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Ultrastructural Changes in the Canine Ileal Mucosal Cell After Mesenteric Arterial Occlusion: A Sequential Study

Ultrastructural Changes in the Canine Ileal Mucosal Cell After Mesenteric Arterial Occlusion: A... Abstract Laparotomy was performed on seven healthy male mongrel dogs. The control sample of ileal mucosa was taken and a clamp applied to the superior mesenteric artery. Sections of ileal mucosa were obtained after clamping for electron microscopic study. By ten minutes after clamping, there was an accumulation of fluid infranuclearly with bulging of plasma and basement membranes. The mitochondria were somewhat swollen, however, lysosomes, rough endoplasmic reticulum, and nuclei were normal. Thirty minutes after clamping, the terminal web was clear with loss of protein normally present. Mitochondria were swollen with destruction of cristae, the rough endoplasmic reticula were dilated, and the outer membrane of the nucleus was separated. The lysosomes still appeared normal. Most striking was the large size of the intercellular spaces infranuclearly with fracturing of the intercellular membrane and lifting of the cell. References 1. Billroth T: Ueber Duodenalgeschwüre bei Septicämie . Med Wschr 45:705-709, 1867. 2. Ravdin IS: Primary ulcer of the jejunum . Ann Surg 85:873-878, 1927.Crossref 3. Lillehei RC: The intestinal factor in irreversible hemorrhagic shock . Surgery 42:1043, 1957. 4. Lillehei RC, MacLean LD: The intestinal factor in irreversible endotoxin shock . Ann Surg 148:513, 1958.Crossref 5. Bounous G, Brown RA, Mulder DS, et al: Abolition of "tryptic enteritis" in the shocked dog: Creation of an experimental model for study of human shock and its sequelae . Arch Surg 91:371-375, 1965.Crossref 6. Bounous G, et al: Phosphate metabolism in the intestine during hemorrhagic shock . J Trauma 4:424, 1964. 7. Chiu CJ, McArdle AH, Brown RA, et al: The intestinal mucosal lesion in low flow states: I. A morphological, hemodynamic, and metabolic reappraisal. Arch Surg, to be published. 8. Schumer WS, Kapica SK, Teng TL: Validity of the lysosomal theory in oligemic shock . Arch Surg 99:325-329, 1969.Crossref 9. Bounous G, McArdle AH: Release of intestinal enzymes in acute mesenteric ischemia . J Surg Res 9:339, 1969.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Surgery American Medical Association

Ultrastructural Changes in the Canine Ileal Mucosal Cell After Mesenteric Arterial Occlusion: A Sequential Study

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Publisher
American Medical Association
Copyright
Copyright © 1970 American Medical Association. All Rights Reserved.
ISSN
0004-0010
eISSN
1538-3644
DOI
10.1001/archsurg.1970.01340260194029
Publisher site
See Article on Publisher Site

Abstract

Abstract Laparotomy was performed on seven healthy male mongrel dogs. The control sample of ileal mucosa was taken and a clamp applied to the superior mesenteric artery. Sections of ileal mucosa were obtained after clamping for electron microscopic study. By ten minutes after clamping, there was an accumulation of fluid infranuclearly with bulging of plasma and basement membranes. The mitochondria were somewhat swollen, however, lysosomes, rough endoplasmic reticulum, and nuclei were normal. Thirty minutes after clamping, the terminal web was clear with loss of protein normally present. Mitochondria were swollen with destruction of cristae, the rough endoplasmic reticula were dilated, and the outer membrane of the nucleus was separated. The lysosomes still appeared normal. Most striking was the large size of the intercellular spaces infranuclearly with fracturing of the intercellular membrane and lifting of the cell. References 1. Billroth T: Ueber Duodenalgeschwüre bei Septicämie . Med Wschr 45:705-709, 1867. 2. Ravdin IS: Primary ulcer of the jejunum . Ann Surg 85:873-878, 1927.Crossref 3. Lillehei RC: The intestinal factor in irreversible hemorrhagic shock . Surgery 42:1043, 1957. 4. Lillehei RC, MacLean LD: The intestinal factor in irreversible endotoxin shock . Ann Surg 148:513, 1958.Crossref 5. Bounous G, Brown RA, Mulder DS, et al: Abolition of "tryptic enteritis" in the shocked dog: Creation of an experimental model for study of human shock and its sequelae . Arch Surg 91:371-375, 1965.Crossref 6. Bounous G, et al: Phosphate metabolism in the intestine during hemorrhagic shock . J Trauma 4:424, 1964. 7. Chiu CJ, McArdle AH, Brown RA, et al: The intestinal mucosal lesion in low flow states: I. A morphological, hemodynamic, and metabolic reappraisal. Arch Surg, to be published. 8. Schumer WS, Kapica SK, Teng TL: Validity of the lysosomal theory in oligemic shock . Arch Surg 99:325-329, 1969.Crossref 9. Bounous G, McArdle AH: Release of intestinal enzymes in acute mesenteric ischemia . J Surg Res 9:339, 1969.Crossref

Journal

Archives of SurgeryAmerican Medical Association

Published: Aug 1, 1970

References