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Comparison of BIOMED-2 Versus Laboratory-Developed Polymerase Chain Reaction Assays for Detecting T-Cell Receptor-{gamma} Gene Rearrangements

Comparison of BIOMED-2 Versus Laboratory-Developed Polymerase Chain Reaction Assays for Detecting... Detecting clonal T-cell receptor ( TCR )- gene rearrangements (GRs) is an important adjunct test for diagnosing T-cell lymphoma. We compared a recently described assay (BIOMED-2 protocol), which targets multiple variable (V) gene segments in two polymerase chain reaction (PCR) reactions (multi-V), with a frequently referenced assay that targets a single V gene segment in four separate PCR reactions (mono-V). A total of 144 consecutive clinical DNA samples were prospectively tested for T-cell clonality by PCR using laboratory-developed mono-V and commercial multi-V primer sets for TCR - GR. The combination of TCR -β, mono-V TCR - and multi-V TCR - detected more clonal cases (68/144, 47%) than any individual PCR assay. We detected clonal TCR -β GR in 47/68 (69%) cases. Using either mono-V or multi-V TCR - primers, the sensitivities for detecting clonality were 52/68 (76%) or 51/68 (75%). Using both mono-V and multi-V TCR - primers improved the sensitivity for detecting clonality, 60/68 (88%). Combining either mono-V or multi-V TCR - primers with TCR -β primers also improved the sensitivity, 64/68 (94%). Significantly, TCR - V11 GRs could only be detected using the mono-V-PCR primers. We conclude that using more than one T-cell PCR assay can enhance the overall sensitivity for detecting T-cell clonality. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Molecular Diagnostics American Society for Investigative Pathology

Comparison of BIOMED-2 Versus Laboratory-Developed Polymerase Chain Reaction Assays for Detecting T-Cell Receptor-{gamma} Gene Rearrangements

Comparison of BIOMED-2 Versus Laboratory-Developed Polymerase Chain Reaction Assays for Detecting T-Cell Receptor-{gamma} Gene Rearrangements

Journal of Molecular Diagnostics , Volume 12 (2): 226 – Mar 1, 2010

Abstract

Detecting clonal T-cell receptor ( TCR )- gene rearrangements (GRs) is an important adjunct test for diagnosing T-cell lymphoma. We compared a recently described assay (BIOMED-2 protocol), which targets multiple variable (V) gene segments in two polymerase chain reaction (PCR) reactions (multi-V), with a frequently referenced assay that targets a single V gene segment in four separate PCR reactions (mono-V). A total of 144 consecutive clinical DNA samples were prospectively tested for T-cell clonality by PCR using laboratory-developed mono-V and commercial multi-V primer sets for TCR - GR. The combination of TCR -β, mono-V TCR - and multi-V TCR - detected more clonal cases (68/144, 47%) than any individual PCR assay. We detected clonal TCR -β GR in 47/68 (69%) cases. Using either mono-V or multi-V TCR - primers, the sensitivities for detecting clonality were 52/68 (76%) or 51/68 (75%). Using both mono-V and multi-V TCR - primers improved the sensitivity for detecting clonality, 60/68 (88%). Combining either mono-V or multi-V TCR - primers with TCR -β primers also improved the sensitivity, 64/68 (94%). Significantly, TCR - V11 GRs could only be detected using the mono-V-PCR primers. We conclude that using more than one T-cell PCR assay can enhance the overall sensitivity for detecting T-cell clonality.

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Publisher
American Society for Investigative Pathology
Copyright
Copyright © 2010 by the American Society for Investigative Pathology and the Association for Molecular Pathology.
ISSN
1525-1578
eISSN
1525-1578
DOI
10.2353/jmoldx.2010.090042
pmid
20181819
Publisher site
See Article on Publisher Site

Abstract

Detecting clonal T-cell receptor ( TCR )- gene rearrangements (GRs) is an important adjunct test for diagnosing T-cell lymphoma. We compared a recently described assay (BIOMED-2 protocol), which targets multiple variable (V) gene segments in two polymerase chain reaction (PCR) reactions (multi-V), with a frequently referenced assay that targets a single V gene segment in four separate PCR reactions (mono-V). A total of 144 consecutive clinical DNA samples were prospectively tested for T-cell clonality by PCR using laboratory-developed mono-V and commercial multi-V primer sets for TCR - GR. The combination of TCR -β, mono-V TCR - and multi-V TCR - detected more clonal cases (68/144, 47%) than any individual PCR assay. We detected clonal TCR -β GR in 47/68 (69%) cases. Using either mono-V or multi-V TCR - primers, the sensitivities for detecting clonality were 52/68 (76%) or 51/68 (75%). Using both mono-V and multi-V TCR - primers improved the sensitivity for detecting clonality, 60/68 (88%). Combining either mono-V or multi-V TCR - primers with TCR -β primers also improved the sensitivity, 64/68 (94%). Significantly, TCR - V11 GRs could only be detected using the mono-V-PCR primers. We conclude that using more than one T-cell PCR assay can enhance the overall sensitivity for detecting T-cell clonality.

Journal

Journal of Molecular DiagnosticsAmerican Society for Investigative Pathology

Published: Mar 1, 2010

References