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Enzymatic Production of l-Alanine by Pseudomonas dacunhae

Enzymatic Production of l-Alanine by Pseudomonas dacunhae Enzymatic Production of l -Alanine by Pseudomonas dacunhae Ichiro Chibata , Toshio Kakimoto and Jyoji Kato Osaka Research Laboratory, Tanabe Seiyaku Company, Ltd., Higashi-yodogawa-ku, Osaka, Japan ABSTRACT To establish an advantageous method for the production of l -alanine, a procedure was studied for converting l -aspartic acid to l -alanine by microbial l -aspartic β-decarboxylase. A number of organisms were screened to test their ability to form and accumulate alanine from aspartic acid. Pseudomonas dacunhae was selected as the most advantageous organism. With this organism, enzyme activity as high as 3,910 μliters of CO 2 per hr per ml of medium could be produced by shaking the culture at 30 C in the medium containing ammonium fumarate, sodium fumarate, corn steep liquor, peptone, and inorganic salts. For the enzymatic conversion of l -aspartic acid to l -alanine, the culture broth was employed as the enzyme source. A large amount of l -aspartic acid (as much as 40% of the broth) was converted stoichiometrically to alanine in 72 hr at 37 C. Furthermore, appropriate addition of a surface-active agent to the reaction mixture was found to be highly effective in shortening the time required for the conversion. Accumulated l -alanine was readily isolated in pure form by ordinary procedures with ion-exchange resins. Yields of isolated l -alanine of over 90% from l -aspartic acid were easily attainable. Copyright © 1965 American Society for Microbiology CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? « Previous | Next Article » Table of Contents This Article Appl. Environ. Microbiol. September 1965 vol. 13 no. 5 638-645 » Abstract PDF Classifications ARTICLE Services Email this article to a colleague Similar articles in ASM journals Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of AEM Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Load citing article information Citing articles via Web of Science Citing articles via Google Scholar Google Scholar Articles by Chibata, I. Articles by Kato, J. Search for related content PubMed PubMed citation Articles by Chibata, I. Articles by Kato, J. Related Content Load related web page information Social Bookmarking CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? current issue December 2011, volume 77, issue 23 Alert me to new issues of AEM About AEM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy AEM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0099-2240 Online ISSN: 1098-5336 Copyright © 2011 by the American Society for Microbiology. For an alternate route to AEM .asm.org, visit: http://intl- AEM .asm.org | More Info» var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www."); document.write(unescape("%3Cscript src='" + gaJsHost + "google-analytics.com/ga.js' type='text/javascript'%3E%3C/script%3E")); var pageTracker = _gat._getTracker("UA-5821458-4"); pageTracker._trackPageview(); http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied and Environmental Microbiology American Society For Microbiology

Enzymatic Production of l-Alanine by Pseudomonas dacunhae

Enzymatic Production of l-Alanine by Pseudomonas dacunhae

Applied and Environmental Microbiology , Volume 13 (5): 638 – Sep 1, 1965

Abstract

Enzymatic Production of l -Alanine by Pseudomonas dacunhae Ichiro Chibata , Toshio Kakimoto and Jyoji Kato Osaka Research Laboratory, Tanabe Seiyaku Company, Ltd., Higashi-yodogawa-ku, Osaka, Japan ABSTRACT To establish an advantageous method for the production of l -alanine, a procedure was studied for converting l -aspartic acid to l -alanine by microbial l -aspartic β-decarboxylase. A number of organisms were screened to test their ability to form and accumulate alanine from aspartic acid. Pseudomonas dacunhae was selected as the most advantageous organism. With this organism, enzyme activity as high as 3,910 μliters of CO 2 per hr per ml of medium could be produced by shaking the culture at 30 C in the medium containing ammonium fumarate, sodium fumarate, corn steep liquor, peptone, and inorganic salts. For the enzymatic conversion of l -aspartic acid to l -alanine, the culture broth was employed as the enzyme source. A large amount of l -aspartic acid (as much as 40% of the broth) was converted stoichiometrically to alanine in 72 hr at 37 C. Furthermore, appropriate addition of a surface-active agent to the reaction mixture was found to be highly effective in shortening the time required for the conversion. Accumulated l -alanine was readily isolated in pure form by ordinary procedures with ion-exchange resins. Yields of isolated l -alanine of over 90% from l -aspartic acid were easily attainable. Copyright © 1965 American Society for Microbiology CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? « Previous | Next Article » Table of Contents This Article Appl. Environ. Microbiol. September 1965 vol. 13 no. 5 638-645 » Abstract PDF Classifications ARTICLE Services Email this article to a colleague Similar articles in ASM journals Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of AEM Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Load citing article information Citing articles via Web of Science Citing articles via Google Scholar Google Scholar Articles by Chibata, I. Articles by Kato, J. Search for related content PubMed PubMed citation Articles by Chibata, I. Articles by Kato, J. Related Content Load related web page information Social Bookmarking CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? current issue December 2011, volume 77, issue 23 Alert me to new issues of AEM About AEM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy AEM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0099-2240 Online ISSN: 1098-5336 Copyright © 2011 by the American Society for Microbiology. For an alternate route to AEM .asm.org, visit: http://intl- AEM .asm.org | More Info» var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www."); document.write(unescape("%3Cscript src='" + gaJsHost + "google-analytics.com/ga.js' type='text/javascript'%3E%3C/script%3E")); var pageTracker = _gat._getTracker("UA-5821458-4"); pageTracker._trackPageview();

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Publisher
American Society For Microbiology
Copyright
Copyright © 1965 by the American society for Microbiology.
ISSN
0099-2240
eISSN
1098-5336
Publisher site
See Article on Publisher Site

Abstract

Enzymatic Production of l -Alanine by Pseudomonas dacunhae Ichiro Chibata , Toshio Kakimoto and Jyoji Kato Osaka Research Laboratory, Tanabe Seiyaku Company, Ltd., Higashi-yodogawa-ku, Osaka, Japan ABSTRACT To establish an advantageous method for the production of l -alanine, a procedure was studied for converting l -aspartic acid to l -alanine by microbial l -aspartic β-decarboxylase. A number of organisms were screened to test their ability to form and accumulate alanine from aspartic acid. Pseudomonas dacunhae was selected as the most advantageous organism. With this organism, enzyme activity as high as 3,910 μliters of CO 2 per hr per ml of medium could be produced by shaking the culture at 30 C in the medium containing ammonium fumarate, sodium fumarate, corn steep liquor, peptone, and inorganic salts. For the enzymatic conversion of l -aspartic acid to l -alanine, the culture broth was employed as the enzyme source. A large amount of l -aspartic acid (as much as 40% of the broth) was converted stoichiometrically to alanine in 72 hr at 37 C. Furthermore, appropriate addition of a surface-active agent to the reaction mixture was found to be highly effective in shortening the time required for the conversion. Accumulated l -alanine was readily isolated in pure form by ordinary procedures with ion-exchange resins. Yields of isolated l -alanine of over 90% from l -aspartic acid were easily attainable. Copyright © 1965 American Society for Microbiology CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? « Previous | Next Article » Table of Contents This Article Appl. Environ. Microbiol. September 1965 vol. 13 no. 5 638-645 » Abstract PDF Classifications ARTICLE Services Email this article to a colleague Similar articles in ASM journals Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of AEM Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Load citing article information Citing articles via Web of Science Citing articles via Google Scholar Google Scholar Articles by Chibata, I. Articles by Kato, J. Search for related content PubMed PubMed citation Articles by Chibata, I. Articles by Kato, J. Related Content Load related web page information Social Bookmarking CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? current issue December 2011, volume 77, issue 23 Alert me to new issues of AEM About AEM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy AEM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0099-2240 Online ISSN: 1098-5336 Copyright © 2011 by the American Society for Microbiology. For an alternate route to AEM .asm.org, visit: http://intl- AEM .asm.org | More Info» var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www."); document.write(unescape("%3Cscript src='" + gaJsHost + "google-analytics.com/ga.js' type='text/javascript'%3E%3C/script%3E")); var pageTracker = _gat._getTracker("UA-5821458-4"); pageTracker._trackPageview();

Journal

Applied and Environmental MicrobiologyAmerican Society For Microbiology

Published: Sep 1, 1965

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