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Whole Mounts of the Corneal Endothelium

Whole Mounts of the Corneal Endothelium Abstract Introduction Meridional sectioning of the cornea allows only very limited examination of the endothelium; therefore, other methods have been devised which allow larger portions or even the entire endothelium to be studied in one preparation.1-3 However, these alternate methods can be very time consuming and require a considerable degree of dexterity. Further, some of these methods have not been applied successfully to human corneal endothelia.When applied to edematous corneae, those techniques which require separation of Descemet's membrane and of endothelium from the remaining cornea may cause some loss of endothelial cells from Descemet's membrane. This loss is obvious in a comparison of the endothelium of an edematous cornea before and after separation with Descemet's from the underlying stroma. Method The method described below provides a permanent preparation of complete endothelium with adequate visualization of cellular details, and has the further advantages of simplicity, decrease in possibility of cell References 1. Paragon water soluble mounting medium; Paragon C & C Co., Inc., New York 10058. 2. Irvine, A. R., and Irvine, A. R., Jr.: Variations in Normal Human Corneal Endothelium , Amer J Ophthal 36:1279-1285, 1953. 3. Nagano: Untersuchungen zur Pathologie des Hornhautendothels , Arch Augenheilk 76:26-68, 1914. 4. von Sallmann, L.; Caravaggio, M. S.; and Grimes, P.: Studies on Corneal Endothelium of Rabbit: I: Cell Division and Growth , Amer J Ophthal 51:956-966 (May, (pt 2) ), 1961. 5. Manual of Histologic and Special Staining Technics , Armed Forces Institute of Pathology, Washington, DC, 1957, p 32. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Whole Mounts of the Corneal Endothelium

Archives of Ophthalmology , Volume 73 (4) – Apr 1, 1965

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Publisher
American Medical Association
Copyright
Copyright © 1965 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1965.00970030557020
Publisher site
See Article on Publisher Site

Abstract

Abstract Introduction Meridional sectioning of the cornea allows only very limited examination of the endothelium; therefore, other methods have been devised which allow larger portions or even the entire endothelium to be studied in one preparation.1-3 However, these alternate methods can be very time consuming and require a considerable degree of dexterity. Further, some of these methods have not been applied successfully to human corneal endothelia.When applied to edematous corneae, those techniques which require separation of Descemet's membrane and of endothelium from the remaining cornea may cause some loss of endothelial cells from Descemet's membrane. This loss is obvious in a comparison of the endothelium of an edematous cornea before and after separation with Descemet's from the underlying stroma. Method The method described below provides a permanent preparation of complete endothelium with adequate visualization of cellular details, and has the further advantages of simplicity, decrease in possibility of cell References 1. Paragon water soluble mounting medium; Paragon C & C Co., Inc., New York 10058. 2. Irvine, A. R., and Irvine, A. R., Jr.: Variations in Normal Human Corneal Endothelium , Amer J Ophthal 36:1279-1285, 1953. 3. Nagano: Untersuchungen zur Pathologie des Hornhautendothels , Arch Augenheilk 76:26-68, 1914. 4. von Sallmann, L.; Caravaggio, M. S.; and Grimes, P.: Studies on Corneal Endothelium of Rabbit: I: Cell Division and Growth , Amer J Ophthal 51:956-966 (May, (pt 2) ), 1961. 5. Manual of Histologic and Special Staining Technics , Armed Forces Institute of Pathology, Washington, DC, 1957, p 32.

Journal

Archives of OphthalmologyAmerican Medical Association

Published: Apr 1, 1965

References