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The Otologic Microbiome

The Otologic Microbiome ORIGINAL ARTICLE A Study of the Bacterial Microbiota in a Pediatric Patient With Chronic Serous Otitis Media Using 16SrRNA Gene-Based Pyrosequencing Cindy M. Liu, MD, MPH; Maura K. Cosetti, MD; Maliha Aziz, MS; Jordan L. Buchhagen, BS; Tania L. Contente-Cuomo, MS; Lance B. Price, PhD; Paul S. Keim, PhD; Anil K. Lalwani, MD Objective: To characterize bacterial microbiota in middle ear, tonsil, and adenoid specimens, respectively. Pseu- ear, adenoid, and tonsil specimens using 16SrRNA gene- domonadaceae dominated the middle ear microbiota at based pyrosequencing analysis. 82.7% relative abundance, whereas Streptococcaceae dominated the tonsil microbiota at 69.2%. Multiple bac- Design: Cross-sectional study of bacterial microbiota in teria, including Pseudomonadaceae, Streptococcaceae, Fu- middle ear, adenoid, and tonsil specimens from a pediat- sobacteriaceae, and Pasteurellaceae, dominated the ad- ric patient with chronic serous otitis media. Middle ear, enoid microbiota. Overlap between the middle ear and adenoid, and tonsil specimens from a pediatric patient were the tonsil microbiota was minimal. In contrast, the ad- collected and underwent cell lysis and DNA isolation. Py- enoid microbiota encompassed bacteria detected from rosequencing was performed on the 454 Life Sciences GS middle ear and tonsil. FLX platform (Roche Diagnostics Corp, Branford, Con- necticut). Pyrosequencing data were http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png JAMA Otolaryngology - Head & Neck Surgery American Medical Association

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Publisher
American Medical Association
Copyright
Copyright 2011 American Medical Association. All Rights Reserved. Applicable FARS/DFARS Restrictions Apply to Government Use.
ISSN
2168-6181
eISSN
2168-619X
DOI
10.1001/archoto.2011.116
pmid
21768410
Publisher site
See Article on Publisher Site

Abstract

ORIGINAL ARTICLE A Study of the Bacterial Microbiota in a Pediatric Patient With Chronic Serous Otitis Media Using 16SrRNA Gene-Based Pyrosequencing Cindy M. Liu, MD, MPH; Maura K. Cosetti, MD; Maliha Aziz, MS; Jordan L. Buchhagen, BS; Tania L. Contente-Cuomo, MS; Lance B. Price, PhD; Paul S. Keim, PhD; Anil K. Lalwani, MD Objective: To characterize bacterial microbiota in middle ear, tonsil, and adenoid specimens, respectively. Pseu- ear, adenoid, and tonsil specimens using 16SrRNA gene- domonadaceae dominated the middle ear microbiota at based pyrosequencing analysis. 82.7% relative abundance, whereas Streptococcaceae dominated the tonsil microbiota at 69.2%. Multiple bac- Design: Cross-sectional study of bacterial microbiota in teria, including Pseudomonadaceae, Streptococcaceae, Fu- middle ear, adenoid, and tonsil specimens from a pediat- sobacteriaceae, and Pasteurellaceae, dominated the ad- ric patient with chronic serous otitis media. Middle ear, enoid microbiota. Overlap between the middle ear and adenoid, and tonsil specimens from a pediatric patient were the tonsil microbiota was minimal. In contrast, the ad- collected and underwent cell lysis and DNA isolation. Py- enoid microbiota encompassed bacteria detected from rosequencing was performed on the 454 Life Sciences GS middle ear and tonsil. FLX platform (Roche Diagnostics Corp, Branford, Con- necticut). Pyrosequencing data were

Journal

JAMA Otolaryngology - Head & Neck SurgeryAmerican Medical Association

Published: Jul 1, 2011

References

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