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Characterization of the Escherichia coli AaeAB Efflux Pump: a Metabolic Relief Valve?

Characterization of the Escherichia coli AaeAB Efflux Pump: a Metabolic Relief Valve? Characterization of the Escherichia coli AaeAB Efflux Pump: a Metabolic Relief Valve? Tina K. Van Dyk * , Lori J. Templeton , Keith A. Cantera , Pamela L. Sharpe , and F. Sima Sariaslani DuPont Company, Central Research and Development Department, Wilmington, Delaware ABSTRACT Treatment of Escherichia coli with p -hydroxybenzoic acid (pHBA) resulted in upregulation of yhcP , encoding a protein of the putative efflux protein family. Also upregulated were the adjacent genes yhcQ , encoding a protein of the membrane fusion protein family, and yhcR , encoding a small protein without a known or suggested function. The function of the upstream, divergently transcribed gene yhcS , encoding a regulatory protein of the LysR family, in regulating expression of yhcRQP was shown. Furthermore, it was demonstrated that several aromatic carboxylic acid compounds serve as inducers of yhcRQP expression. The efflux function encoded by yhcP was proven by the hypersensitivity to pHBA of a yhcP mutant strain. A yhcS mutant strain was also hypersensitive to pHBA. Expression of yhcQ and yhcP was necessary and sufficient for suppression of the pHBA hypersensitivity of the yhcS mutant. Only a few aromatic carboxylic acids of hundreds of diverse compounds tested were defined as substrates of the YhcQP efflux pump. Thus, we propose renaming yhcS , yhcR , yhcQ , and yhcP , to reflect their role in aromatic carboxylic acid efflux, to aaeR , aaeX , aaeA , and aaeB , respectively. The role of pHBA in normal E. coli metabolism and the highly regulated expression of the AaeAB efflux system suggests that the physiological role may be as a “metabolic relief valve” to alleviate toxic effects of imbalanced metabolism. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Bacteriology American Society For Microbiology

Characterization of the Escherichia coli AaeAB Efflux Pump: a Metabolic Relief Valve?

Characterization of the Escherichia coli AaeAB Efflux Pump: a Metabolic Relief Valve?

Journal of Bacteriology , Volume 186 (21): 7196 – Nov 1, 2004

Abstract

Characterization of the Escherichia coli AaeAB Efflux Pump: a Metabolic Relief Valve? Tina K. Van Dyk * , Lori J. Templeton , Keith A. Cantera , Pamela L. Sharpe , and F. Sima Sariaslani DuPont Company, Central Research and Development Department, Wilmington, Delaware ABSTRACT Treatment of Escherichia coli with p -hydroxybenzoic acid (pHBA) resulted in upregulation of yhcP , encoding a protein of the putative efflux protein family. Also upregulated were the adjacent genes yhcQ , encoding a protein of the membrane fusion protein family, and yhcR , encoding a small protein without a known or suggested function. The function of the upstream, divergently transcribed gene yhcS , encoding a regulatory protein of the LysR family, in regulating expression of yhcRQP was shown. Furthermore, it was demonstrated that several aromatic carboxylic acid compounds serve as inducers of yhcRQP expression. The efflux function encoded by yhcP was proven by the hypersensitivity to pHBA of a yhcP mutant strain. A yhcS mutant strain was also hypersensitive to pHBA. Expression of yhcQ and yhcP was necessary and sufficient for suppression of the pHBA hypersensitivity of the yhcS mutant. Only a few aromatic carboxylic acids of hundreds of diverse compounds tested were defined as substrates of the YhcQP efflux pump. Thus, we propose renaming yhcS , yhcR , yhcQ , and yhcP , to reflect their role in aromatic carboxylic acid efflux, to aaeR , aaeX , aaeA , and aaeB , respectively. The role of pHBA in normal E. coli metabolism and the highly regulated expression of the AaeAB efflux system suggests that the physiological role may be as a “metabolic relief valve” to alleviate toxic effects of imbalanced metabolism.

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Publisher
American Society For Microbiology
Copyright
Copyright © 2004 by the American society for Microbiology.
ISSN
0021-9193
eISSN
1098-5530
DOI
10.1128/JB.186.21.7196-7204.2004
pmid
15489430
Publisher site
See Article on Publisher Site

Abstract

Characterization of the Escherichia coli AaeAB Efflux Pump: a Metabolic Relief Valve? Tina K. Van Dyk * , Lori J. Templeton , Keith A. Cantera , Pamela L. Sharpe , and F. Sima Sariaslani DuPont Company, Central Research and Development Department, Wilmington, Delaware ABSTRACT Treatment of Escherichia coli with p -hydroxybenzoic acid (pHBA) resulted in upregulation of yhcP , encoding a protein of the putative efflux protein family. Also upregulated were the adjacent genes yhcQ , encoding a protein of the membrane fusion protein family, and yhcR , encoding a small protein without a known or suggested function. The function of the upstream, divergently transcribed gene yhcS , encoding a regulatory protein of the LysR family, in regulating expression of yhcRQP was shown. Furthermore, it was demonstrated that several aromatic carboxylic acid compounds serve as inducers of yhcRQP expression. The efflux function encoded by yhcP was proven by the hypersensitivity to pHBA of a yhcP mutant strain. A yhcS mutant strain was also hypersensitive to pHBA. Expression of yhcQ and yhcP was necessary and sufficient for suppression of the pHBA hypersensitivity of the yhcS mutant. Only a few aromatic carboxylic acids of hundreds of diverse compounds tested were defined as substrates of the YhcQP efflux pump. Thus, we propose renaming yhcS , yhcR , yhcQ , and yhcP , to reflect their role in aromatic carboxylic acid efflux, to aaeR , aaeX , aaeA , and aaeB , respectively. The role of pHBA in normal E. coli metabolism and the highly regulated expression of the AaeAB efflux system suggests that the physiological role may be as a “metabolic relief valve” to alleviate toxic effects of imbalanced metabolism.

Journal

Journal of BacteriologyAmerican Society For Microbiology

Published: Nov 1, 2004

There are no references for this article.