Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Molecular Epidemiology of Penicillin-Susceptible Non-β-Lactam-Resistant Streptococcus pneumoniae Isolates from Greek Children

Molecular Epidemiology of Penicillin-Susceptible Non-β-Lactam-Resistant Streptococcus pneumoniae... Molecular Epidemiology of Penicillin-Susceptible Non-β-Lactam-Resistant Streptococcus pneumoniae Isolates from Greek Children D. Bogaert 1 , P. W. M. Hermans 1 , * , I. N. Grivea 2 , G. S. Katopodis 2 , T. J. Mitchell 3 , M. Sluijter 1 , R. de Groot 1 , N. G. Beratis 2 and G. A. Syrogiannopoulos 2 1 Department of Pediatrics, Sophia Children's Hospital, Erasmus MC Rotterdam, 3000 DR Rotterdam, The Netherlands 2 Department of Pediatrics, General University Hospital, University of Patras School of Medicine, 26 504 Rion, Patras, Greece 3 Division of Infection and Immunity, IBLS, University of Glasgow, Glasgow, United Kingdom ABSTRACT A total of 128 Streptococcus pneumoniae isolates that were susceptible to penicillin but resistant to non-β-lactam agents were isolated from young carriers in Greece and analyzed by antibiotic susceptibility testing, serotyping, restriction fragment end labeling (RFEL), and antibiotic resistance genotyping. The serotypes 6A/B (49%), 14 (14%), 19A/F (11%), 11A (9%), 23A/F (4%), 15B/C (2%), and 21 (2%) were most prevalent in this collection. Of the isolates, 65% were erythromycin resistant, while the remaining isolates were tetracycline and/or trimethoprim-sulfamethoxazole resistant. Fifty-nine distinct RFEL types were identified. Twenty different RFEL clusters, harboring 2 to 19 strains each, accounted for 76% of all strains. Confirmatory multilocus sequence typing analysis of the genetic clusters showed the presence of three international clones (Tennessee 23F -4, England 14 -9, and Greece 6B -22) representing 30% of the isolates. The erm (B) gene was present in 70% of the erythromycin-resistant isolates, whereas 18 and 8% contained the mef (A) and mef (E) genes, respectively. The pneumococci representing erm (B), erm (A), and mef genes belonged to distinct genetic clusters. In total, 45% of all isolates were tetracycline resistant. Ninety-six percent of these isolates contained the tet (M) gene. In conclusion, penicillin-susceptible pneumococci resistant to non-β-lactams are a genetically heterogeneous group displaying a variety of genotypes, resistance markers, and serotypes. This suggests that multiple genetic events lead to non-β-lactam-resistant pneumococci in Greece. Importantly, most of these genotypes are capable of disseminating within the community. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Clinical Microbiology American Society For Microbiology

Molecular Epidemiology of Penicillin-Susceptible Non-β-Lactam-Resistant Streptococcus pneumoniae Isolates from Greek Children

Molecular Epidemiology of Penicillin-Susceptible Non-β-Lactam-Resistant Streptococcus pneumoniae Isolates from Greek Children

Journal of Clinical Microbiology , Volume 41 (12): 5633 – Dec 1, 2003

Abstract

Molecular Epidemiology of Penicillin-Susceptible Non-β-Lactam-Resistant Streptococcus pneumoniae Isolates from Greek Children D. Bogaert 1 , P. W. M. Hermans 1 , * , I. N. Grivea 2 , G. S. Katopodis 2 , T. J. Mitchell 3 , M. Sluijter 1 , R. de Groot 1 , N. G. Beratis 2 and G. A. Syrogiannopoulos 2 1 Department of Pediatrics, Sophia Children's Hospital, Erasmus MC Rotterdam, 3000 DR Rotterdam, The Netherlands 2 Department of Pediatrics, General University Hospital, University of Patras School of Medicine, 26 504 Rion, Patras, Greece 3 Division of Infection and Immunity, IBLS, University of Glasgow, Glasgow, United Kingdom ABSTRACT A total of 128 Streptococcus pneumoniae isolates that were susceptible to penicillin but resistant to non-β-lactam agents were isolated from young carriers in Greece and analyzed by antibiotic susceptibility testing, serotyping, restriction fragment end labeling (RFEL), and antibiotic resistance genotyping. The serotypes 6A/B (49%), 14 (14%), 19A/F (11%), 11A (9%), 23A/F (4%), 15B/C (2%), and 21 (2%) were most prevalent in this collection. Of the isolates, 65% were erythromycin resistant, while the remaining isolates were tetracycline and/or trimethoprim-sulfamethoxazole resistant. Fifty-nine distinct RFEL types were identified. Twenty different RFEL clusters, harboring 2 to 19 strains each, accounted for 76% of all strains. Confirmatory multilocus sequence typing analysis of the genetic clusters showed the presence of three international clones (Tennessee 23F -4, England 14 -9, and Greece 6B -22) representing 30% of the isolates. The erm (B) gene was present in 70% of the erythromycin-resistant isolates, whereas 18 and 8% contained the mef (A) and mef (E) genes, respectively. The pneumococci representing erm (B), erm (A), and mef genes belonged to distinct genetic clusters. In total, 45% of all isolates were tetracycline resistant. Ninety-six percent of these isolates contained the tet (M) gene. In conclusion, penicillin-susceptible pneumococci resistant to non-β-lactams are a genetically heterogeneous group displaying a variety of genotypes, resistance markers, and serotypes. This suggests that multiple genetic events lead to non-β-lactam-resistant pneumococci in Greece. Importantly, most of these genotypes are capable of disseminating within the community.

Loading next page...
 
/lp/american-society-for-microbiology/molecular-epidemiology-of-penicillin-susceptible-non-lactam-resistant-P0ZRRrd5c6

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
American Society For Microbiology
Copyright
Copyright © 2003 by the American society for Microbiology.
ISSN
0095-1137
eISSN
1098-660X
DOI
10.1128/JCM.41.12.5633-5639.2003
Publisher site
See Article on Publisher Site

Abstract

Molecular Epidemiology of Penicillin-Susceptible Non-β-Lactam-Resistant Streptococcus pneumoniae Isolates from Greek Children D. Bogaert 1 , P. W. M. Hermans 1 , * , I. N. Grivea 2 , G. S. Katopodis 2 , T. J. Mitchell 3 , M. Sluijter 1 , R. de Groot 1 , N. G. Beratis 2 and G. A. Syrogiannopoulos 2 1 Department of Pediatrics, Sophia Children's Hospital, Erasmus MC Rotterdam, 3000 DR Rotterdam, The Netherlands 2 Department of Pediatrics, General University Hospital, University of Patras School of Medicine, 26 504 Rion, Patras, Greece 3 Division of Infection and Immunity, IBLS, University of Glasgow, Glasgow, United Kingdom ABSTRACT A total of 128 Streptococcus pneumoniae isolates that were susceptible to penicillin but resistant to non-β-lactam agents were isolated from young carriers in Greece and analyzed by antibiotic susceptibility testing, serotyping, restriction fragment end labeling (RFEL), and antibiotic resistance genotyping. The serotypes 6A/B (49%), 14 (14%), 19A/F (11%), 11A (9%), 23A/F (4%), 15B/C (2%), and 21 (2%) were most prevalent in this collection. Of the isolates, 65% were erythromycin resistant, while the remaining isolates were tetracycline and/or trimethoprim-sulfamethoxazole resistant. Fifty-nine distinct RFEL types were identified. Twenty different RFEL clusters, harboring 2 to 19 strains each, accounted for 76% of all strains. Confirmatory multilocus sequence typing analysis of the genetic clusters showed the presence of three international clones (Tennessee 23F -4, England 14 -9, and Greece 6B -22) representing 30% of the isolates. The erm (B) gene was present in 70% of the erythromycin-resistant isolates, whereas 18 and 8% contained the mef (A) and mef (E) genes, respectively. The pneumococci representing erm (B), erm (A), and mef genes belonged to distinct genetic clusters. In total, 45% of all isolates were tetracycline resistant. Ninety-six percent of these isolates contained the tet (M) gene. In conclusion, penicillin-susceptible pneumococci resistant to non-β-lactams are a genetically heterogeneous group displaying a variety of genotypes, resistance markers, and serotypes. This suggests that multiple genetic events lead to non-β-lactam-resistant pneumococci in Greece. Importantly, most of these genotypes are capable of disseminating within the community.

Journal

Journal of Clinical MicrobiologyAmerican Society For Microbiology

Published: Dec 1, 2003

There are no references for this article.