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The Medial Preoptic Area and Its Projections to the Ventral Tegmental Area and the Periaqueductal Gray Are Activated in Response to Social Play Behavior in Juvenile Rats

The Medial Preoptic Area and Its Projections to the Ventral Tegmental Area and the Periaqueductal... The medial preoptic area (MPOA) is well known for its role in sexual and maternal behaviors. This region also plays an important role in affiliative social behaviors outside reproductive contexts. We recently demonstrated that the MPOA is a central nucleus in which opioids govern highly rewarding social play behavior in adolescent rats. However, the neural circuit mechanisms underlying MPOA-mediated social play remain largely unresolved. We hypothesized that the MPOA unites a complementary neural system through which social play induces reward via a projection to the ventral tegmental area (VTA) and reduces a negative affective state through a projection to the periaqueductal gray (PAG). To test whether the two projection pathways are activated in response to social play behavior, we combined retrograde tract tracing with immediate early gene (IEG) expression and immunofluorescent labeling to identify opioid-sensitive projection pathways from the MPOA to VTA and PAG that are activated after performance of social play. Retrograde tracer, fluoro-gold (FG), was microinjected into the VTA or PAG. IEG expression (i.e., Egr1) was assessed and triple immunofluorescent labeling for mu opioid receptor (MOR), Egr1, and FG in the MPOA was performed after social play. We revealed that play animals displayed an increase in neurons double labeled for Egr1 + FG and triple labeled for MOR + Egr1 + FG in the MPOA projecting to both the VTA and PAG when compared to no-play rats. The increased activation of projection neurons that express MORs from MPOA to VTA or PAG after social play suggests that opioids may act through these projection pathways to govern social play. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Behavioral Neuroscience American Psychological Association

The Medial Preoptic Area and Its Projections to the Ventral Tegmental Area and the Periaqueductal Gray Are Activated in Response to Social Play Behavior in Juvenile Rats

Zhao, Changjiu; Riters, Lauren V.
Behavioral Neuroscience , Volume 137 (4): 13 – Aug 6, 2023
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Publisher
American Psychological Association
Copyright
© 2023 American Psychological Association
ISSN
0735-7044
eISSN
1939-0084
DOI
10.1037/bne0000555
Publisher site
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Abstract

The medial preoptic area (MPOA) is well known for its role in sexual and maternal behaviors. This region also plays an important role in affiliative social behaviors outside reproductive contexts. We recently demonstrated that the MPOA is a central nucleus in which opioids govern highly rewarding social play behavior in adolescent rats. However, the neural circuit mechanisms underlying MPOA-mediated social play remain largely unresolved. We hypothesized that the MPOA unites a complementary neural system through which social play induces reward via a projection to the ventral tegmental area (VTA) and reduces a negative affective state through a projection to the periaqueductal gray (PAG). To test whether the two projection pathways are activated in response to social play behavior, we combined retrograde tract tracing with immediate early gene (IEG) expression and immunofluorescent labeling to identify opioid-sensitive projection pathways from the MPOA to VTA and PAG that are activated after performance of social play. Retrograde tracer, fluoro-gold (FG), was microinjected into the VTA or PAG. IEG expression (i.e., Egr1) was assessed and triple immunofluorescent labeling for mu opioid receptor (MOR), Egr1, and FG in the MPOA was performed after social play. We revealed that play animals displayed an increase in neurons double labeled for Egr1 + FG and triple labeled for MOR + Egr1 + FG in the MPOA projecting to both the VTA and PAG when compared to no-play rats. The increased activation of projection neurons that express MORs from MPOA to VTA or PAG after social play suggests that opioids may act through these projection pathways to govern social play.

Journal

Behavioral Neuroscience – American Psychological Association

Published: Aug 6, 2023

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