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IS DRIED BLOOD AS RELIABLE AS FRESH SERUM IN MAKING THE WIDAL TEST?

IS DRIED BLOOD AS RELIABLE AS FRESH SERUM IN MAKING THE WIDAL TEST? TABLE I. SHOWING WHAT PER CENT. OF THE WEIGHT OF FRESH BLOOD IS REPRESENTED BY THE WEIGHT OF THE AIR-DRIED BLOOD. Specimen number. Weight of fresh blood. Weight of air-dried blood. .0046 .0063 .006 .0148 .0089 Per cent. of original weight left after drying. .018 .023 .024 .061 .0405 25.5% 27.4 25. 24. 22. Average 24.78%. Approximately one fourth of the weight of the fresh blood is left after air-drying for twenty-four hours. It is clear, therefore, that a solution of 1 mg. of dried blood in .1 cc. of distilled water makes a solution of 1:100 of the dried blood or 1:25 of the whole blood. *Jour. Infect. Dis. Supplement 1. p. 316. Westbrook recommended thahe reaction with dried blood be madewith a dilution of 1:25 of the whole blood. It seemed desirable to us, therefore, to test a large number of normal bloods in this dilution, to determine what per cent. of these gives an agglutination of typhoid bacilli. WeTABLE II. SHOWING RESULTS OF AGGLUTINATION TESTS WITH DRIED NORMAL BLOODS, AND FRESH NORMAL SERA. Dilution and Result. Dilution and Result. Blood. 1:925. Blood. 1:25. t t ti t I I I Tests with normal sera. Sera.I http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American Journal of Public Health American Public Health Association

IS DRIED BLOOD AS RELIABLE AS FRESH SERUM IN MAKING THE WIDAL TEST?

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Publisher
American Public Health Association
Copyright
Copyright © by the American Public Health Association
ISSN
0090-0036
eISSN
1541-0048
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Abstract

TABLE I. SHOWING WHAT PER CENT. OF THE WEIGHT OF FRESH BLOOD IS REPRESENTED BY THE WEIGHT OF THE AIR-DRIED BLOOD. Specimen number. Weight of fresh blood. Weight of air-dried blood. .0046 .0063 .006 .0148 .0089 Per cent. of original weight left after drying. .018 .023 .024 .061 .0405 25.5% 27.4 25. 24. 22. Average 24.78%. Approximately one fourth of the weight of the fresh blood is left after air-drying for twenty-four hours. It is clear, therefore, that a solution of 1 mg. of dried blood in .1 cc. of distilled water makes a solution of 1:100 of the dried blood or 1:25 of the whole blood. *Jour. Infect. Dis. Supplement 1. p. 316. Westbrook recommended thahe reaction with dried blood be madewith a dilution of 1:25 of the whole blood. It seemed desirable to us, therefore, to test a large number of normal bloods in this dilution, to determine what per cent. of these gives an agglutination of typhoid bacilli. WeTABLE II. SHOWING RESULTS OF AGGLUTINATION TESTS WITH DRIED NORMAL BLOODS, AND FRESH NORMAL SERA. Dilution and Result. Dilution and Result. Blood. 1:925. Blood. 1:25. t t ti t I I I Tests with normal sera. Sera.I

Journal

American Journal of Public Health – American Public Health Association

Published: Feb 1, 1914

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