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A COMPARISON BETWEEN THE SAND FILTRATION METHOD AND THE BUBBLING METHOD FOR ENUMERATING BACTERIA IN AIR

A COMPARISON BETWEEN THE SAND FILTRATION METHOD AND THE BUBBLING METHOD FOR ENUMERATING BACTERIA... Read before the Laboratory Section, American Public Health Association, Washington, D. C., September, 1912. Rettger has described in the Journal of Medical Research (N. S. v. 22, p. 461) a method for enumerating bacteria in air whereby a measured volume of air is drawn through 5 cc. of physiological salt solution, and portions of the liquid subsequently plated for counting. He made no comparisons with other methods, but showed that when the air was drawn through two tubes connected tandem, very few or no bacteria passed into the second tube. To compare the results obtained by this method with the results obtained by the sand filtration method, we have made a number of parallel tests. Each of us worked independently, devising his own apparatus and technique; and the results were finally compared. In consequence ,of this plan the details of making the trials differed slightly, but we were led to essentially the same conclusions. The apparatus, as devised by Rettger, consists of a glass tube with a small round bulb at one end. The bulb has eight or ten small perforations which allow the air to be passed at a rapid rate and yet divide it to such http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American Journal of Public Health American Public Health Association

A COMPARISON BETWEEN THE SAND FILTRATION METHOD AND THE BUBBLING METHOD FOR ENUMERATING BACTERIA IN AIR

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Publisher
American Public Health Association
Copyright
Copyright © by the American Public Health Association
ISSN
0090-0036
eISSN
1541-0048
Publisher site
See Article on Publisher Site

Abstract

Read before the Laboratory Section, American Public Health Association, Washington, D. C., September, 1912. Rettger has described in the Journal of Medical Research (N. S. v. 22, p. 461) a method for enumerating bacteria in air whereby a measured volume of air is drawn through 5 cc. of physiological salt solution, and portions of the liquid subsequently plated for counting. He made no comparisons with other methods, but showed that when the air was drawn through two tubes connected tandem, very few or no bacteria passed into the second tube. To compare the results obtained by this method with the results obtained by the sand filtration method, we have made a number of parallel tests. Each of us worked independently, devising his own apparatus and technique; and the results were finally compared. In consequence ,of this plan the details of making the trials differed slightly, but we were led to essentially the same conclusions. The apparatus, as devised by Rettger, consists of a glass tube with a small round bulb at one end. The bulb has eight or ten small perforations which allow the air to be passed at a rapid rate and yet divide it to such

Journal

American Journal of Public HealthAmerican Public Health Association

Published: Feb 1, 1913

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