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Mutations in the Novel Membrane Protein Spinster Interfere with Programmed Cell Death and Cause Neural Degeneration inDrosophila melanogaster

Mutations in the Novel Membrane Protein Spinster Interfere with Programmed Cell Death and Cause... Mutations in the Novel Membrane Protein Spinster Interfere with Programmed Cell Death and Cause Neural Degeneration in Drosophila melanogaster Yoshiro Nakano 1 , 2 , 3 , † , Kazuko Fujitani 1 , 3 , Joyce Kurihara 2 , Janet Ragan 2 , Kazue Usui-Aoki 4 , Lori Shimoda 2 , Tamas Lukacsovich 1 , Keiko Suzuki 3 , 5 , Mariko Sezaki 3 , Yumiko Sano 3 , Ryu Ueda 3 , Wakae Awano 1 , Mizuho Kaneda 6 , Masato Umeda 6 , and Daisuke Yamamoto 1 , 3 , 4 , * ERATO Yamamoto Behavior Genes Project, Japan Science and Technology Corporation at Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo 194-8511, 1 Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo 194-8511, 3 Waseda University, School of Human Sciences and Advanced Research Institute for Science and Engineering, Tokorozawa, Saitama 359-1192, 4 Laboratory of Entomology, Tamagawa University, Machida, Tokyo 194-8610, 5 and The Tokyo Metropolitan Institute of Medical Science, Bunkyo-ku, Tokyo 113-8613, 6 Japan, and ERATO Yamamoto Behavior Genes Project, Japan Science and Technology Corporation at the Center for Conservation Biology Research and Training, University of Hawaii at Manoa, Honolulu, Hawaii 96822 2 ABSTRACT Mutations in the spin gene are characterized by an extraordinarily strong rejection behavior of female flies in response to male courtship. They are also accompanied by decreases in the viability, adult life span, and oviposition rate of the flies. In spin mutants, some oocytes and adult neural cells undergo degeneration, which is preceded by reductions in programmed cell death of nurse cells in ovaries and of neurons in the pupal nervous system, respectively. The central nervous system (CNS) of spin mutant flies accumulates autofluorescent lipopigments with characteristics similar to those of lipofuscin. The spin locus generates at least five different transcripts, with only two of these being able to rescue the spin behavioral phenotype; each encodes a protein with multiple membrane-spanning domains that are expressed in both the surface glial cells in the CNS and the follicle cells in the ovaries. Orthologs of the spin gene have also been identified in a number of species from nematodes to humans. Analysis of the spin mutant will give us new insights into neurodegenerative diseases and aging. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular and Cellular Biology American Society For Microbiology

Mutations in the Novel Membrane Protein Spinster Interfere with Programmed Cell Death and Cause Neural Degeneration inDrosophila melanogaster

Mutations in the Novel Membrane Protein Spinster Interfere with Programmed Cell Death and Cause Neural Degeneration inDrosophila melanogaster

Molecular and Cellular Biology , Volume 21 (11): 3775 – Jun 1, 2001

Abstract

Mutations in the Novel Membrane Protein Spinster Interfere with Programmed Cell Death and Cause Neural Degeneration in Drosophila melanogaster Yoshiro Nakano 1 , 2 , 3 , † , Kazuko Fujitani 1 , 3 , Joyce Kurihara 2 , Janet Ragan 2 , Kazue Usui-Aoki 4 , Lori Shimoda 2 , Tamas Lukacsovich 1 , Keiko Suzuki 3 , 5 , Mariko Sezaki 3 , Yumiko Sano 3 , Ryu Ueda 3 , Wakae Awano 1 , Mizuho Kaneda 6 , Masato Umeda 6 , and Daisuke Yamamoto 1 , 3 , 4 , * ERATO Yamamoto Behavior Genes Project, Japan Science and Technology Corporation at Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo 194-8511, 1 Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo 194-8511, 3 Waseda University, School of Human Sciences and Advanced Research Institute for Science and Engineering, Tokorozawa, Saitama 359-1192, 4 Laboratory of Entomology, Tamagawa University, Machida, Tokyo 194-8610, 5 and The Tokyo Metropolitan Institute of Medical Science, Bunkyo-ku, Tokyo 113-8613, 6 Japan, and ERATO Yamamoto Behavior Genes Project, Japan Science and Technology Corporation at the Center for Conservation Biology Research and Training, University of Hawaii at Manoa, Honolulu, Hawaii 96822 2 ABSTRACT Mutations in the spin gene are characterized by an extraordinarily strong rejection behavior of female flies in response to male courtship. They are also accompanied by decreases in the viability, adult life span, and oviposition rate of the flies. In spin mutants, some oocytes and adult neural cells undergo degeneration, which is preceded by reductions in programmed cell death of nurse cells in ovaries and of neurons in the pupal nervous system, respectively. The central nervous system (CNS) of spin mutant flies accumulates autofluorescent lipopigments with characteristics similar to those of lipofuscin. The spin locus generates at least five different transcripts, with only two of these being able to rescue the spin behavioral phenotype; each encodes a protein with multiple membrane-spanning domains that are expressed in both the surface glial cells in the CNS and the follicle cells in the ovaries. Orthologs of the spin gene have also been identified in a number of species from nematodes to humans. Analysis of the spin mutant will give us new insights into neurodegenerative diseases and aging.

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References (52)

Publisher
American Society For Microbiology
Copyright
Copyright © 2001 by the American society for Microbiology.
ISSN
0270-7306
eISSN
1098-5549
DOI
10.1128/MCB.21.11.3775-3788.2001
pmid
11340170
Publisher site
See Article on Publisher Site

Abstract

Mutations in the Novel Membrane Protein Spinster Interfere with Programmed Cell Death and Cause Neural Degeneration in Drosophila melanogaster Yoshiro Nakano 1 , 2 , 3 , † , Kazuko Fujitani 1 , 3 , Joyce Kurihara 2 , Janet Ragan 2 , Kazue Usui-Aoki 4 , Lori Shimoda 2 , Tamas Lukacsovich 1 , Keiko Suzuki 3 , 5 , Mariko Sezaki 3 , Yumiko Sano 3 , Ryu Ueda 3 , Wakae Awano 1 , Mizuho Kaneda 6 , Masato Umeda 6 , and Daisuke Yamamoto 1 , 3 , 4 , * ERATO Yamamoto Behavior Genes Project, Japan Science and Technology Corporation at Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo 194-8511, 1 Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo 194-8511, 3 Waseda University, School of Human Sciences and Advanced Research Institute for Science and Engineering, Tokorozawa, Saitama 359-1192, 4 Laboratory of Entomology, Tamagawa University, Machida, Tokyo 194-8610, 5 and The Tokyo Metropolitan Institute of Medical Science, Bunkyo-ku, Tokyo 113-8613, 6 Japan, and ERATO Yamamoto Behavior Genes Project, Japan Science and Technology Corporation at the Center for Conservation Biology Research and Training, University of Hawaii at Manoa, Honolulu, Hawaii 96822 2 ABSTRACT Mutations in the spin gene are characterized by an extraordinarily strong rejection behavior of female flies in response to male courtship. They are also accompanied by decreases in the viability, adult life span, and oviposition rate of the flies. In spin mutants, some oocytes and adult neural cells undergo degeneration, which is preceded by reductions in programmed cell death of nurse cells in ovaries and of neurons in the pupal nervous system, respectively. The central nervous system (CNS) of spin mutant flies accumulates autofluorescent lipopigments with characteristics similar to those of lipofuscin. The spin locus generates at least five different transcripts, with only two of these being able to rescue the spin behavioral phenotype; each encodes a protein with multiple membrane-spanning domains that are expressed in both the surface glial cells in the CNS and the follicle cells in the ovaries. Orthologs of the spin gene have also been identified in a number of species from nematodes to humans. Analysis of the spin mutant will give us new insights into neurodegenerative diseases and aging.

Journal

Molecular and Cellular BiologyAmerican Society For Microbiology

Published: Jun 1, 2001

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