ENZYMATIC BREAKDOWN OF CITRATE IN THE ABSENCE OF COENZYME A
Abstract
CONTENT ALERTS Receive: RSS Feeds, eTOCs, free email alerts (when new articles cite this article), more» Information about commercial reprint orders: http://jb.asm.org/site/misc/reprints.xhtml To subscribe to to another ASM Journal go to: http://journals.asm.org/site/subscriptions/ ROBERT W. WHEAT,' DONALD T. 0. WONG, AND SAMUEL J. AJL Department of Bacteriology, Communicable Diseases Division, Army Medical Service Graduate School, Washington, D. C. Received for publication November 23, 1953 and cell-free extracts were prepared by grinding with "aluimina 303" powder essentially as described by McIlwain (1948). Conventional Warburg manometric techniques were used for CO2 evolution studies. For anaerobic experiments the vessels were filled with N2 freed from residual 02 by passing the gas through a series of absorption towers containing alkaline pyrogallol. To remove coenzyme A, extracts were treated with the anion exchange resin "dowex-l" according to the procedure described by Chantrenne and Lipmann (1950). Citric acid was determined by the method of Natelson et al. (1948), a-ketoglutarate by the method of Koepsell and Sharpe (1952), pyruvate by the toluene extraction method of Friedemann and Haugen (1943), and succinate by the conventional succinoxidase procedure. All radioassays were made with a conventional end window Geiger-Muller tube counter connected to an autoscaler. Neutralized solutions were pipetted