ORIGINAL ARTICLE A Study of the Bacterial Microbiota in a Pediatric Patient With Chronic Serous Otitis Media Using 16SrRNA Gene-Based Pyrosequencing Cindy M. Liu, MD, MPH; Maura K. Cosetti, MD; Maliha Aziz, MS; Jordan L. Buchhagen, BS; Tania L. Contente-Cuomo, MS; Lance B. Price, PhD; Paul S. Keim, PhD; Anil K. Lalwani, MD Objective: To characterize bacterial microbiota in middle ear, tonsil, and adenoid specimens, respectively. Pseu- ear, adenoid, and tonsil specimens using 16SrRNA gene- domonadaceae dominated the middle ear microbiota at based pyrosequencing analysis. 82.7% relative abundance, whereas Streptococcaceae dominated the tonsil microbiota at 69.2%. Multiple bac- Design: Cross-sectional study of bacterial microbiota in teria, including Pseudomonadaceae, Streptococcaceae, Fu- middle ear, adenoid, and tonsil specimens from a pediat- sobacteriaceae, and Pasteurellaceae, dominated the ad- ric patient with chronic serous otitis media. Middle ear, enoid microbiota. Overlap between the middle ear and adenoid, and tonsil specimens from a pediatric patient were the tonsil microbiota was minimal. In contrast, the ad- collected and underwent cell lysis and DNA isolation. Py- enoid microbiota encompassed bacteria detected from rosequencing was performed on the 454 Life Sciences GS middle ear and tonsil. FLX platform (Roche Diagnostics Corp, Branford, Con- necticut). Pyrosequencing data were
JAMA Otolaryngology - Head & Neck Surgery – American Medical Association
Published: Jul 1, 2011
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