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The Immunochemistry of Middle Ear Effusions

The Immunochemistry of Middle Ear Effusions Abstract Middle ear secretions and biopsy specimens of middle ear mucosa from 50 patients with secretory otitis media were examined for distribution of major classes of immunoglobulins, immunologic localization of immunocompetent lymphoid cells, and presence and nature of antibody activity against mumps, measles, rubella, and poliomyelitis virus. Appreciable amounts of secretory component and γG, 7SγA, and Ils secretory γA immunoglobulin were regularly found in the ear fluids. Specific antibody activity in the middle ear fluid against the viruses listed was essentially limited to γA immunoglobulin, although such activity in the serum was predominantly associated with γG immunoglobulin. These data suggest the existence of a distinct secretory immune system in the middle ear. The implications of these observations may be applicable to the mechanism of protection or pathogenesis of microbial infections in the middle ear. References 1. Van de Calseyde P, Blatton V, Ampe W: The protein pattern of middle ear effusion behind an intact drum . Acta Otolaryngol 71:153-158, 1971.Crossref 2. Tonder O, Gunderson T: Nature of the fluid in serous otitis media . Arch Otolaryngol 93:473-478, 1971.Crossref 3. Juhn SK, Huff JS, Paparella MM: Biochemical analyses of middle ear effusions . Ann Otol Rhinol Laryngol 80:347-353, 1971. 4. Mogi G, Honjo S: Middle ear effusions: Analysis of protein components : Ann Otol Rhinol Laryngol 81:99-105, 1972. 5. Bernstein JM, et al: Secretory otitis media: A histopathologic and immunochemical report . Trans Am Acad Ophthamol Otolaryngol 76:1305-1317, 1972. 6. Kohn J: Cellulose acetate electrophoresis and immunodiffusion techniques , in Smith I (ed): Chromatographic and Electrophoretic Techniques . New York, Interscience Publishers Inc, 1968, vol 2. 7. Graber P, Burtin P: Immuno-Electrophoretic Analysis . New York, Elsevier Publishing Co, 1964. 8. Ouchterlony O: Diffusion-in-gel methods for immunological analysis II . Prog Allergy 6:1-29, 1962. 9. Schachman H: Ultracentrifugation in Biochemistry , New York, Academic Press, 1959. 10. Heremans JF: Immunoglobulin formation and function in different tissues . Curr Top Microbiol Immunol 45:131-203, 1968. 11. Hurlimann J, Zuber C: In vitro protein synthesis by human salivary glands . Immunology 14:809-817, 1968. 12. Porter RR: The hydrolysis of rabbit gamma globulin and antibodies with crystalline papain . Biochem J 73:119-126, 1959. 13. Tomasi TB Jr, Zigelbaum S: The selective occurrence of gamma IA globulins in certain body fluids . J Clin Invest 42:1552-1560, 1963.Crossref 14. Tomasi T, Bienenstock J: Secretory immunoglobulins . Adv Immunol 9:1-96, 1968. 15. Mancini G, Carbonara AO, Heremans JF: Immunochemicals quantitation of antigens by single radial immunodiffusion . Immunochemistry 2:235-239, 1965.Crossref 16. Siegel S: Non-Parametric Statistics . New York, McGraw-Hill Book Co Inc, 1956. 17. Walker HM, Lev J: Statistical Inference . New York, Henry Holt & Co, 1953. 18. Lennette EH, Schmidt NJ: Diagnostic Procedures For Viral and Rickettsial Disease . New York, American Public Health Assoc, 1964. 19. Ogra PL, et al: Immunoglobulin response in serum and secretions after immunization with live and inactivated poliovaccine and natural infection . N Engl J Med 279:893-900, 1968.Crossref 20. Ogra PL, et al: Antibody response in serum and nasopharynx after naturally acquired and vaccine-induced infection with rubella virus . N Engl J Med 285:1333-1339, 1971.Crossref 21. Brown GC, O'Leary TP: Fluorescent antibodies to influenza virus in various immunoglobulin fractions of serum after natural infection or vaccination . J Immunol 110:889-893, 1973. 22. Heremans JF: Biochemicals features and biologic significance of immunoglobulins , in Merlen E (ed): Immunoglobulins . Washington, DC, National Academy of Sciences, 1970, pp 53-73. 23. Ogra PL, Karzon DT: Poliovirus antibody response in serum and nasal secretions following intranasal innoculation with inactivated poliovaccine . J Immunol 102:15-23, 1969. 24. Bernstein JM, Hayes ER: The middle ear mucosa in health and disease . Arch Otolaryngol 94:30-35, 1971.Crossref 25. Burnet MF: The Clonal Selection of Acquired Immunity . London, Cambridge University Press, 1959, pp 83-86. 26. Tomasi TB, Gray HM: Structure and function of immunoglobulin A . Prog Allergy 16:81-213, 1972. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Otolaryngology American Medical Association

The Immunochemistry of Middle Ear Effusions

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Publisher
American Medical Association
Copyright
Copyright © 1974 American Medical Association. All Rights Reserved.
ISSN
0003-9977
DOI
10.1001/archotol.1974.00780030332002
Publisher site
See Article on Publisher Site

Abstract

Abstract Middle ear secretions and biopsy specimens of middle ear mucosa from 50 patients with secretory otitis media were examined for distribution of major classes of immunoglobulins, immunologic localization of immunocompetent lymphoid cells, and presence and nature of antibody activity against mumps, measles, rubella, and poliomyelitis virus. Appreciable amounts of secretory component and γG, 7SγA, and Ils secretory γA immunoglobulin were regularly found in the ear fluids. Specific antibody activity in the middle ear fluid against the viruses listed was essentially limited to γA immunoglobulin, although such activity in the serum was predominantly associated with γG immunoglobulin. These data suggest the existence of a distinct secretory immune system in the middle ear. The implications of these observations may be applicable to the mechanism of protection or pathogenesis of microbial infections in the middle ear. References 1. Van de Calseyde P, Blatton V, Ampe W: The protein pattern of middle ear effusion behind an intact drum . Acta Otolaryngol 71:153-158, 1971.Crossref 2. Tonder O, Gunderson T: Nature of the fluid in serous otitis media . Arch Otolaryngol 93:473-478, 1971.Crossref 3. Juhn SK, Huff JS, Paparella MM: Biochemical analyses of middle ear effusions . Ann Otol Rhinol Laryngol 80:347-353, 1971. 4. Mogi G, Honjo S: Middle ear effusions: Analysis of protein components : Ann Otol Rhinol Laryngol 81:99-105, 1972. 5. Bernstein JM, et al: Secretory otitis media: A histopathologic and immunochemical report . Trans Am Acad Ophthamol Otolaryngol 76:1305-1317, 1972. 6. Kohn J: Cellulose acetate electrophoresis and immunodiffusion techniques , in Smith I (ed): Chromatographic and Electrophoretic Techniques . New York, Interscience Publishers Inc, 1968, vol 2. 7. Graber P, Burtin P: Immuno-Electrophoretic Analysis . New York, Elsevier Publishing Co, 1964. 8. Ouchterlony O: Diffusion-in-gel methods for immunological analysis II . Prog Allergy 6:1-29, 1962. 9. Schachman H: Ultracentrifugation in Biochemistry , New York, Academic Press, 1959. 10. Heremans JF: Immunoglobulin formation and function in different tissues . Curr Top Microbiol Immunol 45:131-203, 1968. 11. Hurlimann J, Zuber C: In vitro protein synthesis by human salivary glands . Immunology 14:809-817, 1968. 12. Porter RR: The hydrolysis of rabbit gamma globulin and antibodies with crystalline papain . Biochem J 73:119-126, 1959. 13. Tomasi TB Jr, Zigelbaum S: The selective occurrence of gamma IA globulins in certain body fluids . J Clin Invest 42:1552-1560, 1963.Crossref 14. Tomasi T, Bienenstock J: Secretory immunoglobulins . Adv Immunol 9:1-96, 1968. 15. Mancini G, Carbonara AO, Heremans JF: Immunochemicals quantitation of antigens by single radial immunodiffusion . Immunochemistry 2:235-239, 1965.Crossref 16. Siegel S: Non-Parametric Statistics . New York, McGraw-Hill Book Co Inc, 1956. 17. Walker HM, Lev J: Statistical Inference . New York, Henry Holt & Co, 1953. 18. Lennette EH, Schmidt NJ: Diagnostic Procedures For Viral and Rickettsial Disease . New York, American Public Health Assoc, 1964. 19. Ogra PL, et al: Immunoglobulin response in serum and secretions after immunization with live and inactivated poliovaccine and natural infection . N Engl J Med 279:893-900, 1968.Crossref 20. Ogra PL, et al: Antibody response in serum and nasopharynx after naturally acquired and vaccine-induced infection with rubella virus . N Engl J Med 285:1333-1339, 1971.Crossref 21. Brown GC, O'Leary TP: Fluorescent antibodies to influenza virus in various immunoglobulin fractions of serum after natural infection or vaccination . J Immunol 110:889-893, 1973. 22. Heremans JF: Biochemicals features and biologic significance of immunoglobulins , in Merlen E (ed): Immunoglobulins . Washington, DC, National Academy of Sciences, 1970, pp 53-73. 23. Ogra PL, Karzon DT: Poliovirus antibody response in serum and nasal secretions following intranasal innoculation with inactivated poliovaccine . J Immunol 102:15-23, 1969. 24. Bernstein JM, Hayes ER: The middle ear mucosa in health and disease . Arch Otolaryngol 94:30-35, 1971.Crossref 25. Burnet MF: The Clonal Selection of Acquired Immunity . London, Cambridge University Press, 1959, pp 83-86. 26. Tomasi TB, Gray HM: Structure and function of immunoglobulin A . Prog Allergy 16:81-213, 1972.

Journal

Archives of OtolaryngologyAmerican Medical Association

Published: May 1, 1974

References

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