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Dissemination of CTX-M-Type β-Lactamases among Clinical Isolates of Enterobacteriaceae in Paris, France

Dissemination of CTX-M-Type β-Lactamases among Clinical Isolates of Enterobacteriaceae in Paris,... Dissemination of CTX-M-Type β-Lactamases among Clinical Isolates of Enterobacteriaceae in Paris, France C. Eckert 1 , V. Gautier 1 , M. Saladin-Allard 2 , N. Hidri 3 , C. Verdet 4 , Z. Ould-Hocine 4 , G. Barnaud 4 , F. Delisle 4 , A. Rossier 4 , T. Lambert 5 , A. Philippon 6 and G. Arlet 1 , 4 , * 1 Laboratoire de Bactériologie, UPRES EA 2392, UFR Saint-Antoine, Université Paris VI 4 Service de Bactériologie-Hygiène, Hôpital Tenon, AP-HP 5 Service de Bactériologie, Hôpital Saint-Michel 6 Service de Bactériologie, CHU Cochin, AP-HP, Paris 2 Service de Biologie, Hôpital Robert Ballanger, Aulnay-sous-bois 3 Service de Microbiologie, Hôpital Louis Mourier, AP-HP, Colombes, France ABSTRACT We analyzed 19 clinical isolates of the family Enterobacteriaceae (16 Escherichia coli isolates and 3 Klebsiella pneumoniae isolates) collected from four different hospitals in Paris, France, from 2000 to 2002. These strains had a particular extended-spectrum cephalosporin resistance profile characterized by a higher level of resistance to cefotaxime and aztreonam than to ceftazidime. The bla CTX-M genes encoding these β-lactamases were involved in this resistance, with a predominance of bla CTX-M-15 . Ten of the 19 isolates produced both TEM-1- and CTX-M-type enzymes. One strain ( E. coli TN13) expressed CMY-2, TEM-1, and CTX-M-14. bla CTX-M genes were found on large plasmids. In 15 cases the same insertion sequence, IS Ecp1 , was located upstream of the 5′ end of the bla CTX-M gene. In one case we identified an insertion sequence designated IS 26 . Examination of the other three bla CTX-M genes by cloning, sequencing, and PCR analysis revealed the presence of a complex sul1 -type integron that includes open reading frame ORF513, which carries the bla gene and the surrounding DNA. Five isolates had the same plasmid DNA fingerprint, suggesting clonal dissemination of CTX-M-15-producing strains in the Paris area. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Antimicrobial Agents and Chemotherapy American Society For Microbiology

Dissemination of CTX-M-Type β-Lactamases among Clinical Isolates of Enterobacteriaceae in Paris, France

Dissemination of CTX-M-Type β-Lactamases among Clinical Isolates of Enterobacteriaceae in Paris, France

Antimicrobial Agents and Chemotherapy , Volume 48 (4): 1249 – Apr 1, 2004

Abstract

Dissemination of CTX-M-Type β-Lactamases among Clinical Isolates of Enterobacteriaceae in Paris, France C. Eckert 1 , V. Gautier 1 , M. Saladin-Allard 2 , N. Hidri 3 , C. Verdet 4 , Z. Ould-Hocine 4 , G. Barnaud 4 , F. Delisle 4 , A. Rossier 4 , T. Lambert 5 , A. Philippon 6 and G. Arlet 1 , 4 , * 1 Laboratoire de Bactériologie, UPRES EA 2392, UFR Saint-Antoine, Université Paris VI 4 Service de Bactériologie-Hygiène, Hôpital Tenon, AP-HP 5 Service de Bactériologie, Hôpital Saint-Michel 6 Service de Bactériologie, CHU Cochin, AP-HP, Paris 2 Service de Biologie, Hôpital Robert Ballanger, Aulnay-sous-bois 3 Service de Microbiologie, Hôpital Louis Mourier, AP-HP, Colombes, France ABSTRACT We analyzed 19 clinical isolates of the family Enterobacteriaceae (16 Escherichia coli isolates and 3 Klebsiella pneumoniae isolates) collected from four different hospitals in Paris, France, from 2000 to 2002. These strains had a particular extended-spectrum cephalosporin resistance profile characterized by a higher level of resistance to cefotaxime and aztreonam than to ceftazidime. The bla CTX-M genes encoding these β-lactamases were involved in this resistance, with a predominance of bla CTX-M-15 . Ten of the 19 isolates produced both TEM-1- and CTX-M-type enzymes. One strain ( E. coli TN13) expressed CMY-2, TEM-1, and CTX-M-14. bla CTX-M genes were found on large plasmids. In 15 cases the same insertion sequence, IS Ecp1 , was located upstream of the 5′ end of the bla CTX-M gene. In one case we identified an insertion sequence designated IS 26 . Examination of the other three bla CTX-M genes by cloning, sequencing, and PCR analysis revealed the presence of a complex sul1 -type integron that includes open reading frame ORF513, which carries the bla gene and the surrounding DNA. Five isolates had the same plasmid DNA fingerprint, suggesting clonal dissemination of CTX-M-15-producing strains in the Paris area.

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References (43)

Publisher
American Society For Microbiology
Copyright
Copyright © 2004 by the American society for Microbiology.
ISSN
0066-4804
eISSN
1098-6596
DOI
10.1128/AAC.48.4.1249-1255.2004
Publisher site
See Article on Publisher Site

Abstract

Dissemination of CTX-M-Type β-Lactamases among Clinical Isolates of Enterobacteriaceae in Paris, France C. Eckert 1 , V. Gautier 1 , M. Saladin-Allard 2 , N. Hidri 3 , C. Verdet 4 , Z. Ould-Hocine 4 , G. Barnaud 4 , F. Delisle 4 , A. Rossier 4 , T. Lambert 5 , A. Philippon 6 and G. Arlet 1 , 4 , * 1 Laboratoire de Bactériologie, UPRES EA 2392, UFR Saint-Antoine, Université Paris VI 4 Service de Bactériologie-Hygiène, Hôpital Tenon, AP-HP 5 Service de Bactériologie, Hôpital Saint-Michel 6 Service de Bactériologie, CHU Cochin, AP-HP, Paris 2 Service de Biologie, Hôpital Robert Ballanger, Aulnay-sous-bois 3 Service de Microbiologie, Hôpital Louis Mourier, AP-HP, Colombes, France ABSTRACT We analyzed 19 clinical isolates of the family Enterobacteriaceae (16 Escherichia coli isolates and 3 Klebsiella pneumoniae isolates) collected from four different hospitals in Paris, France, from 2000 to 2002. These strains had a particular extended-spectrum cephalosporin resistance profile characterized by a higher level of resistance to cefotaxime and aztreonam than to ceftazidime. The bla CTX-M genes encoding these β-lactamases were involved in this resistance, with a predominance of bla CTX-M-15 . Ten of the 19 isolates produced both TEM-1- and CTX-M-type enzymes. One strain ( E. coli TN13) expressed CMY-2, TEM-1, and CTX-M-14. bla CTX-M genes were found on large plasmids. In 15 cases the same insertion sequence, IS Ecp1 , was located upstream of the 5′ end of the bla CTX-M gene. In one case we identified an insertion sequence designated IS 26 . Examination of the other three bla CTX-M genes by cloning, sequencing, and PCR analysis revealed the presence of a complex sul1 -type integron that includes open reading frame ORF513, which carries the bla gene and the surrounding DNA. Five isolates had the same plasmid DNA fingerprint, suggesting clonal dissemination of CTX-M-15-producing strains in the Paris area.

Journal

Antimicrobial Agents and ChemotherapyAmerican Society For Microbiology

Published: Apr 1, 2004

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