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Development and Applications of a Bovine Coronavirus Antigen Detection Enzyme-Linked Immunosorbent Assay

Development and Applications of a Bovine Coronavirus Antigen Detection Enzyme-Linked... Development and Applications of a Bovine Coronavirus Antigen Detection Enzyme-Linked Immunosorbent Assay † S. L. Schoenthaler ‡ and S. Kapil * Department of Diagnostic Medicine-Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas 66506-5606 ABSTRACT We developed a monoclonal antibody-based, antigen capture sandwich enzyme-linked immunosorbent assay (ELISA) for bovine coronavirus. We compared the ELISA with electron microscopy and the hemagglutination test and found a close correlation between them. The sensitivity of the ELISA was 10 4 bovine coronavirus particles per ml of 10% fecal suspension. Compared with electron microscopy, bovine coronavirus ELISA had 96% specificity. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical and Vaccine Immunology American Society For Microbiology

Development and Applications of a Bovine Coronavirus Antigen Detection Enzyme-Linked Immunosorbent Assay

Clinical and Vaccine Immunology , Volume 6 (1): 130 – Jan 1, 1999

Development and Applications of a Bovine Coronavirus Antigen Detection Enzyme-Linked Immunosorbent Assay

Clinical and Vaccine Immunology , Volume 6 (1): 130 – Jan 1, 1999

Abstract

Development and Applications of a Bovine Coronavirus Antigen Detection Enzyme-Linked Immunosorbent Assay † S. L. Schoenthaler ‡ and S. Kapil * Department of Diagnostic Medicine-Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas 66506-5606 ABSTRACT We developed a monoclonal antibody-based, antigen capture sandwich enzyme-linked immunosorbent assay (ELISA) for bovine coronavirus. We compared the ELISA with electron microscopy and the hemagglutination test and found a close correlation between them. The sensitivity of the ELISA was 10 4 bovine coronavirus particles per ml of 10% fecal suspension. Compared with electron microscopy, bovine coronavirus ELISA had 96% specificity.

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Publisher
American Society For Microbiology
Copyright
Copyright © 1999 by the American society for Microbiology.
ISSN
1556-6811
eISSN
1556-679X
Publisher site
See Article on Publisher Site

Abstract

Development and Applications of a Bovine Coronavirus Antigen Detection Enzyme-Linked Immunosorbent Assay † S. L. Schoenthaler ‡ and S. Kapil * Department of Diagnostic Medicine-Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas 66506-5606 ABSTRACT We developed a monoclonal antibody-based, antigen capture sandwich enzyme-linked immunosorbent assay (ELISA) for bovine coronavirus. We compared the ELISA with electron microscopy and the hemagglutination test and found a close correlation between them. The sensitivity of the ELISA was 10 4 bovine coronavirus particles per ml of 10% fecal suspension. Compared with electron microscopy, bovine coronavirus ELISA had 96% specificity.

Journal

Clinical and Vaccine ImmunologyAmerican Society For Microbiology

Published: Jan 1, 1999

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