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Vernal Conjunctivitis: Model Studies in Guinea Pigs Immunized Topically With Fluoresceinyl Ovalbumin

Vernal Conjunctivitis: Model Studies in Guinea Pigs Immunized Topically With Fluoresceinyl Ovalbumin Abstract • Repeated topical applications of fluoresceinyl ovalbumin (FL-OA) to the conjunctival sac of guinea pigs sensitized them for conjunctival type 1 hypersensitivity reactions and mast cell degranulation. Guinea pigs infected with Ascaris suum, with high titers of circulating anti-A suum IgE and IgG1 antibody, also produced conjunctival type 1 reactions on topical challenge with A suum antigen. These reactions were no more intense than those of animals topically sensitized and challenged with FL-OA, which in some instances had no detectable serum homocytotropic antibody. Persistently reactive animals that had undergone repeated type 1 conjunctival reactions had histological findings (eg, papillary changes with extensive epithelial eosinophil infiltrates, epithelial thickening or thinning, numerous goblet cells, subepithelial lymphoid cell infiltrates, and new vessel formation) resembling those of human atopic vernal conjunctivitis. References 1. Neumann E, Gutmann MJ, Blumenkrantz N, et al: A review of 400 cases of vernal conjunctivitis . Am J Ophthalmol 1959;47:166-172. 2. Rice NSC, Easty D, Garner A, et al: Vernal kerato-conjunctivitis and its management . Trans Ophthalmol Soc UK 1971;91:483-489. 3. Rockey JH, Donnelly JJ, Stromberg BE, et al: Immunopathology of ascarid infection of the eye: The role of IgE antibodies and mast cells . Arch Ophthalmol 1981;99:1831-1840.Crossref 4. Allansmith MR, Hahn GS, Simon MA: Tissue, tear and serum IgE concentrations in vernal conjunctivitis . Am J Ophthalmol 1976;81:506-511. 5. Easty D, Rice NSC, Jones BR: Disodium cromoglycate (Intal) in the treatment of vernal keratoconjunctivitis . Trans Ophthalmol Soc UK 1971;91:491-499. 6. Lowry OH, Rosebrough NJ, Farr L, et al: Determination of proteins with the Folin reagent . J Biol Chem 1951;193:265-268. 7. Donnelly JJ, Rockey JH, Soulsby EJL: Intraocular IgE antibody induced in guinea pigs with Ascaris suum larvae . Invest Ophthalmol Vis Sci 1977;16:976-981. 8. Ishizaka T: Biochemical analysis of triggering signals induced by bridging of IgE receptors . Fed Proc 1982;41:17-21. 9. MacGlashan DW, Schleimer RP, Peters SP, et al: Comparative studies of human basophils and mast cells . Fed Proc 1983;42:2504-2509. 10. Schulman ES, Kagey-Sobotka A, MacGlashan DW, et al: Heterogeneity of mast cells . J Immunol 1983;131:1936-1941. 11. Marom Z, Casale TB: Mast cells and their mediators . Ann Allergy 1983;50:367-370. 12. Parish WE: Short term anaphylactic IgG antibodies in human sera . Lancet 1970;2:591-592.Crossref 13. Gwynn CM, Ingram J, Almousawi T, et al: Bronchial provocation tests in atopic patients with allergen-specific IgG4 antibodies . Lancet 1982;1:254-256.Crossref 14. Dobson C, Rockey JH, Soulsby EJL: Immunoglobulin E antibodies in guinea pigs: Characterization of monomeric and polymeric components . J Immunol 1971;107:1431-1439. 15. Rockey JH, John T, Donnelly JJ, et al: In vitro interaction of eosinophils from ascarid-infected eyes with Ascaris suum and Toxocara canis larvae . Invest Ophthalmol Vis Sci 1983; 24:1346-1357. 16. Kagey-Sobotka A, MacGlashan DW, Lichtenstein LM: Role of receptor aggregation in triggering IgE-mediated reactions . Fed Proc 1982;41:12-16. 17. Dvorak AM, Galli SJ, Morgan E, et al: Anaphylactic degranulation of guinea pig basophilic leukocytes: II. Evidence for regranulation of mature basophils during recovery from degranulation in vitro . Lab Invest 1982;46:461-475. 18. Ihle JN, Rebar L, Keller J, et al: Interleukin 3: Possible roles in the regulation of lymphocyte differentiation and growth . Immunol Rev 1982;63:5-32.Crossref 19. Rockey JH, Donnelly JJ, Stromberg BE, et al: Immunopathology of Toxocara canis and Ascaris suum infections of the eye: The role of the eosinophil . Invest Ophthalmol Vis Sci 1979; 18:1172-1184. 20. Schrader JW, Scollay R, Battye F: Intra-mucosal lymphocytes of the gut: Lyt-2 and Thy-1 phenotype of the granulated cells and evidence for the presence of both T cells and mast cell precursors . J Immunol 1983;130:558-564. 21. Kennedy DA, Sullivan TJ, Sylvester P, et al: Diacylglycerol metabolism in mast cells: A potential role in membrane fusion and arachidonic acid release . J Exp Med 1979;150:1039-1044.Crossref 22. Winslow CM, Austen KF: Enzymatic regulation of mast cell activation and secretion by adenylate cyclase and cyclic AMP-dependent kinases . Fed Proc 1982;41:22-29. 23. Austen KF, Wasserman SI, Goetzl EJ: Mast cell derived mediators: Structural and functional diversity and regulation of expression, in Johansson SGO , Strandberg K, Uvnas B (eds): Molecular and Biological Aspects of Acute Allergic Reaction . New York, Plenum Publishing Co, 1976, pp 293-320. 24. Montgomery PC, Ayyildiz A, Lemaitre-Coelho IM, et al: Induction and expression of antibodies in secretions: The ocular immune system . Ann NY Acad Sci 1983;409:428-440.Crossref 25. Abelson MB, Udell IJ, Weston JH: Conjunctival eosinophils in compound 48/80-rabbit model . Arch Ophthalmol 1983;101:631-633.Crossref 26. Friedlaender MH, Cyr R: Contact sensitivity in the guinea pig eye . Curr Eye Res 1981;1:403-407.Crossref 27. Abelson MB, Madiwale N, Weston JH: Conjunctival eosinophils in allergic ocular disease . Arch Ophthalmol 1983;101:555-556.Crossref 28. Udell IJ, Gleich GJ, Allansmith MR, et al: Eosinophil granule major basic protein and Charcot-Leyden crystal protein in human tears . Am J Ophthalmol 1981;92:824-828. 29. Morgan G: The pathology of vernal conjunctivitis . Trans Ophthalmol Soc UK 1971; 91:467-478. 30. Allansmith MR, Korb DR, Greiner JV, et al: Giant papillary conjunctivitis in contact lens wearers . Am J Ophthalmol 1977;83:697-708. 31. Spring TF: Reaction to hydrophilic lenses . Med J Aust 1974;1:449-450. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Vernal Conjunctivitis: Model Studies in Guinea Pigs Immunized Topically With Fluoresceinyl Ovalbumin

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Publisher
American Medical Association
Copyright
Copyright © 1984 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1984.01040031367029
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Abstract

Abstract • Repeated topical applications of fluoresceinyl ovalbumin (FL-OA) to the conjunctival sac of guinea pigs sensitized them for conjunctival type 1 hypersensitivity reactions and mast cell degranulation. Guinea pigs infected with Ascaris suum, with high titers of circulating anti-A suum IgE and IgG1 antibody, also produced conjunctival type 1 reactions on topical challenge with A suum antigen. These reactions were no more intense than those of animals topically sensitized and challenged with FL-OA, which in some instances had no detectable serum homocytotropic antibody. Persistently reactive animals that had undergone repeated type 1 conjunctival reactions had histological findings (eg, papillary changes with extensive epithelial eosinophil infiltrates, epithelial thickening or thinning, numerous goblet cells, subepithelial lymphoid cell infiltrates, and new vessel formation) resembling those of human atopic vernal conjunctivitis. References 1. Neumann E, Gutmann MJ, Blumenkrantz N, et al: A review of 400 cases of vernal conjunctivitis . Am J Ophthalmol 1959;47:166-172. 2. Rice NSC, Easty D, Garner A, et al: Vernal kerato-conjunctivitis and its management . Trans Ophthalmol Soc UK 1971;91:483-489. 3. Rockey JH, Donnelly JJ, Stromberg BE, et al: Immunopathology of ascarid infection of the eye: The role of IgE antibodies and mast cells . Arch Ophthalmol 1981;99:1831-1840.Crossref 4. Allansmith MR, Hahn GS, Simon MA: Tissue, tear and serum IgE concentrations in vernal conjunctivitis . Am J Ophthalmol 1976;81:506-511. 5. Easty D, Rice NSC, Jones BR: Disodium cromoglycate (Intal) in the treatment of vernal keratoconjunctivitis . Trans Ophthalmol Soc UK 1971;91:491-499. 6. Lowry OH, Rosebrough NJ, Farr L, et al: Determination of proteins with the Folin reagent . J Biol Chem 1951;193:265-268. 7. Donnelly JJ, Rockey JH, Soulsby EJL: Intraocular IgE antibody induced in guinea pigs with Ascaris suum larvae . Invest Ophthalmol Vis Sci 1977;16:976-981. 8. Ishizaka T: Biochemical analysis of triggering signals induced by bridging of IgE receptors . Fed Proc 1982;41:17-21. 9. MacGlashan DW, Schleimer RP, Peters SP, et al: Comparative studies of human basophils and mast cells . Fed Proc 1983;42:2504-2509. 10. Schulman ES, Kagey-Sobotka A, MacGlashan DW, et al: Heterogeneity of mast cells . J Immunol 1983;131:1936-1941. 11. Marom Z, Casale TB: Mast cells and their mediators . Ann Allergy 1983;50:367-370. 12. Parish WE: Short term anaphylactic IgG antibodies in human sera . Lancet 1970;2:591-592.Crossref 13. Gwynn CM, Ingram J, Almousawi T, et al: Bronchial provocation tests in atopic patients with allergen-specific IgG4 antibodies . Lancet 1982;1:254-256.Crossref 14. Dobson C, Rockey JH, Soulsby EJL: Immunoglobulin E antibodies in guinea pigs: Characterization of monomeric and polymeric components . J Immunol 1971;107:1431-1439. 15. Rockey JH, John T, Donnelly JJ, et al: In vitro interaction of eosinophils from ascarid-infected eyes with Ascaris suum and Toxocara canis larvae . Invest Ophthalmol Vis Sci 1983; 24:1346-1357. 16. Kagey-Sobotka A, MacGlashan DW, Lichtenstein LM: Role of receptor aggregation in triggering IgE-mediated reactions . Fed Proc 1982;41:12-16. 17. Dvorak AM, Galli SJ, Morgan E, et al: Anaphylactic degranulation of guinea pig basophilic leukocytes: II. Evidence for regranulation of mature basophils during recovery from degranulation in vitro . Lab Invest 1982;46:461-475. 18. Ihle JN, Rebar L, Keller J, et al: Interleukin 3: Possible roles in the regulation of lymphocyte differentiation and growth . Immunol Rev 1982;63:5-32.Crossref 19. Rockey JH, Donnelly JJ, Stromberg BE, et al: Immunopathology of Toxocara canis and Ascaris suum infections of the eye: The role of the eosinophil . Invest Ophthalmol Vis Sci 1979; 18:1172-1184. 20. Schrader JW, Scollay R, Battye F: Intra-mucosal lymphocytes of the gut: Lyt-2 and Thy-1 phenotype of the granulated cells and evidence for the presence of both T cells and mast cell precursors . J Immunol 1983;130:558-564. 21. Kennedy DA, Sullivan TJ, Sylvester P, et al: Diacylglycerol metabolism in mast cells: A potential role in membrane fusion and arachidonic acid release . J Exp Med 1979;150:1039-1044.Crossref 22. Winslow CM, Austen KF: Enzymatic regulation of mast cell activation and secretion by adenylate cyclase and cyclic AMP-dependent kinases . Fed Proc 1982;41:22-29. 23. Austen KF, Wasserman SI, Goetzl EJ: Mast cell derived mediators: Structural and functional diversity and regulation of expression, in Johansson SGO , Strandberg K, Uvnas B (eds): Molecular and Biological Aspects of Acute Allergic Reaction . New York, Plenum Publishing Co, 1976, pp 293-320. 24. Montgomery PC, Ayyildiz A, Lemaitre-Coelho IM, et al: Induction and expression of antibodies in secretions: The ocular immune system . Ann NY Acad Sci 1983;409:428-440.Crossref 25. Abelson MB, Udell IJ, Weston JH: Conjunctival eosinophils in compound 48/80-rabbit model . Arch Ophthalmol 1983;101:631-633.Crossref 26. Friedlaender MH, Cyr R: Contact sensitivity in the guinea pig eye . Curr Eye Res 1981;1:403-407.Crossref 27. Abelson MB, Madiwale N, Weston JH: Conjunctival eosinophils in allergic ocular disease . Arch Ophthalmol 1983;101:555-556.Crossref 28. Udell IJ, Gleich GJ, Allansmith MR, et al: Eosinophil granule major basic protein and Charcot-Leyden crystal protein in human tears . Am J Ophthalmol 1981;92:824-828. 29. Morgan G: The pathology of vernal conjunctivitis . Trans Ophthalmol Soc UK 1971; 91:467-478. 30. Allansmith MR, Korb DR, Greiner JV, et al: Giant papillary conjunctivitis in contact lens wearers . Am J Ophthalmol 1977;83:697-708. 31. Spring TF: Reaction to hydrophilic lenses . Med J Aust 1974;1:449-450.

Journal

Archives of OphthalmologyAmerican Medical Association

Published: Nov 1, 1984

References