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THE TRICHOPHYTIN TEST: REPORT OF THREE HUNDRED AND FIFTY CASES

THE TRICHOPHYTIN TEST: REPORT OF THREE HUNDRED AND FIFTY CASES Abstract TECHNIC Although thirty years have elapsed since Plato and Neisser first prepared trichophytin and studied its diagnostic application in fungous diseases of the skin, the question of the specificity and the diagnostic value of the trichophytin reaction is still under discussion. Several trichophytins have been made by various authors from different fungi, but a standardized and universally recognized preparation is still lacking. Under such circumstances we felt justified in making a preparation of our own of recently isolated strains of fungi of common occurrence in our clinic. In our extract Trichophyton violaceum, Microsporon audouini and Epidermophyton interdigitale were used. These strains were grown separately in liquid medium— Sabouraud's broth (dextrose massée, 4 per cent; peptone, 1 per cent in water). After solution of all the materials, the medium was filtered through paper, distributed in several 250 cc. flasks, each of which contained about 150 cc. of medium, and sterilized http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Dermatology and Syphilology American Medical Association

THE TRICHOPHYTIN TEST: REPORT OF THREE HUNDRED AND FIFTY CASES

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Publisher
American Medical Association
Copyright
Copyright © 1933 American Medical Association. All Rights Reserved.
ISSN
0096-6029
DOI
10.1001/archderm.1933.01450040748002
Publisher site
See Article on Publisher Site

Abstract

Abstract TECHNIC Although thirty years have elapsed since Plato and Neisser first prepared trichophytin and studied its diagnostic application in fungous diseases of the skin, the question of the specificity and the diagnostic value of the trichophytin reaction is still under discussion. Several trichophytins have been made by various authors from different fungi, but a standardized and universally recognized preparation is still lacking. Under such circumstances we felt justified in making a preparation of our own of recently isolated strains of fungi of common occurrence in our clinic. In our extract Trichophyton violaceum, Microsporon audouini and Epidermophyton interdigitale were used. These strains were grown separately in liquid medium— Sabouraud's broth (dextrose massée, 4 per cent; peptone, 1 per cent in water). After solution of all the materials, the medium was filtered through paper, distributed in several 250 cc. flasks, each of which contained about 150 cc. of medium, and sterilized

Journal

Archives of Dermatology and SyphilologyAmerican Medical Association

Published: May 1, 1933

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