Development and Applications of a Bovine Coronavirus Antigen Detection Enzyme-Linked Immunosorbent Assay
Abstract
Development and Applications of a Bovine Coronavirus Antigen Detection Enzyme-Linked Immunosorbent Assay † S. L. Schoenthaler ‡ and S. Kapil * Department of Diagnostic Medicine-Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas 66506-5606 ABSTRACT We developed a monoclonal antibody-based, antigen capture sandwich enzyme-linked immunosorbent assay (ELISA) for bovine coronavirus. We compared the ELISA with electron microscopy and the hemagglutination test and found a close correlation between them. The sensitivity of the ELISA was 10 4 bovine coronavirus particles per ml of 10% fecal suspension. Compared with electron microscopy, bovine coronavirus ELISA had 96% specificity.