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Cyanide Formation by Chromobacterium violaceum

Cyanide Formation by Chromobacterium violaceum Cyanide Formation by Chromobacterium violaceum ABSTRACT Michaels, Ruth (Columbia University, New York, N.Y.), and W. A. Corpe . Cyanide formation by Chromobacterium violaceum . J. Bacteriol. 89: 106–112. 1965.—The formation of cyanide by a Chromobacterium violaceum strain was studied with growing cultures and with nonproliferating cells grown in complex and chemically defined media. Most of the cyanide was produced during the log-phase growth of the organism, and accumulated in the culture supernatant fluid. A synergistic effect of glycine and methionine on cyanide formation in a chemically defined medium was observed, and the amount of cyanide formed was found to be dependent on the concentrations of the two substances. Cyanide formation by nonproliferating cells was stimulated by preincubation with glycine and methionine. Cyanide formation by adapted cells in the presence of glycine and methionine was stimulated by succinate, malate, or fumarate, and depressed by azide and 2,4-dinitrophenol. Methionine could be replaced by betaine, dimethylglycine, and choline. Ruth Michaels 1 and W. A. Corpe a Department of Botany, Columbia University, New York, New York http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Bacteriology American Society For Microbiology

Cyanide Formation by Chromobacterium violaceum

Journal of Bacteriology , Volume 89 (1): 106 – Jan 1, 1965

Cyanide Formation by Chromobacterium violaceum

Journal of Bacteriology , Volume 89 (1): 106 – Jan 1, 1965

Abstract

Cyanide Formation by Chromobacterium violaceum ABSTRACT Michaels, Ruth (Columbia University, New York, N.Y.), and W. A. Corpe . Cyanide formation by Chromobacterium violaceum . J. Bacteriol. 89: 106–112. 1965.—The formation of cyanide by a Chromobacterium violaceum strain was studied with growing cultures and with nonproliferating cells grown in complex and chemically defined media. Most of the cyanide was produced during the log-phase growth of the organism, and accumulated in the culture supernatant fluid. A synergistic effect of glycine and methionine on cyanide formation in a chemically defined medium was observed, and the amount of cyanide formed was found to be dependent on the concentrations of the two substances. Cyanide formation by nonproliferating cells was stimulated by preincubation with glycine and methionine. Cyanide formation by adapted cells in the presence of glycine and methionine was stimulated by succinate, malate, or fumarate, and depressed by azide and 2,4-dinitrophenol. Methionine could be replaced by betaine, dimethylglycine, and choline. Ruth Michaels 1 and W. A. Corpe a Department of Botany, Columbia University, New York, New York

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Publisher
American Society For Microbiology
Copyright
Copyright © 1965 by the American society for Microbiology.
ISSN
0021-9193
eISSN
1098-5530
Publisher site
See Article on Publisher Site

Abstract

Cyanide Formation by Chromobacterium violaceum ABSTRACT Michaels, Ruth (Columbia University, New York, N.Y.), and W. A. Corpe . Cyanide formation by Chromobacterium violaceum . J. Bacteriol. 89: 106–112. 1965.—The formation of cyanide by a Chromobacterium violaceum strain was studied with growing cultures and with nonproliferating cells grown in complex and chemically defined media. Most of the cyanide was produced during the log-phase growth of the organism, and accumulated in the culture supernatant fluid. A synergistic effect of glycine and methionine on cyanide formation in a chemically defined medium was observed, and the amount of cyanide formed was found to be dependent on the concentrations of the two substances. Cyanide formation by nonproliferating cells was stimulated by preincubation with glycine and methionine. Cyanide formation by adapted cells in the presence of glycine and methionine was stimulated by succinate, malate, or fumarate, and depressed by azide and 2,4-dinitrophenol. Methionine could be replaced by betaine, dimethylglycine, and choline. Ruth Michaels 1 and W. A. Corpe a Department of Botany, Columbia University, New York, New York

Journal

Journal of BacteriologyAmerican Society For Microbiology

Published: Jan 1, 1965

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