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The Influence of the Burn State on the Turnover of Serum Proteins in Human Subjects

The Influence of the Burn State on the Turnover of Serum Proteins in Human Subjects Abstract Despite the large amount of data accumulated by balance studies and by similar methods of studying the pattern of response to burns and other trauma, the basic physicochemical disturbances involved require considerable clarification, particularly the relationship of the catabolic and anabolic processes of protein metabolism. In 1952 Pollack and Halpern, in the manual on "Therapeutic Nutrition"17 sponsored by the National Research Council, set forth arguments against the theory of blockage of protein synthesis during the so-called "catabolic phase" following injury and infection, but stressed the fact that "the critical experiment designed to show whether or not any anabolism occurs" during the period "remains to be reported," adding that "the administration of an isotopically labeled amino acid during this phase and its subsequent incorporation into body proteins would prove that anabolism was taking place." Since that time the "critical experiment" has been performed many times, and it is hoped that References 1. More complete mathematical analysis of these 2. Armstrong, S. H., Jr.; Bronsky, D., and Hershman, J.: Persistence in the Blood of the Radioactive Label of Albumins, Gamma Globulins, and Globulins of Intermediate Mobility: III. Comparison of Die-away Plots Following Oral and Intravenous Administration of the S35 Label to the Same Subjects , J. Lab. & Clin. Med. 46:857, 1955. 3. Bartlett, P. D., and Gaebler, O. H.: Studies on the Mechanism of Nitrogen Storage: VI. Rate of Protein Synthesis and Size of the Nitrogen Pool , J. Biol. Chem. 196:1, 1952. 4. Blocker, T. G., Jr.; Levin, W. C.; Lewis, S. R., and Snyder, C. C.: Use of Radioactive Sulphur Labeled Methionine in the Study of Protein Catabolism in Burn Patients , Ann. Surg. 140:519, 1954. 5. Blocker, T. G., Jr.; Levin, W. C.; Nowinski, W. W.; Lewis, S. R., and Blocker, V.: Nutrition Studies in the Severely Burned , Ann. Surg. 141:589, 1955. 6. Chow, B. F.: Role of Dietary Proteins on Plasma Protein Regeneration , in Symposia on Nutrition: Plasma Proteins , edited by J. B. Youmans for the Robert Gould Research Foundation, Inc., Springfield, Ill., Charles C Thomas, publisher, 1950, Vol. 2. 7. Crispell, K. R.; Prason, W., and Hollifield, G.: Study of the Rate of Protein Synthesis Before and During the Administration of L-Triiodothyronine to Patients with Myxedema and Healthy Volunteers Using N-15 Glycine , J. Clin. Invest. 35:164, 1956. 8. Gitlin, D., and Janeway, C. A.: Dynamic Equilibrium Between Circulating and Extravascular Plasma Proteins , Science 118:301, 1953. 9. Green, H., and Anker, H. S.: Kinetics of Amino Acid Incorporation into Serum Proteins , J. Gen. Physiol. 38:283, 1955. 10. Hubay, C. A.; Krieger, H.; Holden, W. D., and Hancock, T.: Effect of Protein Depletion on Rate of Disappearance of Radioactive Protein from the Blood Stream , Proc. Soc. Exper. Biol. & Med. 78:402, 1951. 11. Lee, N. D., and Williams, R. H.: Role of the Pituitary-Adrenal System in Cystine-S35 Incorporation into Protein , Endocrinology 51:451, 1952. 12. Levin, W. C.; Blocker, T. G., Jr.; Perry, J., and Lewis, S. R.: Rate of Uptake of S35 Labeled L-Methionine by Serum Proteins in Normal and Burned Humans , Clin. Res. Proc. 3:127, 1955. 13. Levin, W. C.; Perry, J. E., and Blocker, T. G., Jr.: Adsorption of Sulfur-35 Labeled L-Methionine by Serum Proteins in Vitro , Texas Rep. Biol. & Med. 13:372, 1956. 14. Levin, W. C.; Perry, J. E.; Blocker, T. G., Jr., and Lewis, S. R.: Influence of Severe Burns on Protein Metabolism as Measured by Uptake and Excretion of L-Methionine Labeled with Radiosulfur , J. Lab. & Clin. Med. 44:830, 1954. 15. London, I. M.: Studies on the Rates of Formation of Serum Proteins in Man , in Symposia on Nutrition: Plasma Proteins , edited by J. B. Youmans for the Robert Gould Research Foundation, Inc., Springfield, Ill., Charles C Thomas, Publisher, 1950, Vol. 2. 16. Madden, S. C.: Plasma Protein Formation in Disease States , in Symposia on Nutrition: Plasma Protein , edited by J. B. Youmans for the Robert Gould Research Foundation, Inc., Springfield, Ill., Charles C Thomas, Publisher, 1950, Vol. 2. 17. McFarlane, A. S.: Use of Isotopes in the Study of Plasma Protein Metabolism , Brit. M. Bull. 8:213, 1952. 18. Pollack, H., and Halpern, S. L.: Therapeutic Nutrition, National Academy of Sciences—National Research Council , Baltimore, Williams & Wilkins Company, 1952. 19. Sellers, E. A.; You, S. S., and You, R. W.: Influence of Adrenal Cortex and Thyroid on the Loss of Nitrogen in Urine After Experimental Burns , Endocrinology 47:148, 1950. 20. Reiner, J. M.: Study of the Metabolic Turnover Rates by Means of Isotopic Tracers: I. Fundamental Relations , Arch. Biochem. 46:53, 1953. 21. Rutman, J.; Rutman, R. J., and Tarver, H.: Studies on Protein Synthesis in Vitro: V. Effect of Diet and Fasting on the Incorporation of Methionine-S35 into Liver Protein , J. Biol. Chem. 212:95, 1955. 22. San Pietro, A., and Rittenberg, D.: Study of the Rate of Protein Synthesis in Humans: II. Measurement of the Metabolic Pool and the Rate of Protein Synthesis , J. Biol. Chem. 201:457, 1953. 23. Sterling, K.: Turnover Rate of Serum Albumin in Man as Measured by I131-Tagged Albumin , J. Clin. Invest. 30:1228, 1951. 24. Steinbock, H. L., and Tarver, H.: Plasma Protein: V. Effect of the Protein Content of the Diet on Turnover , J. Biol. Chem. 209:127, 1954. 25. Volwiler, W.; Goldsworthy, P. D.; MacMartin, M. P.; Wood, P. A.; Mackay, I. R., and Fremont-Smith, K.: Biosynthetic Determination with Radioactive Sulfur of Turn-over Rates of Various Plasma Proteins in Normal and Cirrhotic Man , J. Clin. Invest. 34:1126, 1955. 26. Wasserman, K.; Joseph, J. D., and Mayerson, H. S.: Kinetics of Vascular and Extravascular Protein Exchange in Unbled and Bled Dogs , Am. J. Physiol. 184:175, 1956. 27. Whipple, G. H.: Dynamic Equilibrium of Body Proteins , Springfield, Ill., Charles C Thomas, Publisher, 1956. 28. Williamson, M. B., and Fromm, H. J.: Utilization of Sulfur Amino Acids During Healing of Experimental Wounds , Proc. Soc. Exper. Biol. & Med. 83:329, 1953. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png A.M.A. Archives Surgery American Medical Association

The Influence of the Burn State on the Turnover of Serum Proteins in Human Subjects

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Publisher
American Medical Association
Copyright
Copyright © 1957 American Medical Association. All Rights Reserved.
ISSN
0096-6908
DOI
10.1001/archsurg.1957.01280110134018
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Abstract

Abstract Despite the large amount of data accumulated by balance studies and by similar methods of studying the pattern of response to burns and other trauma, the basic physicochemical disturbances involved require considerable clarification, particularly the relationship of the catabolic and anabolic processes of protein metabolism. In 1952 Pollack and Halpern, in the manual on "Therapeutic Nutrition"17 sponsored by the National Research Council, set forth arguments against the theory of blockage of protein synthesis during the so-called "catabolic phase" following injury and infection, but stressed the fact that "the critical experiment designed to show whether or not any anabolism occurs" during the period "remains to be reported," adding that "the administration of an isotopically labeled amino acid during this phase and its subsequent incorporation into body proteins would prove that anabolism was taking place." Since that time the "critical experiment" has been performed many times, and it is hoped that References 1. More complete mathematical analysis of these 2. Armstrong, S. H., Jr.; Bronsky, D., and Hershman, J.: Persistence in the Blood of the Radioactive Label of Albumins, Gamma Globulins, and Globulins of Intermediate Mobility: III. Comparison of Die-away Plots Following Oral and Intravenous Administration of the S35 Label to the Same Subjects , J. Lab. & Clin. Med. 46:857, 1955. 3. Bartlett, P. D., and Gaebler, O. H.: Studies on the Mechanism of Nitrogen Storage: VI. Rate of Protein Synthesis and Size of the Nitrogen Pool , J. Biol. Chem. 196:1, 1952. 4. Blocker, T. G., Jr.; Levin, W. C.; Lewis, S. R., and Snyder, C. C.: Use of Radioactive Sulphur Labeled Methionine in the Study of Protein Catabolism in Burn Patients , Ann. Surg. 140:519, 1954. 5. Blocker, T. G., Jr.; Levin, W. C.; Nowinski, W. W.; Lewis, S. R., and Blocker, V.: Nutrition Studies in the Severely Burned , Ann. Surg. 141:589, 1955. 6. Chow, B. F.: Role of Dietary Proteins on Plasma Protein Regeneration , in Symposia on Nutrition: Plasma Proteins , edited by J. B. Youmans for the Robert Gould Research Foundation, Inc., Springfield, Ill., Charles C Thomas, publisher, 1950, Vol. 2. 7. Crispell, K. R.; Prason, W., and Hollifield, G.: Study of the Rate of Protein Synthesis Before and During the Administration of L-Triiodothyronine to Patients with Myxedema and Healthy Volunteers Using N-15 Glycine , J. Clin. Invest. 35:164, 1956. 8. Gitlin, D., and Janeway, C. A.: Dynamic Equilibrium Between Circulating and Extravascular Plasma Proteins , Science 118:301, 1953. 9. Green, H., and Anker, H. S.: Kinetics of Amino Acid Incorporation into Serum Proteins , J. Gen. Physiol. 38:283, 1955. 10. Hubay, C. A.; Krieger, H.; Holden, W. D., and Hancock, T.: Effect of Protein Depletion on Rate of Disappearance of Radioactive Protein from the Blood Stream , Proc. Soc. Exper. Biol. & Med. 78:402, 1951. 11. Lee, N. D., and Williams, R. H.: Role of the Pituitary-Adrenal System in Cystine-S35 Incorporation into Protein , Endocrinology 51:451, 1952. 12. Levin, W. C.; Blocker, T. G., Jr.; Perry, J., and Lewis, S. R.: Rate of Uptake of S35 Labeled L-Methionine by Serum Proteins in Normal and Burned Humans , Clin. Res. Proc. 3:127, 1955. 13. Levin, W. C.; Perry, J. E., and Blocker, T. G., Jr.: Adsorption of Sulfur-35 Labeled L-Methionine by Serum Proteins in Vitro , Texas Rep. Biol. & Med. 13:372, 1956. 14. Levin, W. C.; Perry, J. E.; Blocker, T. G., Jr., and Lewis, S. R.: Influence of Severe Burns on Protein Metabolism as Measured by Uptake and Excretion of L-Methionine Labeled with Radiosulfur , J. Lab. & Clin. Med. 44:830, 1954. 15. London, I. M.: Studies on the Rates of Formation of Serum Proteins in Man , in Symposia on Nutrition: Plasma Proteins , edited by J. B. Youmans for the Robert Gould Research Foundation, Inc., Springfield, Ill., Charles C Thomas, Publisher, 1950, Vol. 2. 16. Madden, S. C.: Plasma Protein Formation in Disease States , in Symposia on Nutrition: Plasma Protein , edited by J. B. Youmans for the Robert Gould Research Foundation, Inc., Springfield, Ill., Charles C Thomas, Publisher, 1950, Vol. 2. 17. McFarlane, A. S.: Use of Isotopes in the Study of Plasma Protein Metabolism , Brit. M. Bull. 8:213, 1952. 18. Pollack, H., and Halpern, S. L.: Therapeutic Nutrition, National Academy of Sciences—National Research Council , Baltimore, Williams & Wilkins Company, 1952. 19. Sellers, E. A.; You, S. S., and You, R. W.: Influence of Adrenal Cortex and Thyroid on the Loss of Nitrogen in Urine After Experimental Burns , Endocrinology 47:148, 1950. 20. Reiner, J. M.: Study of the Metabolic Turnover Rates by Means of Isotopic Tracers: I. Fundamental Relations , Arch. Biochem. 46:53, 1953. 21. Rutman, J.; Rutman, R. J., and Tarver, H.: Studies on Protein Synthesis in Vitro: V. Effect of Diet and Fasting on the Incorporation of Methionine-S35 into Liver Protein , J. Biol. Chem. 212:95, 1955. 22. San Pietro, A., and Rittenberg, D.: Study of the Rate of Protein Synthesis in Humans: II. Measurement of the Metabolic Pool and the Rate of Protein Synthesis , J. Biol. Chem. 201:457, 1953. 23. Sterling, K.: Turnover Rate of Serum Albumin in Man as Measured by I131-Tagged Albumin , J. Clin. Invest. 30:1228, 1951. 24. Steinbock, H. L., and Tarver, H.: Plasma Protein: V. Effect of the Protein Content of the Diet on Turnover , J. Biol. Chem. 209:127, 1954. 25. Volwiler, W.; Goldsworthy, P. D.; MacMartin, M. P.; Wood, P. A.; Mackay, I. R., and Fremont-Smith, K.: Biosynthetic Determination with Radioactive Sulfur of Turn-over Rates of Various Plasma Proteins in Normal and Cirrhotic Man , J. Clin. Invest. 34:1126, 1955. 26. Wasserman, K.; Joseph, J. D., and Mayerson, H. S.: Kinetics of Vascular and Extravascular Protein Exchange in Unbled and Bled Dogs , Am. J. Physiol. 184:175, 1956. 27. Whipple, G. H.: Dynamic Equilibrium of Body Proteins , Springfield, Ill., Charles C Thomas, Publisher, 1956. 28. Williamson, M. B., and Fromm, H. J.: Utilization of Sulfur Amino Acids During Healing of Experimental Wounds , Proc. Soc. Exper. Biol. & Med. 83:329, 1953.

Journal

A.M.A. Archives SurgeryAmerican Medical Association

Published: May 1, 1957

References