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The Use of Polymerase Chain Reaction Techniques to Detect Varicella-zoster Virus in Corneal Transplant Tissue

The Use of Polymerase Chain Reaction Techniques to Detect Varicella-zoster Virus in Corneal... Abstract In this issue of the Archives, Mietz and colleagues1 report the findings in the clinical records and correlate these with the histopathological findings in the corneal buttons following corneal transplant surgery in 14 patients with herpes zoster ophthalmicus. In addition to an evaluation using light microscopy, they utilized the polymerase chain reaction (PCR) technique combined with gel electrophoresis and hybridization for the detection of varicella-zoster virus (VZV) DNA. Similar PCR techniques have been used clinically to detect herpes simplex virus (HSV) in the corneal stroma and to detect VZV in skin lesions, the cerebrospinal fluid, and the corneal epithelium; these techniques have also been applied for research use to detect VZV and HSV DNA in the trigeminal and thoracic ganglia. Seven of the 14 corneal buttons were positive for VZV DNA with the finding correlated most strongly with the clinical findings of uveitis and the histopathological features of chronic References 1. Mietz H, Eis-Hübinger AM, Sundmacher R, Font RL. Detection of varicellazoster virus DNA in keratectomy specimens by use of the polymerase chain reaction . Arch Ophthalmol . 1997;115:590.Crossref 2. Yamamoto S, Tada R, Shimomura Y, Pavan-Langston D, Dunkel EC, Tano Y. Detecting varicella-zoster virus DNA in iridocyclitis using polymerase chain reaction: a case of zoster sine herpete . Arch Ophthalmol . 1995;113:1358-1359.Crossref 3. Neoh C, Harding SP, Saunders D, et al. Comparison of topical and oral acyclovir in early herpes zoster ophthalmicus . Eye . 1994;8:688-691.Crossref 4. Mullis KB, Faloona FA. Specific synthesis of DNA in vitro via a polymerasecatalyzed chain reaction . Methods Enzymol . 1987;155:335-350. 5. Nahass GT, Goldstein BA, Zhu WY, Serfling U, Penneys NS, Leonardi CL. Comparison of Tzanck smear, viral culture, and DNA diagnostic methods in detection of herpes simplex and varicella-zoster infection . JAMA . 1992;268:2541-2544.Crossref 6. Pavan-Langston D, Yamamoto S, Dunkel EC. Delayed herpes zoster pseudodendrites: polymerase chain reaction detection of viral DNA and a role for antiviral therapy . Arch Ophthalmol . 1995;113:1381-1385.Crossref 7. Stavrou P, Mitchell SM, Fox JD, Hope-Ross MW, Murray PI. Detection of varicellazoster virus DNA in ocular samples from patients with uveitis but no cutaneous eruption . Eye . 1994;8:684-687.Crossref 8. Alvarado JA, Underwood JL, Green WR, et al. Detection of herpes simplex viral DNA in the iridocorneal endothelial syndrome . Arch Ophthalmol . 1994;112:1601-1609.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

The Use of Polymerase Chain Reaction Techniques to Detect Varicella-zoster Virus in Corneal Transplant Tissue

Archives of Ophthalmology , Volume 115 (5) – May 1, 1997

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Publisher
American Medical Association
Copyright
Copyright © 1997 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1997.01100150666019
Publisher site
See Article on Publisher Site

Abstract

Abstract In this issue of the Archives, Mietz and colleagues1 report the findings in the clinical records and correlate these with the histopathological findings in the corneal buttons following corneal transplant surgery in 14 patients with herpes zoster ophthalmicus. In addition to an evaluation using light microscopy, they utilized the polymerase chain reaction (PCR) technique combined with gel electrophoresis and hybridization for the detection of varicella-zoster virus (VZV) DNA. Similar PCR techniques have been used clinically to detect herpes simplex virus (HSV) in the corneal stroma and to detect VZV in skin lesions, the cerebrospinal fluid, and the corneal epithelium; these techniques have also been applied for research use to detect VZV and HSV DNA in the trigeminal and thoracic ganglia. Seven of the 14 corneal buttons were positive for VZV DNA with the finding correlated most strongly with the clinical findings of uveitis and the histopathological features of chronic References 1. Mietz H, Eis-Hübinger AM, Sundmacher R, Font RL. Detection of varicellazoster virus DNA in keratectomy specimens by use of the polymerase chain reaction . Arch Ophthalmol . 1997;115:590.Crossref 2. Yamamoto S, Tada R, Shimomura Y, Pavan-Langston D, Dunkel EC, Tano Y. Detecting varicella-zoster virus DNA in iridocyclitis using polymerase chain reaction: a case of zoster sine herpete . Arch Ophthalmol . 1995;113:1358-1359.Crossref 3. Neoh C, Harding SP, Saunders D, et al. Comparison of topical and oral acyclovir in early herpes zoster ophthalmicus . Eye . 1994;8:688-691.Crossref 4. Mullis KB, Faloona FA. Specific synthesis of DNA in vitro via a polymerasecatalyzed chain reaction . Methods Enzymol . 1987;155:335-350. 5. Nahass GT, Goldstein BA, Zhu WY, Serfling U, Penneys NS, Leonardi CL. Comparison of Tzanck smear, viral culture, and DNA diagnostic methods in detection of herpes simplex and varicella-zoster infection . JAMA . 1992;268:2541-2544.Crossref 6. Pavan-Langston D, Yamamoto S, Dunkel EC. Delayed herpes zoster pseudodendrites: polymerase chain reaction detection of viral DNA and a role for antiviral therapy . Arch Ophthalmol . 1995;113:1381-1385.Crossref 7. Stavrou P, Mitchell SM, Fox JD, Hope-Ross MW, Murray PI. Detection of varicellazoster virus DNA in ocular samples from patients with uveitis but no cutaneous eruption . Eye . 1994;8:684-687.Crossref 8. Alvarado JA, Underwood JL, Green WR, et al. Detection of herpes simplex viral DNA in the iridocorneal endothelial syndrome . Arch Ophthalmol . 1994;112:1601-1609.Crossref

Journal

Archives of OphthalmologyAmerican Medical Association

Published: May 1, 1997

References