Abstract

Receive: RSS Feeds, eTOCs, free email alerts (when new articles cite this article), more» Information about commercial reprint orders: http://jvi.asm.org/site/misc/reprints.xhtml To subscribe to to another ASM Journal go to: http://journals.asm.org/site/subscriptions/ JOURNAL OF VIROLOGY, Feb., 1967, p. 238-240 Copyright © 1967 American Society for Microbiology Vol. 1, No. 1 Printed in U.S.A. SAUL KIT, DEL R. DUBBS, AND MILTON ANKEN Division of Biochemical Virology, Baylor University College of Medicine, Houston, Texas Received for publication 21 September 1966 Previous studies have shown that, after infection by herpes simplex virus (HSV) or vaccinia virus, thymidine kinase activity was induced in either LM mouse fibroblast cells or LM(TK-) cells, a mutant subline lacking this enzyme activity. At 5 to 7 hr after infection, the enzyme activities induced by these viruses in the LM(TK-) cells were about three times as high as those found in exponentially growing cultures of noninfected LM cells. Mutant vaccinia virus and HSV strains have been isolated which lack enzyme-inducing activity (D. R. Dubbs and S. Kit, Virology 22:214, 1964; Virology 22:493, 1964). Partially defective HSV mutants have been obtained which fail to induce the enzyme at 37 C, but which induce about one-tenth the enzyme level attained with