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THE SCARLET FEVER TOXIN AND THE DICK TEST

THE SCARLET FEVER TOXIN AND THE DICK TEST The work of the Dicks and of Zingher on scarlet fever, reported from time to time in The Journal, is of great theoretical and practical importance. We have tried to repeat some of it. The toxicogenic properties of nine strains of hemolytic streptococcus, obtained from the throats of scarlet fever patients in the first days of illness, were examined. Seven of them were found to be producers of toxin. In preparing the toxin, we have used the broth of fermented meat with 2 per cent. Witte's peptone with an alkalinity of pH 7.8. Five per cent. citrated or defibrinated sheep's blood was added to the broth. After incubation for four days at 37 C., the toxin was filtered through filter paper, and 0.25 per cent. phenol (carbolic acid) was added to it. The strength of the first portions of our toxin was 1:250, and later 1:500. At our disposal http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png JAMA American Medical Association

THE SCARLET FEVER TOXIN AND THE DICK TEST

JAMA , Volume 84 (20) – May 16, 1925

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Publisher
American Medical Association
Copyright
Copyright © 1925 American Medical Association. All Rights Reserved. Applicable FARS/DFARS Restrictions Apply to Government Use.
ISSN
0098-7484
eISSN
1538-3598
DOI
10.1001/jama.1925.02660460012006
Publisher site
See Article on Publisher Site

Abstract

The work of the Dicks and of Zingher on scarlet fever, reported from time to time in The Journal, is of great theoretical and practical importance. We have tried to repeat some of it. The toxicogenic properties of nine strains of hemolytic streptococcus, obtained from the throats of scarlet fever patients in the first days of illness, were examined. Seven of them were found to be producers of toxin. In preparing the toxin, we have used the broth of fermented meat with 2 per cent. Witte's peptone with an alkalinity of pH 7.8. Five per cent. citrated or defibrinated sheep's blood was added to the broth. After incubation for four days at 37 C., the toxin was filtered through filter paper, and 0.25 per cent. phenol (carbolic acid) was added to it. The strength of the first portions of our toxin was 1:250, and later 1:500. At our disposal

Journal

JAMAAmerican Medical Association

Published: May 16, 1925

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