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New Chromosomal AmpC β-Lactamase in Enterobacter cloacae

New Chromosomal AmpC β-Lactamase in Enterobacter cloacae New Chromosomal AmpC β-Lactamase in Enterobacter cloacae T. Conceição , N. Faria , M. Pimentel , G. Soveral and A. Duarte * Faculty of Pharmacy University of Lisbon Av. Forças Armadas 1649-019 Lisbon, Portugal L. M. Lito , J. Melo Cristino and M. J. Salgado Faculty of Medicine Hospital de Santa Maria Lisbon, Portugal Several members of the Enterobacteriaceae , including Enterobacter spp., are naturally resistant to amoxicillin and cephalosporins. Enterobacter cloacae produces chromosomally encoded β-lactamases, also called cephalosporinases ( 1 ), and is a serious nosocomial pathogen, the third most prevalent bacterium isolated in intensive care settings ( 5 , 8 ). We report here the study of a new chromosomal AmpC β-lactamase produced by E. cloacae FFUL2En isolated from the blood culture of a patient hospitalized in a medicine ward of Hospital de Santa Maria, Lisbon, Portugal. The antibiogram revealed resistance to aminopenicillins, aztreonam, and broad-spectrum cephalosporins, except imipenem, aminoglycosides, and quinolones. By isoelectrofocusing, the sonicate extracts expressed a pI of 8.68, suggesting the presence of a presumed AmpC enzyme. A total DNA preparation from E. cloacae FFUL2En was used in PCR experiments with two sets of primers, TN5 (5′-CGTTTGTCAGGCACAGTCAAATCCA) and TN4 (5′-TTACTGTAGCGCGTCGAGGATATGG) and the internal http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Antimicrobial Agents and Chemotherapy American Society For Microbiology

New Chromosomal AmpC β-Lactamase in Enterobacter cloacae

New Chromosomal AmpC β-Lactamase in Enterobacter cloacae

Antimicrobial Agents and Chemotherapy , Volume 48 (4): 1437 – Apr 1, 2004

Abstract

New Chromosomal AmpC β-Lactamase in Enterobacter cloacae T. Conceição , N. Faria , M. Pimentel , G. Soveral and A. Duarte * Faculty of Pharmacy University of Lisbon Av. Forças Armadas 1649-019 Lisbon, Portugal L. M. Lito , J. Melo Cristino and M. J. Salgado Faculty of Medicine Hospital de Santa Maria Lisbon, Portugal Several members of the Enterobacteriaceae , including Enterobacter spp., are naturally resistant to amoxicillin and cephalosporins. Enterobacter cloacae produces chromosomally encoded β-lactamases, also called cephalosporinases ( 1 ), and is a serious nosocomial pathogen, the third most prevalent bacterium isolated in intensive care settings ( 5 , 8 ). We report here the study of a new chromosomal AmpC β-lactamase produced by E. cloacae FFUL2En isolated from the blood culture of a patient hospitalized in a medicine ward of Hospital de Santa Maria, Lisbon, Portugal. The antibiogram revealed resistance to aminopenicillins, aztreonam, and broad-spectrum cephalosporins, except imipenem, aminoglycosides, and quinolones. By isoelectrofocusing, the sonicate extracts expressed a pI of 8.68, suggesting the presence of a presumed AmpC enzyme. A total DNA preparation from E. cloacae FFUL2En was used in PCR experiments with two sets of primers, TN5 (5′-CGTTTGTCAGGCACAGTCAAATCCA) and TN4 (5′-TTACTGTAGCGCGTCGAGGATATGG) and the internal

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Publisher
American Society For Microbiology
Copyright
Copyright © 2004 by the American society for Microbiology.
ISSN
0066-4804
eISSN
1098-6596
DOI
10.1128/AAC.48.4.1437.2004
Publisher site
See Article on Publisher Site

Abstract

New Chromosomal AmpC β-Lactamase in Enterobacter cloacae T. Conceição , N. Faria , M. Pimentel , G. Soveral and A. Duarte * Faculty of Pharmacy University of Lisbon Av. Forças Armadas 1649-019 Lisbon, Portugal L. M. Lito , J. Melo Cristino and M. J. Salgado Faculty of Medicine Hospital de Santa Maria Lisbon, Portugal Several members of the Enterobacteriaceae , including Enterobacter spp., are naturally resistant to amoxicillin and cephalosporins. Enterobacter cloacae produces chromosomally encoded β-lactamases, also called cephalosporinases ( 1 ), and is a serious nosocomial pathogen, the third most prevalent bacterium isolated in intensive care settings ( 5 , 8 ). We report here the study of a new chromosomal AmpC β-lactamase produced by E. cloacae FFUL2En isolated from the blood culture of a patient hospitalized in a medicine ward of Hospital de Santa Maria, Lisbon, Portugal. The antibiogram revealed resistance to aminopenicillins, aztreonam, and broad-spectrum cephalosporins, except imipenem, aminoglycosides, and quinolones. By isoelectrofocusing, the sonicate extracts expressed a pI of 8.68, suggesting the presence of a presumed AmpC enzyme. A total DNA preparation from E. cloacae FFUL2En was used in PCR experiments with two sets of primers, TN5 (5′-CGTTTGTCAGGCACAGTCAAATCCA) and TN4 (5′-TTACTGTAGCGCGTCGAGGATATGG) and the internal

Journal

Antimicrobial Agents and ChemotherapyAmerican Society For Microbiology

Published: Apr 1, 2004

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