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The Effect of Leukocytes on Adhesion Molecules: An Explanation of Blood Transfusion Enhancement of Tumor Growth

The Effect of Leukocytes on Adhesion Molecules: An Explanation of Blood Transfusion Enhancement... Abstract Objective: To determine the mechanism of the adverse relationship between perioperative blood transfusion and lung cancer recurrence, by focusing on endothelial cell adhesion molecules (CAMs), which are thought to play a role in distant tumor cell implantation. Design and Outcome Measures: Murine endothelial cells were cocultured with allogeneic leukocytes, syngeneic leukocytes, and syngeneic lung carcinoma cells for 60 hours. The percentage of endothelial cells expressing vascular CAMs (VCAMs) and intercellular CAMs (ICAMs) was quantified during this time using indirect immunofluorescence and flow cytometry. Tumor cell adhesion to the endothelium was quantified for 6 hours using cells labeled with sulfur 35 and a scintillation counter. Setting: Laboratory. Materials: C57/BL and Balb/C mice. Results: Vascular CAM was not expressed on the endothelium, but ICAM was preferentially expressed without stimulation. Tumor-cell adhesion and endothelial ICAM expression were inversely related. After 15 hours of coculture, tumor cell adhesion was fourfold greater in the experimental group than in the control, and coincident endothelial ICAM expression was fourfold lower. Conclusion: Endothelial cell ICAM expression is negatively correlated with metastatic potential.(Arch Surg. 1996;131:438-441) References 1. Smith DM. Immunosuppressive effects of blood transfusion . Clin Lab Med . 1992;12:723-741. 2. Blumberg N, Heal JM. Effects of transfusion on immune function . Arch Pathol Lab Med . 1994;118:371-379. 3. Tartter PL. The association of perioperative blood transfusion with colorectal cancer recurrence . Ann Surg . 1992;216:633-638.Crossref 4. Busch ORC, Hop Wim CJ, Marquet RL, Jeekel J. Blood transfusions and local tumor recurrence in colorectal cancer: evidence of a noncausal relationship . Ann Surg . 1994;220:791-797.Crossref 5. Hyman NH, Foster RS Jr, Demeules JE, et al. Blood transfusions and survival after lung cancer resection . Am J Surg . 1985;149:502-507.Crossref 6. Pastorino U, Valente M, Cataldo I, et al. Perioperative blood transfusion and prognosis of resected stage la lung cancer . Eur J Cancer . 1986;22:1375-1378.Crossref 7. Keller SM, Groshen S, Martini M, Kaiser LR. Blood transfusion and lung cancer recurrence . Cancer . 1988;62:606-610.Crossref 8. Moore DWO, Piantadosi S, McKneally MF. Effect of perioperative blood transfusion on outcome in patients with surgically resected lung cancer . Ann Thorac Surg . 1989;47:346-351.Crossref 9. Little AG, Wu H-S, Ferguson MK, et al. Perioperative blood transfusion adversely affects prognosis of patients with stage I non—small-cell lung cancer . Am J Surg . 1990;160:630-633.Crossref 10. Disney APS. Complications of immunosuppression therapy in transplantation: neoplasia and infection . Med J Aust . 1992;157:262-264. 11. Filipovich AH, Spector BD, Kersey J, et al. Immunodeficiency in humans as a risk factor in the development of malignancy . Prev Med . 1980;9:252-259.Crossref 12. Parrott NR, Lennard TWJ, Proud G, et al. Blood transfusions and surgery: the effect on growth of a syngeneic sarcoma . Ann R Coll Surg Engl . 1990;72:77-81. 13. Francis DMA, Masendycz P, Clunie GJA. The timing of blood transfusion influences its effect on tumor growth . Aust N Z J Surg . 1986:56:259-260. 14. Francis DMA, Burren CP, Clunie GJA. Acceleration of B16 melanoma growth in mice after blood transfusion . Surgery . 1987;102:488-492. 15. Francis DMA, Purcell L. Enhancement of tumor growth in natural killer cell-deficient mice by allogeneic transfusion . Am J Surg . 1991;161:411-412.Crossref 16. Blajchman MA, Bardossy L, Carmen R, et al. Allogeneic blood transfusion-induced enhancement of tumor growth: two animal models showing amelioration by leukodepletion and passive transfer using spleen cells . Blood . 1993; 81:1880-1882. 17. Thiele DL, Lipsky PE. The role of cell surface recognition structures in the initiation of MHC-unrestricted 'promiscuous' killing by T cells . Immunol Today . 1989;10:375-378.Crossref 18. Halloran PF, Brosk AP, Batiuk TD, Madrenas J. The molecular immunology of acute rejection: an overview . Transpl Immunol . 1993;1:3-27.Crossref 19. Zetter BR. Adhesion molecules in tumor metastasis . Semin Cancer Biol . 1993; 4:219-299. 20. Marlin SD, Springer TA. Purified intercellular adhesion molecule-1 (ICAM-1) is a ligand for lymphocyte function associated antigen-1 (LFA-1) . Cell . 1987;51: 813-819.Crossref 21. Osborn L, Hession C, Tizard R, et al. Direct expression cloning of vascular cell adhesion molecule 1, a cytokine-induced endothelial protein that binds to lymphocytes . Cell . 1989;59:1203-1211.Crossref 22. Rice GE, Gimbrone MA, Bevilacque MP. Tumor cell endothelial interactions: increased adhesion of human melanoma cells to activated vascular endothelium . Ann Pathol . 1988;133:204-210. 23. Moroff G, Luban NL. Prevention of transfusion-associated graft-versus-host disease . Transfusion . 1992;32:102-103.Crossref 24. Lang DJ, Ebert PA, Rodgers BM, et al. Reduction of postperfusion CMV infections following the use of leukocyte depleted blood . Transfusion . 1988;17: 391-395.Crossref 25. Murthy SM, Goldschmidt RA, Rao LN, et al. The influence of surgical trauma on experimental metastasis . Cancer . 1989;64:2035-2044.Crossref 26. Takeichi M. Cadherins: a molecular family important in selective cell-cell adhesion . Science . 1991;251:1451-1455.Crossref 27. Pauli BU, Augustin-Voss HG, El-Sabban ME, et al. Organ preference of metastasis: the role of endothelial cell adhesion molecules . Cancer Metastasis Rev . 1990;9:175-189.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Surgery American Medical Association

The Effect of Leukocytes on Adhesion Molecules: An Explanation of Blood Transfusion Enhancement of Tumor Growth

Archives of Surgery , Volume 131 (4) – Apr 1, 1996

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References (34)

Publisher
American Medical Association
Copyright
Copyright © 1996 American Medical Association. All Rights Reserved.
ISSN
0004-0010
eISSN
1538-3644
DOI
10.1001/archsurg.1996.01430160096021
Publisher site
See Article on Publisher Site

Abstract

Abstract Objective: To determine the mechanism of the adverse relationship between perioperative blood transfusion and lung cancer recurrence, by focusing on endothelial cell adhesion molecules (CAMs), which are thought to play a role in distant tumor cell implantation. Design and Outcome Measures: Murine endothelial cells were cocultured with allogeneic leukocytes, syngeneic leukocytes, and syngeneic lung carcinoma cells for 60 hours. The percentage of endothelial cells expressing vascular CAMs (VCAMs) and intercellular CAMs (ICAMs) was quantified during this time using indirect immunofluorescence and flow cytometry. Tumor cell adhesion to the endothelium was quantified for 6 hours using cells labeled with sulfur 35 and a scintillation counter. Setting: Laboratory. Materials: C57/BL and Balb/C mice. Results: Vascular CAM was not expressed on the endothelium, but ICAM was preferentially expressed without stimulation. Tumor-cell adhesion and endothelial ICAM expression were inversely related. After 15 hours of coculture, tumor cell adhesion was fourfold greater in the experimental group than in the control, and coincident endothelial ICAM expression was fourfold lower. Conclusion: Endothelial cell ICAM expression is negatively correlated with metastatic potential.(Arch Surg. 1996;131:438-441) References 1. Smith DM. Immunosuppressive effects of blood transfusion . Clin Lab Med . 1992;12:723-741. 2. Blumberg N, Heal JM. Effects of transfusion on immune function . Arch Pathol Lab Med . 1994;118:371-379. 3. Tartter PL. The association of perioperative blood transfusion with colorectal cancer recurrence . Ann Surg . 1992;216:633-638.Crossref 4. Busch ORC, Hop Wim CJ, Marquet RL, Jeekel J. Blood transfusions and local tumor recurrence in colorectal cancer: evidence of a noncausal relationship . Ann Surg . 1994;220:791-797.Crossref 5. Hyman NH, Foster RS Jr, Demeules JE, et al. Blood transfusions and survival after lung cancer resection . Am J Surg . 1985;149:502-507.Crossref 6. Pastorino U, Valente M, Cataldo I, et al. Perioperative blood transfusion and prognosis of resected stage la lung cancer . Eur J Cancer . 1986;22:1375-1378.Crossref 7. Keller SM, Groshen S, Martini M, Kaiser LR. Blood transfusion and lung cancer recurrence . Cancer . 1988;62:606-610.Crossref 8. Moore DWO, Piantadosi S, McKneally MF. Effect of perioperative blood transfusion on outcome in patients with surgically resected lung cancer . Ann Thorac Surg . 1989;47:346-351.Crossref 9. Little AG, Wu H-S, Ferguson MK, et al. Perioperative blood transfusion adversely affects prognosis of patients with stage I non—small-cell lung cancer . Am J Surg . 1990;160:630-633.Crossref 10. Disney APS. Complications of immunosuppression therapy in transplantation: neoplasia and infection . Med J Aust . 1992;157:262-264. 11. Filipovich AH, Spector BD, Kersey J, et al. Immunodeficiency in humans as a risk factor in the development of malignancy . Prev Med . 1980;9:252-259.Crossref 12. Parrott NR, Lennard TWJ, Proud G, et al. Blood transfusions and surgery: the effect on growth of a syngeneic sarcoma . Ann R Coll Surg Engl . 1990;72:77-81. 13. Francis DMA, Masendycz P, Clunie GJA. The timing of blood transfusion influences its effect on tumor growth . Aust N Z J Surg . 1986:56:259-260. 14. Francis DMA, Burren CP, Clunie GJA. Acceleration of B16 melanoma growth in mice after blood transfusion . Surgery . 1987;102:488-492. 15. Francis DMA, Purcell L. Enhancement of tumor growth in natural killer cell-deficient mice by allogeneic transfusion . Am J Surg . 1991;161:411-412.Crossref 16. Blajchman MA, Bardossy L, Carmen R, et al. Allogeneic blood transfusion-induced enhancement of tumor growth: two animal models showing amelioration by leukodepletion and passive transfer using spleen cells . Blood . 1993; 81:1880-1882. 17. Thiele DL, Lipsky PE. The role of cell surface recognition structures in the initiation of MHC-unrestricted 'promiscuous' killing by T cells . Immunol Today . 1989;10:375-378.Crossref 18. Halloran PF, Brosk AP, Batiuk TD, Madrenas J. The molecular immunology of acute rejection: an overview . Transpl Immunol . 1993;1:3-27.Crossref 19. Zetter BR. Adhesion molecules in tumor metastasis . Semin Cancer Biol . 1993; 4:219-299. 20. Marlin SD, Springer TA. Purified intercellular adhesion molecule-1 (ICAM-1) is a ligand for lymphocyte function associated antigen-1 (LFA-1) . Cell . 1987;51: 813-819.Crossref 21. Osborn L, Hession C, Tizard R, et al. Direct expression cloning of vascular cell adhesion molecule 1, a cytokine-induced endothelial protein that binds to lymphocytes . Cell . 1989;59:1203-1211.Crossref 22. Rice GE, Gimbrone MA, Bevilacque MP. Tumor cell endothelial interactions: increased adhesion of human melanoma cells to activated vascular endothelium . Ann Pathol . 1988;133:204-210. 23. Moroff G, Luban NL. Prevention of transfusion-associated graft-versus-host disease . Transfusion . 1992;32:102-103.Crossref 24. Lang DJ, Ebert PA, Rodgers BM, et al. Reduction of postperfusion CMV infections following the use of leukocyte depleted blood . Transfusion . 1988;17: 391-395.Crossref 25. Murthy SM, Goldschmidt RA, Rao LN, et al. The influence of surgical trauma on experimental metastasis . Cancer . 1989;64:2035-2044.Crossref 26. Takeichi M. Cadherins: a molecular family important in selective cell-cell adhesion . Science . 1991;251:1451-1455.Crossref 27. Pauli BU, Augustin-Voss HG, El-Sabban ME, et al. Organ preference of metastasis: the role of endothelial cell adhesion molecules . Cancer Metastasis Rev . 1990;9:175-189.Crossref

Journal

Archives of SurgeryAmerican Medical Association

Published: Apr 1, 1996

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