. These newly developed PCR primer sets were also able to amplify microalgal rRNA genes from environmental samples with accurate specificity. These tools could be of great interest for studying freshwater microalgal ecology and for developing bioindicators of the health status of aquatic environments. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied and Environmental Microbiology American Society For Microbiology

New Design Strategy for Development of Specific Primer Sets for PCR-Based Detection of Chlorophyceae and Bacillariophyceae in Environmental Samples

New Design Strategy for Development of Specific Primer Sets for PCR-Based Detection of Chlorophyceae and Bacillariophyceae in Environmental Samples

Applied and Environmental Microbiology , Volume 75 (17): 5729 – Sep 1, 2009

Abstract

New Design Strategy for Development of Specific Primer Sets for PCR-Based Detection of Chlorophyceae and Bacillariophyceae in Environmental Samples ▿ † Claire Valiente Moro * , Olivier Crouzet , Séréna Rasconi , Antoine Thouvenot , Gérard Coffe , Isabelle Batisson and Jacques Bohatier UMR 6023 Laboratoire Microorganismes: Génome et Environnement (CNRS-Université Blaise Pascal, Clermont-Ferrand II), 63177 Aubière, France ABSTRACT Studying aquatic microalgae is essential for monitoring biodiversity and water quality. We designed new sets of 18S rRNA PCR primers for Chlorophyceae and Bacillariophyceae by using the ARB software and implementing a virtual PCR program. The results of specificity analysis showed that most of the targeted algal families were identified and nontargeted organisms, such as fungi or ciliates, were excluded. These newly developed PCR primer sets were also able to amplify microalgal rRNA genes from environmental samples with accurate specificity. These tools could be of great interest for studying freshwater microalgal ecology and for developing bioindicators of the health status of aquatic environments.

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Publisher
American Society For Microbiology
Copyright
Copyright © 2009 by the American society for Microbiology.
ISSN
0099-2240
eISSN
1098-5336
DOI
10.1128/AEM.00509-09
pmid
19592531
Publisher site
See Article on Publisher Site

Abstract

New Design Strategy for Development of Specific Primer Sets for PCR-Based Detection of Chlorophyceae and Bacillariophyceae in Environmental Samples ▿ † Claire Valiente Moro * , Olivier Crouzet , Séréna Rasconi , Antoine Thouvenot , Gérard Coffe , Isabelle Batisson and Jacques Bohatier UMR 6023 Laboratoire Microorganismes: Génome et Environnement (CNRS-Université Blaise Pascal, Clermont-Ferrand II), 63177 Aubière, France ABSTRACT Studying aquatic microalgae is essential for monitoring biodiversity and water quality. We designed new sets of 18S rRNA PCR primers for Chlorophyceae and Bacillariophyceae by using the ARB software and implementing a virtual PCR program. The results of specificity analysis showed that most of the targeted algal families were identified and nontargeted organisms, such as fungi or ciliates, were excluded. These newly developed PCR primer sets were also able to amplify microalgal rRNA genes from environmental samples with accurate specificity. These tools could be of great interest for studying freshwater microalgal ecology and for developing bioindicators of the health status of aquatic environments.

Journal

Applied and Environmental MicrobiologyAmerican Society For Microbiology

Published: Sep 1, 2009

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