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Twenty Percent of Biopsy Specimens From Sun-Exposed Skin of Normal Young Adults Demonstrate Positive Immunofluorescence

Twenty Percent of Biopsy Specimens From Sun-Exposed Skin of Normal Young Adults Demonstrate... Abstract • Fifty normal healthy adults, aged 18 to 41 years, without a history of systemic diseases, dermatoses, or photosensitivity and who were not receiving medication were studied. Paired 3-mm punch biopsy specimens were obtained from the sun-exposed and the non-sun-exposed skin. The data from the study revealed a bright continuous band of immunofluorescence (IF) along the dermoepidermal junction in 10 (20%) of 50 sun-exposed skin biopsy specimens, as compared with none from non-sun-exposed skin biopsy specimens with the use of polyvalent antisera. Fractionated monospecific immunoglobulin demonstrated a bright continuous band of IF composed of IgG alone in one patient, IgA alone in two patients, IgG and IgA in combination in two patients, and the combination of IgG, IgM, and IgA in five patients. There was a statistically significant increase in positive IF in men (seven of 15) vs women (three of 35). This information suggests that in the examination of a patient suspected of having lesions of cutaneous lupus erythematosus, positive IF from sun-exposed skin is nonspecific and adds little information to the clinical and histopathologic findings. (Arch Dermatol. 1991;127:1006-1011) References 1. Burnham TK, Neblett TR, Fine G. The application of the fluorescent antibody technic to the investigation of lupus erythematosus and various dermatoses . J Invest Dermatol. 1963;41:451-456.Crossref 2. Burnham TK, Fine G. The immunofluorescent `band' test for lupus erythematosus, I: morphologic variations of the band of localized immunoglobulins at the dermal-epidermal junction in lupus erythematosus . Arch Dermatol. 1969;99:413-420.Crossref 3. Cormane RH. `Bound' globulin in skin of patients with chronic discoid lupus erythematosus and systemic lupus erythematosus . Lancet. 1964;1:534-535.Crossref 4. Burnham TK, Fine G, Neblett TR. Immunofluorescent `band' test for lupus erythematosus, II: employing skin lesions . Arch Dermatol. 1970;102:42-50.Crossref 5. Kalsbeek GL, Cormane RH. `Bound' complement in the skin of patients with chronic discoid lupus erythematosus and systemic lupus erythematosus . Lancet. 1964;2:178-180.Crossref 6. Tan EM, Kunkel HG. An immunofluorescent study of skin lesions in systemic lupus erythematosus . Arthritis Rheum. 1966;9:37-46.Crossref 7. Izuno GT. Cutaneous immunofluorescence . Clin Lab Med. 1986;6:85-102. 8. Harrist TJ, Mihm MC. The specificity and clinical usefulness of the lupus band test . Arthritis Rheum. 1980;23:479-490.Crossref 9. Sontheimer RD, Thomas JR, Gilliam JN. Subacute cutaneous lupus erythematosus: a cutaneous marker for a distinct lupus erythe matosus subset . Arch Dermatol. 1979;115:1409-1415.Crossref 10. Cormane RH, Ballieux RE, Kalsbeek GL. Classification of immunoglobulins in the dermo-epidermal junction in lupus erythematosus . Clin Exp Immunol. 1966;1:207-212. 11. Kalsbeek GL, Cormane RH. The occurrence of immunoglobulins in the dermo-epidermal junction of the skin in lupus erythematosus and related syndromes . Dermatologica. 1967;135:205-212.Crossref 12. Chorzelski T, Jablonska S, Blaszcyk M. Immunopathologic investigation in lupus erythematosus . J Invest Dermatol. 1969;52:333-338. 13. Pohle EL, Tuffanelli DL. Study of cutaneous lupus by immunohistochemical methods . Arch Dermatol. 1968;97:520-526.Crossref 14. Percy JS, Smyth CJ. The immunofluorescent skin test in systemic lupus erythematosus . JAMA. 1968;208:485-488.Crossref 15. Baart de la Faille-Kuyper EH, Cormane RH. The occurrence of certain serum factors in the dermal-epidermal junction and vessel wall of the skin in lupus erythematosus and other (skin) diseases . Acta Derm Venereol (Stockh). 1968;48:578-588. 16. Burnham TK, Fine G. The immunofluorescent `band' test for lupus erythematosus, III: employing clinically normal skin . Arch Dermatol. 1971;103:24-32.Crossref 17. Gilliam JN, Cheatum DE, Hurd ER, Stastny P, Ziff M. Immunoglobulin in clinically uninvolved skin in systemic lupus erythematosus . J Clin Invest. 1974;53:1434-1440.Crossref 18. Provost TT, Andres G, Maddison PJ, Reichlin M. Lupus band test in untreated SLE patients: correlation of immunoglobulin deposition in the skin of the extensor forearm with clinical renal disease and serological abnormalities . J Invest Dermatol. 1980;74:407-412.Crossref 19. Davis BM, Gilliam JN. Prognostic significance of subepidermal immune deposits in uninvolved skin of patients with systemic lupus erythematosus: a 10-year longitudinal study . J Invest Dermatol. 1984;83:242-247.Crossref 20. Grossman J, Callerame ML, Condemi JJ. Skin immunofluorescence studies on lupus erythematosus and other anti-nuclear-antibody positive diseases . Ann Intern Med. 1974;80:469-500.Crossref 21. Wertheimer D, Barland P. Clinical significance of immune deposits in the skin in SLE . Arthritis Rheum. 1976;19:1249-1255.Crossref 22. Dahl MV. Usefulness of direct immunofluorescence in patients with lupus erythematosus (forum discussion) . Arch Dermatol. 1983;119:1010-1017.Crossref 23. Ahmed AR, Provost TT. Incidence of a positive lupus band test using sun-exposed and unexposed skin . Arch Dermatol. 1979;115:228-229.Crossref 24. Jablonska S, Chorzelski T, Maciejowska E. The scope and limitations of immunofluorescence method in the diagnosis of lupus erythematosus . Br J Dermatol. 1970;83:242-247.Crossref 25. Baart de la Faille H, Baart de la Faille-Kuyper EH. Immunofluorescent studies of skin in rosacea . Dermatologica. 1969;139:49-54.Crossref 26. Salo OP. SLE-like deposition of immunoglobulins in the skin in rosacea . Ann Clin Res. L-1970;2: No. (28) . 27. Baart de la Faille-Kuyper EH, Van der Meer JB, Baart de la Faille H. An immunohistochemical study of the skin of healthy individuals . Acta Derm Venereol (Stockh). 1974;54:271-274. 28. Gryschwitz M, Keller J, Horstein OP. Deposits of immunoglobulins at the dermo-epidermal junction in chronic light-exposed skin: what is the value of the lupus band test? Clin Exp Dermatol. 1988;13:303-308.Crossref 29. Michel B, Milner Y, David K. Preservation of tissue-fixed immunoglobulins in skin biopsies of patients with lupus erythematosus and bullous disease . J Invest Dermatol. 1973;59:449-452.Crossref 30. Tuffanelli DL. Cutaneous immunopathology: recent observations . J Invest Dermatol. 1975;65:143-153.Crossref 31. Tuffanelli DL. Collagen vascular diseases . In: Stone J, ed. Immunology and Allergy. St Louis, Mo: CV Mosby Co; 1985:393-409. 32. Nesbitt LT. Cutaneous immunofluorescence in dermatomyositis . Int J Dermatol. 1980;19:270-278.Crossref 33. Natali PG, Tan EM. Experimental skin lesions in mice resembling systemic lupus erythematosus . Arthritis Rheum. 1973;16:579-589.Crossref 34. Sontheimer RD, Gilliam JN. Regional variation in the deposition of subepidermal immunoglobulin in NZB/W Fl mice: association with epidermal DNA synthesis . J Invest Dermatol. 1979;79:25-28.Crossref 35. Fogleman J, Gilliam JN. Local effect of trauma and ultraviolet light on subepidermal accumulation of immunoglobulin in NZB/W F1 mice . Clin Res. 1975;23:32.Abstract. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Dermatology American Medical Association

Twenty Percent of Biopsy Specimens From Sun-Exposed Skin of Normal Young Adults Demonstrate Positive Immunofluorescence

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Publisher
American Medical Association
Copyright
Copyright © 1991 American Medical Association. All Rights Reserved.
ISSN
0003-987X
eISSN
1538-3652
DOI
10.1001/archderm.1991.01680060080008
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Abstract

Abstract • Fifty normal healthy adults, aged 18 to 41 years, without a history of systemic diseases, dermatoses, or photosensitivity and who were not receiving medication were studied. Paired 3-mm punch biopsy specimens were obtained from the sun-exposed and the non-sun-exposed skin. The data from the study revealed a bright continuous band of immunofluorescence (IF) along the dermoepidermal junction in 10 (20%) of 50 sun-exposed skin biopsy specimens, as compared with none from non-sun-exposed skin biopsy specimens with the use of polyvalent antisera. Fractionated monospecific immunoglobulin demonstrated a bright continuous band of IF composed of IgG alone in one patient, IgA alone in two patients, IgG and IgA in combination in two patients, and the combination of IgG, IgM, and IgA in five patients. There was a statistically significant increase in positive IF in men (seven of 15) vs women (three of 35). This information suggests that in the examination of a patient suspected of having lesions of cutaneous lupus erythematosus, positive IF from sun-exposed skin is nonspecific and adds little information to the clinical and histopathologic findings. (Arch Dermatol. 1991;127:1006-1011) References 1. Burnham TK, Neblett TR, Fine G. The application of the fluorescent antibody technic to the investigation of lupus erythematosus and various dermatoses . J Invest Dermatol. 1963;41:451-456.Crossref 2. Burnham TK, Fine G. The immunofluorescent `band' test for lupus erythematosus, I: morphologic variations of the band of localized immunoglobulins at the dermal-epidermal junction in lupus erythematosus . Arch Dermatol. 1969;99:413-420.Crossref 3. Cormane RH. `Bound' globulin in skin of patients with chronic discoid lupus erythematosus and systemic lupus erythematosus . Lancet. 1964;1:534-535.Crossref 4. Burnham TK, Fine G, Neblett TR. Immunofluorescent `band' test for lupus erythematosus, II: employing skin lesions . Arch Dermatol. 1970;102:42-50.Crossref 5. Kalsbeek GL, Cormane RH. `Bound' complement in the skin of patients with chronic discoid lupus erythematosus and systemic lupus erythematosus . Lancet. 1964;2:178-180.Crossref 6. Tan EM, Kunkel HG. An immunofluorescent study of skin lesions in systemic lupus erythematosus . Arthritis Rheum. 1966;9:37-46.Crossref 7. Izuno GT. Cutaneous immunofluorescence . Clin Lab Med. 1986;6:85-102. 8. Harrist TJ, Mihm MC. The specificity and clinical usefulness of the lupus band test . Arthritis Rheum. 1980;23:479-490.Crossref 9. Sontheimer RD, Thomas JR, Gilliam JN. Subacute cutaneous lupus erythematosus: a cutaneous marker for a distinct lupus erythe matosus subset . Arch Dermatol. 1979;115:1409-1415.Crossref 10. Cormane RH, Ballieux RE, Kalsbeek GL. Classification of immunoglobulins in the dermo-epidermal junction in lupus erythematosus . Clin Exp Immunol. 1966;1:207-212. 11. Kalsbeek GL, Cormane RH. The occurrence of immunoglobulins in the dermo-epidermal junction of the skin in lupus erythematosus and related syndromes . Dermatologica. 1967;135:205-212.Crossref 12. Chorzelski T, Jablonska S, Blaszcyk M. Immunopathologic investigation in lupus erythematosus . J Invest Dermatol. 1969;52:333-338. 13. Pohle EL, Tuffanelli DL. Study of cutaneous lupus by immunohistochemical methods . Arch Dermatol. 1968;97:520-526.Crossref 14. Percy JS, Smyth CJ. The immunofluorescent skin test in systemic lupus erythematosus . JAMA. 1968;208:485-488.Crossref 15. Baart de la Faille-Kuyper EH, Cormane RH. The occurrence of certain serum factors in the dermal-epidermal junction and vessel wall of the skin in lupus erythematosus and other (skin) diseases . Acta Derm Venereol (Stockh). 1968;48:578-588. 16. Burnham TK, Fine G. The immunofluorescent `band' test for lupus erythematosus, III: employing clinically normal skin . Arch Dermatol. 1971;103:24-32.Crossref 17. Gilliam JN, Cheatum DE, Hurd ER, Stastny P, Ziff M. Immunoglobulin in clinically uninvolved skin in systemic lupus erythematosus . J Clin Invest. 1974;53:1434-1440.Crossref 18. Provost TT, Andres G, Maddison PJ, Reichlin M. Lupus band test in untreated SLE patients: correlation of immunoglobulin deposition in the skin of the extensor forearm with clinical renal disease and serological abnormalities . J Invest Dermatol. 1980;74:407-412.Crossref 19. Davis BM, Gilliam JN. Prognostic significance of subepidermal immune deposits in uninvolved skin of patients with systemic lupus erythematosus: a 10-year longitudinal study . J Invest Dermatol. 1984;83:242-247.Crossref 20. Grossman J, Callerame ML, Condemi JJ. Skin immunofluorescence studies on lupus erythematosus and other anti-nuclear-antibody positive diseases . Ann Intern Med. 1974;80:469-500.Crossref 21. Wertheimer D, Barland P. Clinical significance of immune deposits in the skin in SLE . Arthritis Rheum. 1976;19:1249-1255.Crossref 22. Dahl MV. Usefulness of direct immunofluorescence in patients with lupus erythematosus (forum discussion) . Arch Dermatol. 1983;119:1010-1017.Crossref 23. Ahmed AR, Provost TT. Incidence of a positive lupus band test using sun-exposed and unexposed skin . Arch Dermatol. 1979;115:228-229.Crossref 24. Jablonska S, Chorzelski T, Maciejowska E. The scope and limitations of immunofluorescence method in the diagnosis of lupus erythematosus . Br J Dermatol. 1970;83:242-247.Crossref 25. Baart de la Faille H, Baart de la Faille-Kuyper EH. Immunofluorescent studies of skin in rosacea . Dermatologica. 1969;139:49-54.Crossref 26. Salo OP. SLE-like deposition of immunoglobulins in the skin in rosacea . Ann Clin Res. L-1970;2: No. (28) . 27. Baart de la Faille-Kuyper EH, Van der Meer JB, Baart de la Faille H. An immunohistochemical study of the skin of healthy individuals . Acta Derm Venereol (Stockh). 1974;54:271-274. 28. Gryschwitz M, Keller J, Horstein OP. Deposits of immunoglobulins at the dermo-epidermal junction in chronic light-exposed skin: what is the value of the lupus band test? Clin Exp Dermatol. 1988;13:303-308.Crossref 29. Michel B, Milner Y, David K. Preservation of tissue-fixed immunoglobulins in skin biopsies of patients with lupus erythematosus and bullous disease . J Invest Dermatol. 1973;59:449-452.Crossref 30. Tuffanelli DL. Cutaneous immunopathology: recent observations . J Invest Dermatol. 1975;65:143-153.Crossref 31. Tuffanelli DL. Collagen vascular diseases . In: Stone J, ed. Immunology and Allergy. St Louis, Mo: CV Mosby Co; 1985:393-409. 32. Nesbitt LT. Cutaneous immunofluorescence in dermatomyositis . Int J Dermatol. 1980;19:270-278.Crossref 33. Natali PG, Tan EM. Experimental skin lesions in mice resembling systemic lupus erythematosus . Arthritis Rheum. 1973;16:579-589.Crossref 34. Sontheimer RD, Gilliam JN. Regional variation in the deposition of subepidermal immunoglobulin in NZB/W Fl mice: association with epidermal DNA synthesis . J Invest Dermatol. 1979;79:25-28.Crossref 35. Fogleman J, Gilliam JN. Local effect of trauma and ultraviolet light on subepidermal accumulation of immunoglobulin in NZB/W F1 mice . Clin Res. 1975;23:32.Abstract.

Journal

Archives of DermatologyAmerican Medical Association

Published: Jul 1, 1991

References