Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

The Human Progenitor Cell Antigen (CD34) Is Localized on Endothelial Cells, Dermal Dendritic Cells, and Perifollicular Cells in Formalin-Fixed Normal Skin, and on Proliferating Endothelial Cells and Stromal Spindle-Shaped Cells in Kaposi's Sarcoma

The Human Progenitor Cell Antigen (CD34) Is Localized on Endothelial Cells, Dermal Dendritic... Abstract The human progenitor cell antigen (CD34) is selectively expressed on hematopoietic progenitor cells in the bone marrow. In either cryostat sections of snap-frozen skin, or formalin-fixed paraffin-embedded sections of normal skin, anti-CD34 monoclonal antibody immunostained vascular endothelial cells and perivascular/interstitial dendritic cells, particularly in the reticular dermis. A distinctive population of perifollicular spindle-shaped cells in the midportion of the follicle (ie, bulge area), which is the site of the putative hair follicle stem cells, were CD34 positive, as were spindleshaped cells in and around the eccrine glands accentuating their basement membrane zone. In patch/plaque- and tumorstage acquired immunodeficiency syndrome-associated Kaposi's sarcoma lesions, CD34 expression was present on both the proliferating endothelial cells as well as the spindleshaped stromal cells. CD34 positive endothelial cells and spindle-shaped stromal cells may play important participatory and supportive functions in both normal and diseased skin. References 1. Civin CI, Strauss LC, Brovall C, Fackler MJ, Schwarty JF, Shaper JH. Antigenic analysis of hematopoiesis, III: a hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-la cells . J Immunol . 1984;133:157-165. 2. Peault BM, Thiery JP, LeDouarin NM. Surface marker for hematopoietic and endothelial cell lineages in quail that is defined by a monoclonal antibody . Proc Natl Acad Sci U S A . 1983;80:2976-2980.Crossref 3. Beschorner WE, Civin CI, Strauss LC. Localization of hematopoietic progenitor cells in tissue with the anti-My-10 monoclonal antibody . Am J Pathol . 1985;119:1-4. 4. Watt SM, Karhi K, Gatter K, et al. Distribution and epitope analysis of the cell membrane glycoprotein (HPCA-1) associated with human hematopoietic progenitor cells . Leukemia . 1987;1:417-426. 5. Nickoloff BJ, Griffiths CEM. Factor XIIIa-expressing dermal dendrocytes in AIDS-associated cutaneous Kaposi's sarcomas . Science . 1989;243:1736-1737.Crossref 6. Nickoloff BJ, Griffiths CEM. The spindle-shaped cells in cutaneous Kaposi's sarcoma: histologic simulators include factor XIIIa dermal dendrocytes . Am J Pathol . 1989;135:793-800. 7. Cerio R, Griffiths CEM, Cooper KD, Nickoloff BJ, Headington JT. Characterization of dermal dendritic cells with factor XIIIa in normal and inflamed skin . Br J Dermatol . 1989;121:421-432.Crossref 8. Mahoney SE, Duvic M, Nickoloff BJ, Lewis DE. HIV transcripts detected in HIV-associated Kaposi's sarcoma and psoriasis . J Invest Dermatol . 1990;94:552. Abstract. 9. Lipton BH, Bensch KG, Karasek MA. Reticuloendothelial properties of skin microvessel endothelial cells . J Invest Dermatol . 1990;94:550. Abstract. 10. Knowles DM, Tolidjian B, Marboe C, D'Agati V, Grimes M, Chess L. Monoclonal anti-human monocytic antibodies OKM1 and OKM5 Possess distinctive tissue distributions including differential reactivity with vascular endothelium . J Immunol . 1984;132:2170-2173. 11. Griffiths CEM, Barker JNWN, Kunkel S, Nickoloff BJ. Modulation of leukocyte adhesion molecules, a T cell chemotaxin (IL-8) and a regulatory cytokine (TNF-α) in allergic contact dermatitis (Rhus dermatitis). Br J Dermatol. In press. 12. Fivenson DP, Nickoloff BJ, Krull EA. Thy 1 + dermal dendrocytes in mycosis fungoides . Clin Res . 1990;38:835. Abstract. 13. Duijvestijn AM, Horst E, Pals ST, et al. High endothelial differentiation in human lymphoid and inflammatory tissues defined by monoclonal HECA-452 . Am J Pathol . 1988;130:147-155. 14. Hirschberg H, Moen T, Thorsby E. Antigen presenting properties of human vascular endothelial cells . J Exp Med . 1980;152: 249-253.Crossref 15. Cotsarelis G, Sun TT, Lavker RM. Label-retaining cells reside in the bulge area of pilosebaceous unit: implications for follicular stem cells, hair cycle, and skin carcinogenesis . Cell . 1990;61:1329-1337.Crossref 16. Haynes BF, Scearce RM, Lobach DF, Hensley LL. Phenotypic characterization and ontogeny of mesodermal-derived and endocrine epithelial components of the human thymic microenvironment . J Exp Med . 1984;159:1149-1168.Crossref 17. Singer KM, Scearce RM, Tuck DT, Whichard LP, Denning SM, Haynes BF. Removal of fibroblasts from human epithelial cell cultures with use of a complement fixing monoclonal antibody reactive with human fibroblasts and monocyte/macrophages . J Invest Dermatol . 1989;92:166-170.Crossref 18. Cerio R, Spaull J, Oliver GF, Wilson Jones E. A study of factor XIIIa and MAC 387 immunolabeling in normal and pathological skin . Am J Dermatopathol . 1990;12:221-233.Crossref 19. Hanson CA, Ross CW, Schnitzer B. Anti-CD34 immunoperoxidase staining in paraffin sections of acute leukemia: comparison with flow cytometric immunophenotyping. Hum Pathol. In press. 20. Schligemann RO, Rietreld FJR, Waal RMW, et al. Leukocyte antigen CD34 is expressed by a subset of cultured endothelial cells and on endothelial abluminal microprocesses in the tumor stroma . Lab Invest . 1990;62:690-696. 21. Kuo T, Uhlemann J, Reinhard EH. Cutaneous extramedullary hematopoiesis . Arch Dermatol . 1976;112:1302-1303.Crossref 22. Nickoloff BJ, Griffiths CEM. Not all spindle-shaped cells embedded in a collagenous stroma are fibroblasts: recognition of the 'collagen-associated dendrophage.' J Cutan Pathol . 1990;17:252-253.Crossref 23. Fink L, Molgaard HV, Robertson D, et al. Expression of the CD34 gene in vascular endothelial cells . Blood . 1990;75:2417-2426. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Dermatology American Medical Association

The Human Progenitor Cell Antigen (CD34) Is Localized on Endothelial Cells, Dermal Dendritic Cells, and Perifollicular Cells in Formalin-Fixed Normal Skin, and on Proliferating Endothelial Cells and Stromal Spindle-Shaped Cells in Kaposi's Sarcoma

Archives of Dermatology , Volume 127 (4) – Apr 1, 1991

Loading next page...
 
/lp/american-medical-association/the-human-progenitor-cell-antigen-cd34-is-localized-on-endothelial-7IVn5tmxlY
Publisher
American Medical Association
Copyright
Copyright © 1991 American Medical Association. All Rights Reserved.
ISSN
0003-987X
eISSN
1538-3652
DOI
10.1001/archderm.1991.04510010091009
Publisher site
See Article on Publisher Site

Abstract

Abstract The human progenitor cell antigen (CD34) is selectively expressed on hematopoietic progenitor cells in the bone marrow. In either cryostat sections of snap-frozen skin, or formalin-fixed paraffin-embedded sections of normal skin, anti-CD34 monoclonal antibody immunostained vascular endothelial cells and perivascular/interstitial dendritic cells, particularly in the reticular dermis. A distinctive population of perifollicular spindle-shaped cells in the midportion of the follicle (ie, bulge area), which is the site of the putative hair follicle stem cells, were CD34 positive, as were spindleshaped cells in and around the eccrine glands accentuating their basement membrane zone. In patch/plaque- and tumorstage acquired immunodeficiency syndrome-associated Kaposi's sarcoma lesions, CD34 expression was present on both the proliferating endothelial cells as well as the spindleshaped stromal cells. CD34 positive endothelial cells and spindle-shaped stromal cells may play important participatory and supportive functions in both normal and diseased skin. References 1. Civin CI, Strauss LC, Brovall C, Fackler MJ, Schwarty JF, Shaper JH. Antigenic analysis of hematopoiesis, III: a hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-la cells . J Immunol . 1984;133:157-165. 2. Peault BM, Thiery JP, LeDouarin NM. Surface marker for hematopoietic and endothelial cell lineages in quail that is defined by a monoclonal antibody . Proc Natl Acad Sci U S A . 1983;80:2976-2980.Crossref 3. Beschorner WE, Civin CI, Strauss LC. Localization of hematopoietic progenitor cells in tissue with the anti-My-10 monoclonal antibody . Am J Pathol . 1985;119:1-4. 4. Watt SM, Karhi K, Gatter K, et al. Distribution and epitope analysis of the cell membrane glycoprotein (HPCA-1) associated with human hematopoietic progenitor cells . Leukemia . 1987;1:417-426. 5. Nickoloff BJ, Griffiths CEM. Factor XIIIa-expressing dermal dendrocytes in AIDS-associated cutaneous Kaposi's sarcomas . Science . 1989;243:1736-1737.Crossref 6. Nickoloff BJ, Griffiths CEM. The spindle-shaped cells in cutaneous Kaposi's sarcoma: histologic simulators include factor XIIIa dermal dendrocytes . Am J Pathol . 1989;135:793-800. 7. Cerio R, Griffiths CEM, Cooper KD, Nickoloff BJ, Headington JT. Characterization of dermal dendritic cells with factor XIIIa in normal and inflamed skin . Br J Dermatol . 1989;121:421-432.Crossref 8. Mahoney SE, Duvic M, Nickoloff BJ, Lewis DE. HIV transcripts detected in HIV-associated Kaposi's sarcoma and psoriasis . J Invest Dermatol . 1990;94:552. Abstract. 9. Lipton BH, Bensch KG, Karasek MA. Reticuloendothelial properties of skin microvessel endothelial cells . J Invest Dermatol . 1990;94:550. Abstract. 10. Knowles DM, Tolidjian B, Marboe C, D'Agati V, Grimes M, Chess L. Monoclonal anti-human monocytic antibodies OKM1 and OKM5 Possess distinctive tissue distributions including differential reactivity with vascular endothelium . J Immunol . 1984;132:2170-2173. 11. Griffiths CEM, Barker JNWN, Kunkel S, Nickoloff BJ. Modulation of leukocyte adhesion molecules, a T cell chemotaxin (IL-8) and a regulatory cytokine (TNF-α) in allergic contact dermatitis (Rhus dermatitis). Br J Dermatol. In press. 12. Fivenson DP, Nickoloff BJ, Krull EA. Thy 1 + dermal dendrocytes in mycosis fungoides . Clin Res . 1990;38:835. Abstract. 13. Duijvestijn AM, Horst E, Pals ST, et al. High endothelial differentiation in human lymphoid and inflammatory tissues defined by monoclonal HECA-452 . Am J Pathol . 1988;130:147-155. 14. Hirschberg H, Moen T, Thorsby E. Antigen presenting properties of human vascular endothelial cells . J Exp Med . 1980;152: 249-253.Crossref 15. Cotsarelis G, Sun TT, Lavker RM. Label-retaining cells reside in the bulge area of pilosebaceous unit: implications for follicular stem cells, hair cycle, and skin carcinogenesis . Cell . 1990;61:1329-1337.Crossref 16. Haynes BF, Scearce RM, Lobach DF, Hensley LL. Phenotypic characterization and ontogeny of mesodermal-derived and endocrine epithelial components of the human thymic microenvironment . J Exp Med . 1984;159:1149-1168.Crossref 17. Singer KM, Scearce RM, Tuck DT, Whichard LP, Denning SM, Haynes BF. Removal of fibroblasts from human epithelial cell cultures with use of a complement fixing monoclonal antibody reactive with human fibroblasts and monocyte/macrophages . J Invest Dermatol . 1989;92:166-170.Crossref 18. Cerio R, Spaull J, Oliver GF, Wilson Jones E. A study of factor XIIIa and MAC 387 immunolabeling in normal and pathological skin . Am J Dermatopathol . 1990;12:221-233.Crossref 19. Hanson CA, Ross CW, Schnitzer B. Anti-CD34 immunoperoxidase staining in paraffin sections of acute leukemia: comparison with flow cytometric immunophenotyping. Hum Pathol. In press. 20. Schligemann RO, Rietreld FJR, Waal RMW, et al. Leukocyte antigen CD34 is expressed by a subset of cultured endothelial cells and on endothelial abluminal microprocesses in the tumor stroma . Lab Invest . 1990;62:690-696. 21. Kuo T, Uhlemann J, Reinhard EH. Cutaneous extramedullary hematopoiesis . Arch Dermatol . 1976;112:1302-1303.Crossref 22. Nickoloff BJ, Griffiths CEM. Not all spindle-shaped cells embedded in a collagenous stroma are fibroblasts: recognition of the 'collagen-associated dendrophage.' J Cutan Pathol . 1990;17:252-253.Crossref 23. Fink L, Molgaard HV, Robertson D, et al. Expression of the CD34 gene in vascular endothelial cells . Blood . 1990;75:2417-2426.

Journal

Archives of DermatologyAmerican Medical Association

Published: Apr 1, 1991

References